Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-12T22:51:07.916Z Has data issue: false hasContentIssue false
  • English
  • Français

Black muntjac fibroblast cell culture and interspecies embryo reconstruction in vitro

Published online by Cambridge University Press:  02 August 2007

Fang Jun-Shun ,
Tao Yong ,
Zhang Mei-Ling ,
Liu Jian-Ming ,
Han Wei ,
Zhang Zhi-Zhong  and
Zhang Xiao-Rong
Fang Jun-Shun
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Tao Yong
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Zhang Mei-Ling
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Liu Jian-Ming
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Han Wei
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Zhang Zhi-Zhong
Affiliation:
Hefei Wild Animal Park, Hefei 230061, China
Zhang Xiao-Rong*
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
*
*Corresponding author. E-mail: zxr@ahau.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

The black muntjac (Muntiacus crinifrons) is a critically endangered mammalian species, confined to a narrow region of south-eastern China. In the present study, the homogeneous ear fibroblast cells of a black muntjac were respectively cultured in Dulbecco's modified Eagle's medium (DMEM) (low glucose), DMEM (high glucose) and RPMI-1640. The population doubling time of subcultured cells was not significantly different between the three different media, but cell growth was greater in DMEM (low glucose). The interspecies embryos were reconstructed using the fibroblasts of black muntjac as donors and enucleated goat or rabbit oocytes as recipients and their blastocyst rates were 0 and 11.5%, respectively. The results demonstrated that the two cytoplasts could support reprogramming of the ear fibroblasts of black muntjac, but the developmental potential of the reconstructed embryos was different.

Keywords

black muntjacear fibroblastin vitro cultureinterspecies somatic cell nuclear transfer
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 2 , August 2007 , pp. 157 - 162
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

These authors contributed equally to this work.

First published in Journal of Agricultural Biotechnology 2007, 15(2): 228–232

References

Chen, DY, Sun, QY, Liu, JL, et al. (1999) The giant panda (Aliuropoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg. Sciences of China (C) 42: 346–353.Google Scholar
Duan, XS, Liu, AH and Lin, SY (1981) The karyotype of Muntiacus crinifrons. Zoological Research 2: 383–385 (in Chinese with English abstract).Google Scholar
Freshney, RI (1994) Disaggregation of the tissue and primary culture. In: Freshney, RI (editor) Culture of Animal Cells: A Manual of Basic Techniques. New York: Wiley-Liss and Sons, pp. 127–147.Google Scholar
Freshney, RI (2004) Culture of Animal Cells: A Manual of Basic Techniques. Beijing: Science Press, pp. 377–383 (in Chinese).Google Scholar
Guan, WJ, Ma, YH, Ding, H, et al. (2005) The establishment of fibroblast cell line and its biological characteristic research in Small Tail Han Sheep. Acta Veterinaria et Zootechnica Sinica 36: 511–516 (in Chinese with English abstract).Google Scholar
Lanza, RP, Cibelli, JB, Diaz, F, et al. (2000) Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer. Cloning 2: 79–90.CrossRefGoogle ScholarPubMed
Loi, P, Ptak, G, Barboni, B, et al. (2001) Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. Nature Biotechnology 19: 962–964.CrossRefGoogle ScholarPubMed
Ma, YH, Zhou, XM, Guan, WJ, et al. (2005) A preliminary research on Debao Pony ear marginal tissue fibroblast cell cultured by collagenase technique. Scientia Agricultura Sinica 38: 1282–1288 (in Chinese with English abstract).Google Scholar
Sheng, HL (1987) The special local animal – Black Muntjac. Chinese Journal of Zoology 22: 45–48 (in Chinese with English abstract).Google Scholar
Wu, CX (1999) The theory and technology of animal genetic resources preservation. Journal of Yunnan University 21: 7–10 (in Chinese).Google Scholar
Wu, HL and Fang, SG (2005) Mitochondrial DNA genetic diversity of black muntjac (Muntiacus crinifrons), an endangered species endemic to China. Biochemical Genetics 43(7–8): 407–416.CrossRefGoogle ScholarPubMed
Wu, HL, Jiang, H and Wu, ZA (2003) Captive husbandry of the Chinese Black Muntjac in Hefei wild animal park. Chinese Journal of Zoology 38: 40–44 (in Chinese with English abstract).Google Scholar
Xue, QS (2001) The Principles and Technology of In Vitro Culture. Beijing: Science Press. pp. 61–63 (in Chinese).Google Scholar
Zhang, M, Hou, R, Zheng, HP, et al. (2005a) Establishment and cryopreservation of a Giant Panda skin fibroblast cell line. Chinese Journal of Zoology 40: 61–67 (in Chinese with English abstract).Google Scholar
Zhang, YH, Zhang, XR, Liu, Y, et al. (2002) Culture in vitro of fibroblast cells of Boer goat and Yellow cattle's ear skin. Journal of Anhui Agricultural University 29: 387–390 (in Chinese with English abstract).Google Scholar
Zhang, ZG, Zhang, XR, Liu, Y, et al. (2005b) Study of serial nuclear transfer on goat (Bore)–rabbit inter-species cloned embryo. Scientia Agricultura Sinica 38: 601–605 (in Chinese with English abstract).Google Scholar
Zheng, X, Bao, YX, Ge, BM, et al. (2005) The current distribution and conservation status of Black Muntjac in Gutian mountain nature reserve. Journal of Natural Resources 20: 508–513 (in Chinese).Google Scholar
Zhou, XM, Ma, YH, Guan, WJ, et al. (2004) Establishment and characteristics of a Yunnan pony ear marginal fibroblast cell line. Acta Zoologica Sinica 50: 863–868 (in Chinese with English abstract).Google Scholar
Zhou, XM, Ma, YH, Guan, WJ, et al. (2005) Establishment and characteristics of a Beijing fatty chicken embryo fibroblast cell line. Acta Veterinaria et Zootechnica Sinica 36: 209–215 (in Chinese with English abstract).Google Scholar