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Black muntjac fibroblast cell culture and interspecies embryo reconstruction in vitro

Published online by Cambridge University Press:  02 August 2007

Fang Jun-Shun ,
Tao Yong ,
Zhang Mei-Ling ,
Liu Jian-Ming ,
Han Wei ,
Zhang Zhi-Zhong  and
Zhang Xiao-Rong
Fang Jun-Shun
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Tao Yong
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Zhang Mei-Ling
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Liu Jian-Ming
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Han Wei
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
Zhang Zhi-Zhong
Affiliation:
Hefei Wild Animal Park, Hefei 230061, China
Zhang Xiao-Rong*
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
*
*Corresponding author. E-mail: zxr@ahau.edu.cn
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Abstract

The black muntjac (Muntiacus crinifrons) is a critically endangered mammalian species, confined to a narrow region of south-eastern China. In the present study, the homogeneous ear fibroblast cells of a black muntjac were respectively cultured in Dulbecco's modified Eagle's medium (DMEM) (low glucose), DMEM (high glucose) and RPMI-1640. The population doubling time of subcultured cells was not significantly different between the three different media, but cell growth was greater in DMEM (low glucose). The interspecies embryos were reconstructed using the fibroblasts of black muntjac as donors and enucleated goat or rabbit oocytes as recipients and their blastocyst rates were 0 and 11.5%, respectively. The results demonstrated that the two cytoplasts could support reprogramming of the ear fibroblasts of black muntjac, but the developmental potential of the reconstructed embryos was different.

Keywords

black muntjacear fibroblastin vitro cultureinterspecies somatic cell nuclear transfer
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 2 , August 2007 , pp. 157 - 162
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

†

These authors contributed equally to this work.

First published in Journal of Agricultural Biotechnology 2007, 15(2): 228–232

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