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A new aphid genus and species (Hemiptera: Aphididae: Macrosiphini) living on ferns in Costa Rica and Mexico

Published online by Cambridge University Press:  14 June 2013

Juan M. Nieto Nafría ,
Nicolás Pérez Hidalgo ,
David Martínez-Torres  and
William Villalobos Muller
Juan M. Nieto Nafría*
Affiliation:
Departamento de Biodiversidad y Gestión Ambiental, Universidad de León, 24071 Leon, Spain
Nicolás Pérez Hidalgo
Affiliation:
Departamento de Biodiversidad y Gestión Ambiental, Universidad de León, 24071 Leon, Spain
David Martínez-Torres
Affiliation:
Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València, Apdo. Correos 22085, 46071 Valencia, Spain
William Villalobos Muller
Affiliation:
Centro de Investigación en Biología Celular y Molecular, Universidad de Costa Rica, 11501-2060, San José, Costa Rica
*
1Corresponding author (e-mail: jmnien@unileon.es). Subject editor: Patrice Bouchard
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Abstract

Aphid species colonising ferns belong to the subfamily Aphidinae (Hemiptera: Aphididae) and the majority of these to the tribe Macrosiphini. A new genus in this tribe and its type species: Gibbomyzus pteridophytorumnew genus, new species, are established. Apterous and alate viviparous females are described from specimens collected on Blechnum buchtienii Rosenstock (Blechnaceae) in Costa Rica and on Pteridium aquilinum (Linnaeus) Kuhn (Dennstaedtiaceae) and an unidentified fern in Mexico. The taxonomic validity of the two new taxa is discussed based on morphological and molecular data. Morphologically, the new genus is compared with genera with swollen siphunculi recorded in the New World, and also with genera living on ferns anywhere in the world. The identification key by Blackman and Eastop to aphids living on ferns is modified. Molecular analyses were carried out on the sequences of a fragment of the mitochondrial gene encoding for cytochrome c oxidase subunit 1 and of a fragment of the nuclear gene encoding elongation factor-1 alpha. In both analyses, G. pteridophytorumnewspecies sequences showed considerable divergence (∼4% or more) from those of 23 other species from diverse genera of Macrosiphini, supporting the conclusions of the morphological study and justifying the establishment of the new genus.

Résumé

Les espèces de pucerons qui colonisent des fougères appartiennent à la sous-famille Aphidinae (Hemiptera: Aphididae) et la majorité d'entre elles à la tribu Macrosiphini. Un nouveau genre de cette tribu et son espèce typique sont établis : Gibbomyzus pteridophytorum, nouveau genre, nouvelle espèce, avec la description des femelles vivipares aptères et ailées collectées sur Blechnum buchtienii Rosenstock (Blechnaceae) au Costa Rica, et sur Pteridium aquilinum (Linnaeus) Kuhn (Dennstaedtiaceae) et une fougère non identifiée au Mexique. La validité taxonomique des deux nouveaux taxons est discutée morphologiquement par la comparaison du nouveau genre avec des genres cités en Amérique qui présentent des cornicules gonflées, et des genres qui habitent sur des fougères dans n'importe quelle partie du monde. Une modification de la clé d'identification de Blackman et Eastop pour les pucerons qui habitent sur les fougères est présentée. Des analyses moléculaires ont été réalisées sur les séquences d'un fragment du gène mitochondrial qui code pour la sous-unité 1 du cytochrome oxydase et d'un fragment du gène nucléaire qui code pour le facteur d’élongation 1 alpha. Dans les deux analyses les séquences de G. pteridophytorumnouvelle espèce ont une divergence considérable (4% ou plus) avec celles de 23 espèces de plusieurs genres de Macrosiphini, en appuyant les conclusions de l’étude morphologique, et en justifiant qu'un nouveau genre soit établi.

Type
Systematics & Morphology
Information
The Canadian Entomologist , Volume 145 , Issue 5 , October 2013 , pp. 509 - 520
Copyright
Copyright © Entomological Society of Canada 2013 

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References

Blackman, R.L. 2010. Aphids – Aphidinae (Macrosiphini). In Handbooks for identification of British insects. Volume 2, part 7. Edited by M. Wilson. Royal Entomological Society by the Field Studies Council, London, United Kingdom. Pp. 1–413.Google Scholar
Blackman, R.L.Eastop, V.F. 2006. Aphids on the world's herbaceous plants and shrubs. Volume 1, host lists and keys; Volume 2, the aphids. J. Wiley and Sons, Chichester, United Kingdom.Google Scholar
Bonfield, J.K., Smith, K.F., Staden, R. 1995. A new DNA sequence assembly program. Nucleic Acids Research, 23: 4992–4999.CrossRefGoogle ScholarPubMed
Chakrabarti, S.Banerjee, C. 1989. Utamphorophora hymalayensis, a new aphid (Homoptera: Aphididae) infesting ferns in Western Himalaya. Proceedings of the Zoological Society, 42: 39–42.Google Scholar
Cook, E.F. 1984. Glabromyzus and Utamphorophora (Homoptera: Aphididae) species of North America. Annals of the Entomological Society of America, 77: 705–711.CrossRefGoogle Scholar
Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294–299.Google ScholarPubMed
Foottit, R.G., Halbert, S.E., Miller, G.L., Maw, E., Russell, L.M. 2006. Adventive aphids (Hemiptera: Aphididae) of America North of Mexico. Proceedings of the Entomological Society of Washington, 108: 583–610.Google Scholar
Foottit, R.G.Maw, H.E.L., von Dohlen, C.D., Hebert, P.D.N. 2008. Species identification of aphids (Insecta: Hemiptera: Aphididae) through DNA barcodes. Molecular Ecology Resources, 8: 1189–1201.CrossRefGoogle ScholarPubMed
Foottit, R.G.Richards, W.R. 1993. The genera of the aphids of Canada; Homoptera: Aphidoidea and Phylloxeroidea. In The insects and Arachnids of Canada, part 22. Agriculture Canada, Ottawa, Canada. Pp. 1–766.Google Scholar
Ghosh, A.K. 1974. Fern infesting aphids (Insecta: Homoptera) in India. Indian Journal of Horticulture, 31: 104–109.Google Scholar
Ghosh, M.R., Basu, R.C., Raychaudhuri, D.N. 1977. Studies on the aphids (Homoptera: Aphididae) from Eastern India, XXXV: three new genera and four new species from Northeast India. Oriental Insects, 11: 576–586.CrossRefGoogle Scholar
Heie, O.E. 1992. The Aphidoidea (Hemiptera) of Fennoscandia and Denmark. IV. Family Aphididae: part 1 of the tribe Macrosiphini of subfamily Aphidinae. Fauna Entomologica Scandinavica, 25: 1–190.Google Scholar
Heie, O.E. 1994. The Aphidoidea (Hemiptera) of Fennoscandia ans Denmark. V. Family Aphididae: part 2 of the tribe Macrosiphini. Fauna Entomologica Scandinavica, 28: 1–241.Google Scholar
Heie, O.E. 1995. The Aphidoidea (Hemiptera) of Fennoscandia ans Denmark. VI. Family Aphididae: part 3 of the tribe Macrosiphini of subfamily Aphidinae, and family Lachnidae. Fauna Entomologica Scandinavica, 31: 1–217.Google Scholar
Hille Ris Lambers, D. 1969. Two new aphids from Switzerland (Aphididae, Homoptera). Mitteilungen der Schweizerischen Entomologischen Gesellschaft/Bulletin de la Société Entomologique Suisse, 42: 294–304.Google Scholar
International Commission on Zoological Nomenclature 1999. International Code of Zoological Nomenclature. 4th edition. – Spanish version: Código Internacional de Nomenclatura Zoológica, cuarta edición; 2000. Consejo Superior de Investigaciones Científicas and Sociedad de amigos del Museo Nacional de Ciencias Naturales, Madrid, Spain.Google Scholar
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., et al. 2007. Clustal W and Clustal X version 2.0. Bioinformatics, 23: 2947–2948.CrossRefGoogle ScholarPubMed
Lee, S.H., Holman, J., Havelka, J. 2002. Illustrated catalogue of Aphididae in the Korean peninsula. Part I, subfamily Aphidinae (Hemiptera: Sternorrhyncha). Insects of Korea, series 9. Korea Research Institute of Bioscience and Biotechnology and Center for Insect Systematics Royal Entomological Society by the Field Studies Council, Seoul, Korea.Google Scholar
Miyazaki, M. 1968. A revision of the fern aphids of Japan with descriptions of three new species (Homoptera: Aphididae). Insecta Matsumurana (N.S.), 31: 13–24.Google Scholar
Miyazaki, M. 1971. A revision of the tribe Macrosiphini of Japan (Homoptera: Aphididae, Aphidinae). Insecta Matsumurana (N.S.), 34: 1–247.Google Scholar
Moran, N.A., Kaplan, M.E., Gelsey, M.J., Murphy, T.G., Scholes, E.A. 1999. Phylogenetics and evolution of the aphid genus Uroleucon based on mitochondrial and nuclear DNA sequences. Systematic Entomology, 24: 85–93.CrossRefGoogle Scholar
Moritsu, M. 1983. Nihon genshoku aburamushi zukan/Aphids of Japan in colors. Zenkoku Nôson Kyôiku Kyôkai, Tokyo, Japan.Google Scholar
Nieto Nafría, J.M.Favret, C. 2011. Registers of family-group and genus-group taxa of Aphidoidea/Registros de los taxones del nivel familia y del nivel género de Aphidoidea (Hemiptera Sternorrhyncha). Universidad de León, León, Spain.Google Scholar
Nieto Nafría, J.M.Mier Durante, M.P. 1988. Hemiptera Aphididae I. In Fauna Ibérica, volumen 11. Edited by M. A. Ramos et al. Museo Nacional de Ciencias Naturales, Madrid, Spain. Pp. 1–424.Google Scholar
Ratnasingham, S.Hebert, P.D.N. 2007. BOLD: the barcode of life data system. Molecular Ecology Notes, 7: 355–364.CrossRefGoogle ScholarPubMed
Remaudière, G. 1983. Pucerons nouveaux et peu connus du Mexique, 5e note: un Utamphorophora nouveau (Hom. Aphididae). Annals de la Societé Entomologique de France, 19: 227–233.CrossRefGoogle Scholar
Remaudière, G.Muñoz Viveros, A.L. 1992. Révision du genre Carolinaia et description de nouveaux taxa (Homoptera, Aphididae). Insecta Mundi, 6: 43–58.Google Scholar
Remaudière, G.Remaudière, M. 1997. Catalogue des Aphididae du monde/Catalogue of World's Aphididae. Homoptera Aphidoidea. INRA Editions, Paris, France.Google Scholar
Robinson, A.G. 1966. Review of the fern aphids in North America with descriptions of a new genus and a new species. The Canadian Entomologist, 98: 1252–1259.CrossRefGoogle Scholar
Schneider, H., Smith, A.R., Pryer, K.M. 2009. Is morphology really at odds with molecules in estimating fern phylogeny? Systematic Botany, 34: 455–475.CrossRefGoogle Scholar
Simon, S., Schierwater, B., Hadrys, H. 2010. On the value of elongation factor-1alpha for reconstructing pterygote insect phylogeny. Molecular Phylogenetics and Evolution, 54: 651–656.CrossRefGoogle ScholarPubMed
Sorin, M.Arakawa, A. 2005. A list of Aphididae of Niigata Prefecture, Japan (3). Transactions of the Essa Entomological Society Niigata, 92: 1–21.Google Scholar
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28: 2731–2739.CrossRefGoogle ScholarPubMed
Truett, A.A., Walker, J.A., Warman, M.L., Truett, G.E., Heeger, P., Mynatt, R.L. 2000. Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT). BioTechniques, 29: 52–53.CrossRefGoogle ScholarPubMed
Wilson, J.J. 2010. Assessing the value of DNA barcodes and other priority gene regions for molecular phylogenetics of Lepidoptera. PLoS One, 5: e10525.CrossRefGoogle ScholarPubMed
Wilson, J.J., Rougerie, R., Shonfeld, J., Janzen, D., Hallwachs, W., Kitching, I., et al. 2011. When species matches are unavailable are DNA barcodes correctly assigned to higher taxa? An assessment using sphingid moths. BMC Ecology, 11: 18.CrossRefGoogle ScholarPubMed