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Analysis of SNP markers for chicken blue-shelled gene using PCR-SSCP

Published online by Cambridge University Press:  15 June 2007

Zhao Rui ,
Liu Zhen-Zhen ,
Xu Gui-Yun  and
Yang Ning
Zhao Rui
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Liu Zhen-Zhen
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Xu Gui-Yun
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Yang Ning*
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: nyang@cau.edu.cn
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Abstract

The oocyan (O) gene determines blue shell pigmentation of domesticated chicken. The O locus is closely linked to ALEV1 on chromosome 1 with a genetic distance of 2.3 cM. Blue eggshells contain a protoporphyrin, biliverdin and zinc biliverdin chelating complex, which belongs to the porphyrin metabolic pathway. A bio-informatic approach was first used to BLAST for the genes of the enzymes in the porphyrin pathway; no gene was detected to specify the O locus. Due to a limited number of simple sequence repeat (SSR) markers around the O locus, polymerase chain reaction–single-strand conformational polymorphism (PCR-SSCP) was then employed to search for candidate single nucleotide polymorphism (SNP) markers. The sequence bearing a marker was found to consist of two transversions at Chr1:61754089T→A and Chr1:61754175A→T, respectively. Population screening showed that 81% of homozygous blue-shelled layers (OO) were classified as AA genotype at the SNP loci, while 89% of heterozygous blue-shelled layers (Oo) were AB genotype and 93% of tinted layers (oo) were BB genotype. The results indicated that there is a close association between O locus and SNP locus, suggesting that the marker locus could be used as a molecular marker in breeding for blue-shelled chickens.

Keywords

oocyanblue-shelled egg-layerALEV1PCR-SSCPSNP
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 1 , April 2007 , pp. 53 - 56
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(5): 673–676

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