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Cloning and sequencing of DNA components of Banana bunchy top virus Hainan isolate

Published online by Cambridge University Press:  13 June 2008

Tian E. Zhuang Jun  and
Liu Zhi-Xin
Tian E. Zhuang Jun
Affiliation:
State Key for Tropical Crop Biotechnology, Chinese Academy of Laboratory Tropical Agricultural Science, Haikou 571101, China
Liu Zhi-Xin*
Affiliation:
State Key for Tropical Crop Biotechnology, Chinese Academy of Laboratory Tropical Agricultural Science, Haikou 571101, China
*
*Corresponding author. Email: nkbltczx@public.hk.hi.cn
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Abstract

Six DNA components of Banana bunchy top virus (BBTV) were amplified by polymerase chain reaction (PCR) using total DNA from banana tissues having the typical BBTV symptoms as template. The sequence analysis indicated that the six fragments (1104 nt, 1067 nt, 1059 nt, 1045 nt, 1014 nt and 1081 nt) were all full-length components of BBTV. The viral sequences from different countries were compared. Results revealed that the sequence of component 1 was more conservative than other components, DNA1 of the Hainan isolate shared 95.4% homology with that of the Vietnam isolate, while sequences of both components 2 and 4 showed less homology. DNA2 of the Hainan isolate only shared 83% homology with that of the Guangzhou isolate. According to the results of the DNA1 sequence comparison, the BBTV Hainan isolate should belong to the Asian sub-group.

Keywords

Banana bunchy top virusene cloningsequencing
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 2 , August 2005 , pp. 91 - 97
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

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