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Analysis of SNP markers for chicken blue-shelled gene using PCR-SSCP

Published online by Cambridge University Press:  15 June 2007

Zhao Rui ,
Liu Zhen-Zhen ,
Xu Gui-Yun  and
Yang Ning
Zhao Rui
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Liu Zhen-Zhen
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Xu Gui-Yun
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Yang Ning*
Affiliation:
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: nyang@cau.edu.cn
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Abstract

The oocyan (O) gene determines blue shell pigmentation of domesticated chicken. The O locus is closely linked to ALEV1 on chromosome 1 with a genetic distance of 2.3 cM. Blue eggshells contain a protoporphyrin, biliverdin and zinc biliverdin chelating complex, which belongs to the porphyrin metabolic pathway. A bio-informatic approach was first used to BLAST for the genes of the enzymes in the porphyrin pathway; no gene was detected to specify the O locus. Due to a limited number of simple sequence repeat (SSR) markers around the O locus, polymerase chain reaction–single-strand conformational polymorphism (PCR-SSCP) was then employed to search for candidate single nucleotide polymorphism (SNP) markers. The sequence bearing a marker was found to consist of two transversions at Chr1:61754089T→A and Chr1:61754175A→T, respectively. Population screening showed that 81% of homozygous blue-shelled layers (OO) were classified as AA genotype at the SNP loci, while 89% of heterozygous blue-shelled layers (Oo) were AB genotype and 93% of tinted layers (oo) were BB genotype. The results indicated that there is a close association between O locus and SNP locus, suggesting that the marker locus could be used as a molecular marker in breeding for blue-shelled chickens.

Keywords

oocyanblue-shelled egg-layerALEV1PCR-SSCPSNP
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 1 , April 2007 , pp. 53 - 56
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(5): 673–676

References

Bartlett, JR, Jones, CP and Smith, EJ (1996) Linkage analysis of endogenous viral element 1, blue eggshell, and pea comb loci in chickens. Journal of Heredity 87(1): 67–70.CrossRefGoogle Scholar
Bitgood, JJ, Shoffoer, RN and Otis, JS (1980) Mapping of the genes for pea comb, blue egg, barring, silver, and blood groups A, E, H, and P in the domestic fowl. Poultry Science 59: 1686–1693.CrossRefGoogle Scholar
Bitgood, JJ, Briles, RW and Briles, WE (2000) Further tests for genetic linkage of three morphological traits, three blood groups, and break points of two chromosome translations on chromosome one in the chicken. Poultry Science 79: 293–295.CrossRefGoogle Scholar
Hagenbuch, B and Meier, PJ (2003) The super family of organic anion transporting polypeptides. Biochemica et Biophysica Acta 1609: 1–18.CrossRefGoogle Scholar
International Chicken Genome Sequencing Consortium (2004) Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution. Nature 432: 695–716.CrossRefGoogle Scholar
International Chicken Polymorphism Map Consortium (2004) A genetic variation map for chicken with 2.8 million single-nucleotide polymorphisms. Nature 432(9): 717–722.CrossRefGoogle Scholar
Kennedy, GY and Ververs, HG (1973) Eggshell pigments of the Arauano fowl. Comparative Biochemistry and Physiology 44B: 11–35.Google Scholar
Kennedy, GY and Ververs, HG (1976) A survey of avian eggshell pigments. Comparative Biochemistry and Physiology 55B: 117–123.Google Scholar
Kent, WJ (2002) BLAT-the blast-like alignment tool. Genome Research 12(4): 656–664.Google ScholarPubMed
Lang, MR and Wells, JW (1987) A review of eggshell pigmentation. World Poultry Science Journal 43: 238–245.CrossRefGoogle Scholar
Sambrook, J and Russel, DW (2001) Molecular Cloning: A Laboratory Manual, 3rd ed. New York: Cold Spring Harbor Laboratory Press.Google Scholar
Yan, HX, Xie, CG, Yang, CS, Yang, N and Li, XY (2003) The selection for Xinyang Blue-shelled chicken. In: Wu, CX (editor) The Latest Development of Poultry Study. Proceedings of the 11th National Poultry Symposium. Qingdao, China: Jilin Science and Technology House, pp. 98–100 (in Chinese).Google Scholar
Yang, N, Zhao, R, Li, XY, Xu, GY, Yang, CS and Yan, HX (2003) Study of molecular markers related to blue-shelled gene in chicken. In: Wu, CX (editor) The Latest Development of Poultry Study. Proceedings of the 11th National Poultry Symposium. Qingdao, China: Jilin Science and Technology House, pp. 37–39 (in Chinese).Google Scholar