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SPECIFICITY TESTING OF THE NUCLEAR POLYHEDROSIS VIRUS OF THE GYPSY MOTH, LYMANTRIA DISPAR (L.) (LEPIDOPTERA: LYMANTRIIDAE)

Published online by Cambridge University Press:  31 May 2012

K.N. Barber ,
W.J. Kaupp  and
S.B. Holmes
K.N. Barber
Affiliation:
Environmental Research and Assessment, Forest Pest Management Institute, Forestry Canada, PO Box 490, Sault Ste. Marie, Ontario, Canada P6A 5M7
W.J. Kaupp
Affiliation:
Environmental Research and Assessment, Forest Pest Management Institute, Forestry Canada, PO Box 490, Sault Ste. Marie, Ontario, Canada P6A 5M7
S.B. Holmes
Affiliation:
Environmental Research and Assessment, Forest Pest Management Institute, Forestry Canada, PO Box 490, Sault Ste. Marie, Ontario, Canada P6A 5M7
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Abstract

An aqueous suspension of the nuclear polyhedrosis virus of Lymantria dispar (L.), LdNPV, was fed to third-instar caterpillars of L. dispar and 46 species of nontarget Lepidoptera in four successive. 24- to 48-h doses of 3 × 104 polyhedral inclusion bodies (PIBs) in 2 μL applied to small pellets of artificial diet or isolated surfaces of foliage. Adults of the fly Cyrtophleba coquilletti Aldr., and adult males of the bee Megachile rotundata (Fabr.), were assayed with a single dose of 1.2 × 105 PIBs in 2 μL of 30% sucrose solution. Only those specimens that completely consumed the dose(s) were transferred to appropriate maintenance conditions for 7–10 days whereupon they were frozen. Samples of macerates of experimental specimens were dot-blotted onto nylon membranes on which whole genomic LdNPV DNA-probing and chemiluminescence techniques were used lo show presence of LdNPV DNA. With reference to positive and negative controls, the 48 nontarget species were diagnosed as nonpermissive of LdNPV but the target species was clearly infected. This study demonstrates a high degree of host-specificity of LdNPV.

Résumé

Une suspension aqueuse du virus de la polyhédrose nucléaire de Lymantria dispar (L.), LdNPV, a été offerte en nourriture à des chenilles de troisième stade de L. dispar et à des chenilles de 46 espèces non cibles, à raison de quatre doses successives 24–48 h de 3 × 104 corpuscules à inclusions polyhédriques (PIB) dans 2 μL, appliquées à de petites boulettes d’une régime alimentaire artificiel ou à des surfaces isolées de feuillage. Des adultes de la mouche Cyrtophleba coquilletti Aldr., et des mâles adultes de l’abeille Megachile rotundata (Fabr.) ont reçu une dose unique de 1,2 × 105 PIB dans 2 μL d’une solution de sucrose 30%. Seuls les spécimens qui ont consommé des doses complètes ont été mis en conditions adéquates de survie durant 7–10 jours après lesquels ils ont été ongelés. Des échantillons de spécimens expérimentaux macérés ont été soumis à la méthode du dot blot sur des membranes de nylon et la présence d’ADN de LdNPV a été éprouvée par utilisation d’une sonde moléculaire propre à reconnaître le génome complet de LdNPV et par des techniques de chimioluminescence. En comparant les résultats à des témoins positifs et négatifs, les 48 espèces non cibles ont été reconnues insensibles au LdNPV, mais l’espèce cible était nettement infectée. Cette étude met en évidence le fort de spécificité d’hôte du virus LdNPV.

[Traduit par la rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 125 , Issue 6 , December 1993 , pp. 1055 - 1066
Copyright
Copyright © Entomological Society of Canada 1993

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