Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-05T13:25:15.322Z Has data issue: false hasContentIssue false
  • English
  • Français

A Simple and Reproducible Experimental in Vivo Glioma Model

Published online by Cambridge University Press:  18 September 2015

R.N. Auer ,
R.F. Del Maestro  and
R. Anderson
R.N. Auer
Affiliation:
Departments of Pathology, (Neuropathology), Brain Research Laboratory, Clinical Neurological Sciences, and Departments of Microbiology and immunology, University of Western Ontario London, Canada
R.F. Del Maestro*
Affiliation:
Departments of Pathology, (Neuropathology), Brain Research Laboratory, Clinical Neurological Sciences, and Departments of Microbiology and immunology, University of Western Ontario London, Canada
R. Anderson
Affiliation:
Departments of Pathology, (Neuropathology), Brain Research Laboratory, Clinical Neurological Sciences, and Departments of Microbiology and immunology, University of Western Ontario London, Canada
*
Brain Research Laboratory, Dept. of Clinical Neurological Sciences, Victoria Hospital, London, Ontario N6A 4G5
Rights & Permissions [Opens in a new window]

Summary:

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A simple and reproducible injection technique has been developed for inducing an in vivo experimental astrocytoma model in rats. Newborn rats were injected with a suspension of astrocytoma C-6 cells in doses ranging from 5 to 1 X 105 cells. The optimum dose of tumor cells was found to be between 104 and 105 cells, with a consistent 100% take rate seen above 1 X 104 injected cells. The injection of less than 104 cells resulted in a decreased ability to induce tumors, and a prolonged survival. The pathology was consistent with that of a glioblastoma multiforme. The tumor showed a diffuse infiltrating border, necrosis, and pseudopalisading. Light microscopy revealed undifferentiated tumor cells while electron microscopy demonstrated rough endoplasmic reticulum, Golgi complexes, mitochondria, and the occasional cilium and centriole. The nature of the astrocytoma C-6 cell line, and the advantages, disadvantages and possible uses of the model are discussed.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 8 , Issue 4 , November 1981 , pp. 325 - 331
Copyright
Copyright © Canadian Neurological Sciences Federation 1981

References

Bailey, P. and Cushing, H. (1926). A classification of the tumors of the glioma group on a histogenetic basis with a correlated study of prognosis. Philadelphia, J.B. Lippincott Co. p. 53.Google Scholar
Barker, M., Hoshino, T., Gurcay, O., et al. (1973). Development of an animal brain tumor model and its response to therapy with 1, 3-Bis (2-chloroethyl) -1-nitrosourea. Cancer Res. 33: 976–986.Google Scholar
Benda, P., Lightbody, J., Sato, G., et. al. (1968). Differentiated rat glial cell strain in tissue culture. Science 161: 370–371.Google ScholarPubMed
Benda, P., Someda, K., Messer, J., et. al. (1971). Morphological and immunochemical studies of rat glial tumors and clonal strains progagated in culture. J. Neurosurg. 34: 310–323.CrossRefGoogle Scholar
Bissel, M.G., Rubinstein, L.J., Bignami, A., et. al. (1974). Characteristics of the rat C-6 glioma maintained in organ culture systems. Production of glial fibrillary acidic protein in the absence of fibrillogenesis. Brain Res. 82: 77–89.Google Scholar
Cicero, T.J., Cowan, W.M., Moore, B.W.et. al. (1970). The cellular localization of the two brain-specific proteins, S-100 and 14-3-2. Brain Res. 18: 25–34.CrossRefGoogle ScholarPubMed
Dorfman, A. and Ho, P.L. (1970). Synthesis of acid mucopolysaccharides by glial tumor cells in tissue culture. Proc. Natl. Acad. Sci. U.S. 66: 495–499.CrossRefGoogle ScholarPubMed
Druckrey, H., Ivankovic, S., Preussmann, R., (1965). Selective induction of malignant tumors in the brain and spinal cord of rats with MNU. Z. Krebsforsch. 66: 389–408 (Ger.).Google Scholar
Druckrey, H., Ivankovic, S., Preussmann, R., (1966). Teratogenic and carcinogenic effects in the offspring after a single injection of ethylnitrosourea to pregnant rats. Nature 210: 1378–1379.CrossRefGoogle ScholarPubMed
Eagle, H., (1955). Nutrition needs of mammalian cells in tissue culture. Science 122: 501–504.Google ScholarPubMed
Haglid, K.G., Carlsson, C.A. (1971). An immunological study of some human brain tumors concerning the brain-specific protein S-100. Neurochirurgia-(Stuttg.) 14: 24–27.Google Scholar
Hart, M.N., Petito, C.K., and Earle, K.M., (1974). Mixed Gliomas. Cancer 33: 134–140.3.0.CO;2-I>CrossRefGoogle ScholarPubMed
Ivankovic, S., Druckrey, H. (1968). Transplacental induction of malignant tumors of the nervous system. 1. ENU in BD IX rats. Z. Krebsforsch. 71: 320–360 (Ger.).Google Scholar
Jänisch, W., Schreiber, D.Experimental Tumors of the Central Nervous System. First English edition, translated from German by Bigner, D.D. and Swenberg, J.A.The Upjohn Co, Kalamazoo, Michigan, 1977.Google Scholar
Kobayashi, N., Allen, N., Clendenon, N.R., et al. (1980). An improved rat brain-tumor model. J. Neurosurg. 53: 808–815.CrossRefGoogle ScholarPubMed
Mandybur, T.I., (1981). Metastatic Brain Tumors Induced by Injection of Syngeneic Tumor Cells into Cerebral Arterial Circulation in Rats. Acta Neuropathol (Berl) 53: 57–63.CrossRefGoogle Scholar
Moore, B.W., Perez, V.J., Gehring, M., (1968). Assay and regional distribution of a soluble protein characteristic of the nervous system. J. Neurochem. 15: 265–272.CrossRefGoogle ScholarPubMed
Netsky, M.G., (1963). Experimental induction and transplantation of brain tumors in animals. Acta Neurochir. (Suppl) 10: 46–55.Google Scholar
Parker, K.K., Norenberg, M.D., Vernadakis, A., (1980). “Transdifferentiation” of C6 Glial Cells in Culture. Science 208: 179–181.CrossRefGoogle ScholarPubMed
Pellegrino, L.J., Pellegrino, A.S., Cushman, A.J., (1979). A Stereotaxic Atlas of the Rat Brain. 2nd Ed. Plenum Press, New York.Google Scholar
Peters, A., Palay, S.L., and Webster, H. deF., (1976). p. 236. The Fine Structure of the Nervous System: The Neurons and Supporting Cells. W.B. Saunders Co., Philadelphia.Google Scholar
Reich, E., (1963). Biochemistry of actinomycins. Cancer Res. 23: 1428–1441.Google ScholarPubMed
Silbert, S.W., and Goldstein, M.N., (1972). Drug-induced differentiation of a rat glioma in vitro. Cancer Res. 32: 1422–1427.Google ScholarPubMed
Valenstein, E.S., (1961). A note on anesthetizing rats and guinea pigs. J. Exp. Anal. Behav. 4:6.CrossRefGoogle ScholarPubMed
Waldbaur, H., Kuschnir, H., Engelmann, U., et. al., (1978). Experiments on the Chemotherapy of a Transplantable Glioblastoma in the Rat, Employing l-(2-Chlorethyl) -3-Cyclohexyl-l-Nitrosourea (CCNU). Acta Neurochirg. 42: 103–108.CrossRefGoogle Scholar
Zanetta, J.P., Benda, P., Gombos, G., et. al., (1972). The presence of 2’, 3’ – cyclic AMP 3’-phosphohydrolase in glial cells in tissue culture. J. Neurochem. 19: 881–883.CrossRefGoogle Scholar
Zimmermann, H.M., (1955). Nature of gliomas as revealed by animal experimentation. Amer. J. Path. 31: 1–29.Google Scholar