Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-27T16:12:03.839Z Has data issue: false hasContentIssue false
  • English
  • Français

Direct adventitious shoot regeneration system of Euonymus fortunei var. radicans and its genetic transformation mediated by Agrobacterium tumefaciens

Published online by Cambridge University Press:  01 October 2008

Shang Ai-Qin ,
Chen Ying ,
Zhao Liang-Jun  and
Tian Ying-Chuan
Shang Ai-Qin
Affiliation:
Department of Ornamental Horticulture and Landscape Architecture, China Agriculture University, Beijing 100094, China College of Horticulture, Hebei Agricultural University, Baoding 071001, China
Chen Ying
Affiliation:
Department of Ornamental Horticulture and Landscape Architecture, China Agriculture University, Beijing 100094, China
Zhao Liang-Jun*
Affiliation:
Department of Ornamental Horticulture and Landscape Architecture, China Agriculture University, Beijing 100094, China
Tian Ying-Chuan
Affiliation:
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
*
*Corresponding author. E-mail: Zhaolj5073@sina.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Using hypocotyls as explants, the adventitious shoots of Euonymus fortunei var. radicans were differentiated directly from basal Murashige and Skoog (MS) medium supplemented with different plant growth regulators. The highest regeneration frequency was obtained with MS medium containing 0.5 mg/l 6-benzylaminopurine (BAP) and 0.01 mg/l α-naphthalene acetic acid (NAA). A regeneration frequency of 92% and 4.2 shoots per explant were obtained after 30 days of culture. The binary vector pBCGm, containing Galanthus nivalis agglutinin (GNA) gene, was introduced into Agrobacterium tumefaciens LBA4404. Hypocotyl segments of E. fortunei var. radicans were infected through A. tumefaciens-mediated transformation. Polymerase chain reaction (PCR) and PCR–Southern blot analysis results confirmed that the GNA gene was integrated into the genome of transgenic plants. The highest transformation frequency was obtained with un-precultured explants infected for 30 min with OD600=0.6 Agrobacterium tumefaciens, and co-cultivated for 3 days.

Keywords

Euonymus fortunei var. radicansadventitious shoot regenerationgenetic transformation
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 133 - 139
Copyright
Copyright © China Agricultural University 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(1): 121–126

References

Cui, ML, Ezura, H, Nishimura, S, Kamada, H and Handa, T (2004) A rapid Agrobacterium-mediated transformation of Antirrhinum majus L. by using direct shoot regeneration from hypocotyl explants. Plant Science 166: 873–879.CrossRefGoogle Scholar
De Bondt, AK, Eggermont, P, Druart, M, et al. (1994) Agrobacterium mediated transformation of apple (Malus×domestica Borkh): an assessment of factors affecting gene transferr efficiency during early transformation steps. Plant Cell Report 13: 587–593.CrossRefGoogle Scholar
Fray, A and Earle, ED (1996) An examination of factors affecting the efficiency of Agrobacterium-mediated transformation of tomato. Plant Cell Report 16: 235–240.Google Scholar
Hilder, VA, Power, KS and Gatehouse, AMR (1995) Expression of snowdrop lection in transgenic tobacco plants results in added protection against aphids. Transgenic Research 4: 18–25.CrossRefGoogle Scholar
Lu, G, Zhang, QW and Tian, YC (2004) Studies on transgenic Medicago truncatula expressing GNA gene. Plant Protection 30(6): 23–26.Google Scholar
Luo, Q, Qu, L, Cao, YL and Xu, X (2001) Preliminary study of transgenic Chinese Wolfberry with resistance to aphid. Ninxia Journal of Agriculture and Forestry Science and Technology 1: 1–3.Google Scholar
Nagori, R and Purohit, SD (2004) In vitro plantlet regeneration in Annona squamosa through direct shoot bud differentiation on hypocotyl segments. Scientia Horticulturae 99: 89–98.Google Scholar
Plastira, VA and Perdikaris, AK (1997) Effect of genotype and explant type in regeneration frequency of tomato in vitro. Acta Horticulturae 447: 235–242.Google Scholar
Quan, RD, Shang, M, Wang, ZY and Zhang, JR (2004) Introduction of snowdrop lectin gene into maize elite inbred lines via Agrobacterium tumefaciens. Acta Botanica Boreali-Occidentalia Sinica 24(5): 761–767.Google Scholar
Tai, TH and Tanksley, SD (1990) A rapid inexpensive method for isolation of total DNA from dehydrated plant tissue. Plant Molecular Biology Reporter 8(4): 297–303.CrossRefGoogle Scholar
Wang, GL and Fang, HJ (2002) Plant Genetic Engineering, 2nd ed. Beijing: Science Press.Google Scholar
Wang, GL, Liu, YH, Guo, SH, Wang, Y, Ji, Y and Fang, HJ (2004) Study on transformation of snowdrop lectin gene to Chrysanthemum and Aphis resistance of the transgenic plants. Acta Genetica Sinica 31(12): 1434–1438.Google Scholar
Wu, CY, Ye, ZB, Li, HX and Tang, KX (2000) Genetic transformation of tomato with snowdrop actin gene (GNA). Acta Botanica Sinica 42(7): 719–723.Google Scholar
Yin, SP, Jin, WM, Lu, RQ, Pan, QH and Bai, J (2004) Shoot regeneration and Agrobacterium-mediated transformation of Euonymus fortunei Hand.– Mazz. Advances in Ornamental Horticulture of China, 185–188.Google Scholar
Yuan, ZQ, Zhao, CY, Zhou, Y and Tian, YC (2001) Aphid-resistant transgenic tobacco plants expressing modified gna gene. Acta Botanica Sinica 43(6): 592–597.Google Scholar