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Evaluation of Gamma Radiation-Induced Biochemical Changes in Skin for Dose Assesment: A Study on Small Experimental Animals

Published online by Cambridge University Press:  24 May 2018

Sandeep Kumar Soni ,
Mitra Basu ,
Priyanka Agrawal ,
Aseem Bhatnagar  and
Neelam Chhillar
Sandeep Kumar Soni
Affiliation:
Department of Neuro-Biochemistry, Institute of Human Behaviour and Allied Sciences, Dilshad Garden, India
Mitra Basu
Affiliation:
Department of Nuclear Medicine, Department of Radiation Biosciences, Division of Health, Institute of Nuclear Medicine and Allied Sciences (INMAS), Defence Research & Development Organization (DRDO), Delhi, India
Priyanka Agrawal
Affiliation:
Department of Nuclear Medicine, Department of Radiation Biosciences, Division of Health, Institute of Nuclear Medicine and Allied Sciences (INMAS), Defence Research & Development Organization (DRDO), Delhi, India
Aseem Bhatnagar
Affiliation:
Department of Nuclear Medicine, Department of Radiation Biosciences, Division of Health, Institute of Nuclear Medicine and Allied Sciences (INMAS), Defence Research & Development Organization (DRDO), Delhi, India
Neelam Chhillar*
Affiliation:
Department of Neuro-Biochemistry, Institute of Human Behaviour and Allied Sciences, Dilshad Garden, India
*
Correspondence and reprint requests to Dr Neelam Chhillar, Department of Neurochemistry, Institute of Human Behavior and Allied Sciences, Dilshad Garden, Delhi 110095, India (e-mail: chhillarnlm@gmail.com).
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Abstract

Objective

Researchers have been evaluating several approaches to assess acute radiation injury/toxicity markers owing to radiation exposure. Keeping in mind this background, we assumed that whole-body irradiation in single fraction in graded doses can affect the antioxidant profile in skin that could be used as an acute radiation injury/toxicity marker.

Methods

Sprague-Dawley rats were treated with CO-60 gamma radiation (dose: 1-5 Gy; dose rate: 0.85 Gy/minute). Skin samples were collected (before and after radiation up to 72 hours) and analyzed for glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and lipid peroxidation (LPx).

Results

Intra-group comparison showed significant differences in GSH, GPx, SOD, and CAT, and they declined in a dose-dependent manner from 1 to 5 Gy (P value<0.01, r value: 0.3-0.5). LPx value increased (P value<0.01, r value: 0.3-0.5) as the dose increased, except in 1 Gy (P value>0.05).

Conclusions

This study suggests that skin antioxidants were sensitive toward radiation even at a low radiation dose, which can be used as a predictor of radiation injury and altered in a dose-dependent manner. These biochemical parameters may have wider application in the evaluation of radiation-induced skin injury and dose assessment. (Disaster Med Public Health Preparedness. 2019;13:197–202).

Keywords

radiationantioxidantskinradiation exposure
Type
Original Research
Information
Disaster Medicine and Public Health Preparedness , Volume 13 , Issue 2 , April 2019 , pp. 197 - 202
Copyright
Copyright © Society for Disaster Medicine and Public Health, Inc. 2018 

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