Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-19T13:28:51.521Z Has data issue: false hasContentIssue false
  • English
  • Français

Cryopreservation of boar semen using straws

Published online by Cambridge University Press:  03 March 2009

Gao Jun-Feng ,
Zheng Xiao-Feng ,
Ge Li-Jun ,
Lu Qing  and
Rui Rong
Gao Jun-Feng
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China
Zheng Xiao-Feng
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China
Ge Li-Jun
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China
Lu Qing
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China
Rui Rong*
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China
*
*Corresponding author. E-mail: rrui@njau.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

An optimal protocol for cryopreservation of boar semen was established. First, the boar semen was pre-diluted with ZORLESCO (ZO) solution and pre-equilibrated at room temperature for 1 h. After adding extender I, spermatozoa were equilibrated at 5°C for 1.5 h; then an equal volume of extender II was added and the spermatozoa equilibrated for 2 h. The resulting spermatozoa were loaded into 0.25 ml straws, equilibrated for 10 min at 3 cm above the surface of liquid nitrogen (LN), then promptly submerged into LN. When thawing, straws were incubated in a water bath at 37°C for 30 s. This procedure yielded the highest post-thaw motility of 0.58±0.03 and plasma integrity of 63.2±1.2%, together with a normal acrosome in 51.4±2.6% of spermatozoa. Abnormal spermatozoa after freezing represented only 14.0±3.0%.

Keywords

boarsemencryopreservation
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 3 , December 2008 , pp. 231 - 236
Copyright
Copyright © China Agricultural University 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(2): 264–269

References

Bergeron, A and Manjunath, P (2006) New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Molecular Reproduction and Development 73: 1338–1344.CrossRefGoogle ScholarPubMed
Carvajaj, G, Cuello, C, Ruiz, M, et al. (2004) Effects of centrifugation before freezing on boar sperm cryosurvival. Journal of Andrology 25: 389–396.CrossRefGoogle Scholar
Cerolini, S, Maldjian, A, Pizzi, F, et al. (2001) Changes in sperm quality and lipid composition during cryopreservation of boar semen. Reproduction 121: 395–401.CrossRefGoogle ScholarPubMed
Eddy, EM and O'Brien, DA (1994) The Physiology of Reproduction. New York: Raven Press, pp. 29–77.Google Scholar
Fraser, L and Strzezek, J (2007) Effect of different procedures of ejaculate collection, extenders and packages on DNA integrity of boar spermazotoa following freezing thawing. Animal Reproduction Science 99: 317–329.CrossRefGoogle ScholarPubMed
Gadea, J, Francisco, GV, Matas, C, et al. (2005) Cooling and freezing of boar spermatozoa: Supplementation of the freezing media with reduced glutathione preserves sperm function. Journal of Andrology 26: 396–404.CrossRefGoogle ScholarPubMed
Hernandez, M, Roca, J, Gil, MA, et al. (2007) Adjustments on the cryopreservation conditions reduce the incidence of boar ejaculates with poor sperm freezability. Theriogenology 67: 1436–1445.CrossRefGoogle ScholarPubMed
Jiang, ZL, Li, G, Li, QW, et al. (2007) Effect of low density lipoprotein on DNA integrity of freezing thawing boar sperm by neutral comet assay. Animal Reproduction Science 99: 401–407.CrossRefGoogle ScholarPubMed
Jin, Y, Chen, CX, Fang, NZ, et al. (2005) Influence of trehalose supplemented in diluent on quality parameters and reactive oxygen species levels of frozen boar spermatozoa. Chinese Journal of Animal Science 41: 6–8 (in Chinese).Google Scholar
Johnson, LA, Weitze, KF, Fiser, P, et al. (2000) Storage of boar semen. Animal Reproduction Science 62: 143–172.CrossRefGoogle ScholarPubMed
Polge, C (1956) Aritificial insemination in pigs. Veterinary 68: 62–76.Google Scholar
Pursel, VG and Johnson, LA (1975) Freezing of boar spermatozoa: Fertilizing capacity with concentrated semen and a new thawing procedure. Journal of Animal Science 40: 99–102.CrossRefGoogle Scholar
Ricker, JV, Linfor, JJ, Delfino, WJ, et al. (2006) Equine sperm membrane phase behavior: the effects of lipid-based cryoprotectants. Biology of Reproduction 74: 359–365.CrossRefGoogle ScholarPubMed
Roca, J, Rodriguez, MJ, Gil, MA, et al. (2005) Survival and fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase. Journal of Andrology 26: 15–24.CrossRefGoogle ScholarPubMed
Roca, J, Vazquez, JM, Gil, MA, et al. (2006) Challenges in pig artificial insemination. Reproduction in Domestic Animals 41(Suppl. 2): 43–53.CrossRefGoogle ScholarPubMed
Su, Y, Zhou, XM, Chen, DY, et al. (1999) The mensuration of normal acrosomes of human sperm and its significance in the clinic. Jiangsu Medical Journal 25: 103–104 (in Chinese).Google Scholar
Tang, GL, Shi, WQ, Hou, YP, et al. (2006) Cryopreservation and normal temperature preservation of porcine sperm. Journal of China Agricultural University 11(4): 33–36 (in Chinese with English abstract).Google Scholar
Thurston, LM, Siggins, K, Mileham, AJ, et al. (2002) Identification of amplified restriction fragment length polymorphism markers linked to genes controlling boar sperm viability following cryopreservation. Biology of Reproduction 66: 545–554.CrossRefGoogle ScholarPubMed
Westendorf, P, Ritcher, L and Treu, H (1975) Zur tiefgefrierung von ebersperma laborund besamngsergebnisse mit demhulsenberger paillettenverfahren. Dtsch Tieraztl Wschr 82: 261–267.Google Scholar
Zhang, SS, Li, QW, Li, G, et al. (2006) Effect of trehalose, sucrose and lactose on cryopreservation of boar sperm. Journal of Northwest Sci-Tech University and Forestry (Natural Science Edition) 34: 41–50 (in Chinese with English abstract).Google Scholar
Zhou, JB, Yue, KZ, Sun, XS, et al. (2002) Studies on boar semen freezing techniques. Chinese Journal of Veterinary Science 22: 295–298 (in Chinese with English abstract).Google Scholar
Zhou, JB, Yue, SL and Yue, KZ (2005) The effect of post capacitation in vitro on the plasma membrane integrity of boar spermatozoa. Heilongjiang Animal Science and Veterinary Medicine 11: 32–33 (in Chinese).Google Scholar