The gap junction β-2 (GJB2) gene is the main gene responsible for congenital non-syndromic hearing loss. Reference Ray, Sarkar and Sable1 GJB2 encodes for a protein called connexin-26 (Cx26). Cx26 is responsible for intercellular substance transfer and signal communication and plays a critical role in hearing acquisition and maintenance. Cx26 mutations can cause not only congenital deafness but also delayed deafness. Reference Chen, Wu and Zhang2 Posterior cranial fossa malformations can arise because of any insult to this pathway occurring from early embryogenesis to neural maturation. Reference Moosavi and Kanekar3 There are many well-described malformations affecting the cerebellum and posterior cranial fossa, either syndromic (e.g., Chiari malformation or Dandy–Walker malformation (DWM)), or non-syndromic. In the following case report, we will describe brain malformations of the posterior cranial fossa in a patient carrying two biallelic pathogenic variants in the GJB2 gene, diagnosed at 40 years of age, in obvious diagnostic delay, after performing whole exome sequencing (WES). A 40-year-old patient presented to our unit for investigations regarding deafness. She started to walk independently at 12 months of age. Apparently, no hearing impairment was suspected in her first year of life. Afterward, she developed deafness/deaf-mutism, initially attributed to a possible viral infection. She also had a right hemifacial angioma spontaneously reducing over time. She also reported a previous diagnosis of glaucoma made in her country of origin, for which unfortunately documentation is lacking. In the past several years, he has been suffering from fronto-temporal tightening headache of moderate intensity not associated with dysautonomic symptoms. Attacks occur with variable frequency and increasing intensity in correlation with menstrual period. They respond to paracetamol. Brain computed tomography (CT) and magnetic resonance imaging (MRI) were performed at the age of 38 years, which disclosed the malformations and vascular changes in the posterior cranial fossa described in Figures 1 and 2, respectively.
There was no family history of malformation, deafness, or similar genetic mutation. Considering the early onset of hearing problems, genetic testing was performed. By WES, the patient was found to be compound heterozygous for two variants in the GJB2 gene: c.139G>T, (p. Glu47*), and c.35del, (p. Gly12Valfs*2), both described in the ClinVar database as pathogenic.
In the literature, patients carrying pathogenic variants in the GJB2 gene and displaying cerebellar or posterior cranial fossa malformations have been reported with DWM. Neurological symptoms vary, Reference Todt, Mazereeuw-Hautier, Binder and Willems4 as can be outlined in Table 1, summarizing previous cases from the literature.
All cases previously described in the literature have keratitis–ichthyosis–deafness (KID) syndrome. However, our patient does not meet all the diagnostic criteria. Keratitis also does not appear to be present in our patient, although she reports a previous diagnosis of glaucoma in her home country. Brain MRI, performed aged 38 years, showed posterior cranial fossa malformation, specifically vermian hypoplasia with cerebellar dysplasia and vascular dysplasia.
At present, the reason why disease-causing variants affecting Cx26 production are associated with complex brain malformations such as DWM is also unclear. Valiente et al. Reference Valiente, Ciceri, Rico and Marín8 described how focal adhesion kinase (FAK) mediates neuronal migration by regulating the assembly of Cx26-mediated adhesions in pyramidal neurons membrane. These studies were performed on mice and indicate that FAK plays a key role in the dynamic regulation of Gap-mediated adhesions during glial-driven neuronal migration. This mechanism might underlie the disruption of neuronal migration underlying posterior cranial fossa malformations found in patients harboring pathogenic GJB2 gene variants. The association between pathogenic GJB2 gene variants and posterior cranial fossa malformation seems definitely more than coincidence. The underlying pathogenic mechanism is still unclear, and we can only speculate that there is an association with FAK function, mediating neuronal migration.
Further studies, along with neuroimaging, will be critical in the future, in order to understand how mutations in this gene can result in such a brain malformation picture.
Statement of authorship
Conceptualization: CAC and CF; clinical data collection and data curation: CAC, MN, CS, DF, SR, and CF; writing – original draft preparation: CAC; writing – review and editing: CAC, CS, and CF; neuroimaging selection and description: MN; and supervision: CF. All authors have read and agreed to the published version of the manuscript.
Competing interests
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.