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PRECIPITIN TEST FOR EXAMINING PREDATOR–PREY INTERACTIONS IN SOYBEAN FIELDS1

Published online by Cambridge University Press:  31 May 2012

Wayne A. Gardner ,
Merle Shepard  and
Raymond Noblet
Wayne A. Gardner
Affiliation:
Department of Entomology, Fisheries, and Wildlife, Clemson University, Clemson, South Carolina 29631
Merle Shepard
Affiliation:
Department of Entomology, Fisheries, and Wildlife, Clemson University, Clemson, South Carolina 29631
Raymond Noblet
Affiliation:
Department of Entomology, Fisheries, and Wildlife, Clemson University, Clemson, South Carolina 29631
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Abstract

Precipitin test techniques were utilized for the identification of predators on three lepidopteran pests—Anticarsia gemmatalis Hübner, Heliothis zea (Boddie), and Pseudoplusia includens (Walker). The test was sensitive enough to detect these prey in a whole-body extract of one small predator or from a midgut extract of larger predators. Some cross-reactivity among the prey antigens was found which limited somewhat the applicability of the test. However, test results from field-collected predators indicated the precipitin test is a simple and rapid technique which can be used to evaluate the number of encounters between predators and prey in soybean fields.

Résumé

Le test de la précipitine a été utilisé pour l’identification des prédateurs de 3 lépidoptères nuisibles, Anticarsia gemmatalis Hübner, Heliothis zea (Boddie), et Pseudoplusia includens (Walker). Le test s’est avéré suffisamment sensible pour détecter ces proies dans un extrait global d’un seul individu pour les petits prédateurs, et dans un extrait du mésentère pour les gros prédateurs. Un certain degré de réactivité croisée a quelque peu limité l’applicabilité du test. Cependant les résultats obtenus avec des prédateurs provenant du terrain ont montré que le test de la précipitine est une méthode simple et rapide pouvant être utilisée pour évaluer la fréquence des rencontres entre prédateurs et proies dans les champs de soya.

Type
Articles
Information
The Canadian Entomologist , Volume 113 , Issue 5 , May 1981 , pp. 365 - 369
Copyright
Copyright © Entomological Society of Canada 1981

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References

Boyer, W. B. and Dumas, W. A.. 1963. Soybean insect survey as used in Arkansas. Co-op. econ. Insect Rep. 13: 91–92.Google Scholar
Brook, M. M. and Proske, H. O.. 1946. Precipitin test for determining natural insect predators of immature mosquitoes. J. natn. Malaria Soc. 5: 45–56.Google Scholar
Dempster, J. P. 1960. A quantitative study of the predators on eggs and larvae of the broom beetle, Phytodecta olivacea Forster, using the precipitin test. J. Anim. Ecol. 29: 149–167.CrossRefGoogle Scholar
Downe, A. E. R. and West, A. S.. 1954. Progress in the use of the precipitin test in entomological studies. Can. Ent. 86: 181–184.CrossRefGoogle Scholar
Fox, C. J. S. and MacLellan, C. R.. 1956. Some Carabidae and Staphylinidae shown to feed on a wireworm, Agriotes sputator (L.), by the precipitin test. Can. Ent. 88: 228–231.CrossRefGoogle Scholar
Frank, J. H. 1967. A serological method used in the investigation of the predators of the pupal stage of the winter moth, Operophtera brumata (L.) (Hydriomenidae). Quaest. ent. 3: 95–105.Google Scholar
Hall, R. R., Downe, A. E. R., MacLellan, C. R., and West, A. S.. 1953. Evaluation of insect predatorprey relationships by precipitin test studies. Mosquito News 13: 199–204.Google Scholar
Nakamura, M. and Nakamura, K.. 1977. Population dynamics of the chestnut gall wasp, Dryocosmus kuriphilus Yasumatsu (Hymenoptera:Cynipidae). V. Estimation of the effect of predation by spiders on the mortality of imaginal wasps based on the precipitin test. Oecologia (Berl.) 27: 97–116.CrossRefGoogle Scholar
Nishijima, H. 1964. Utilization of serological method in entomology [in Japanese]. Shyokubutsu Boeki 18: 429–434.Google Scholar
Rothschild, G. H. 1966. A study of a natural population of Conomelus anceps (Germar) (Homoptera:Delperacidae) including observations on predation using the precipitin test. J. Anim. Ecol. 35: 413–434.CrossRefGoogle Scholar
Shepard, M., Carner, G. R., and Turnipseed, S. G.. 1974. A comparison of three sampling methods for arthropods in soybeans. Environ. Ent. 3: 227–232.CrossRefGoogle Scholar
West, A. S. 1950. The precipitin test as an entomological tool. Can. Ent. 82: 241–244.CrossRefGoogle Scholar