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Transgenic Bt-producing Brassica napus: Plutella xylostella selection pressure and fitness of weedy relatives

Published online by Cambridge University Press:  15 January 2004

Peter Mason ,
Lorraine Braun ,
Suzanne I. Warwick ,
Bin Zhu  and
C. Neal Stewart Jr.
Peter Mason
Affiliation:
Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, K. W. Neatby Bldg., Ottawa, Ontario, K1A 0C6, Canada
Lorraine Braun
Affiliation:
Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan, S7N 0X2, Canada
Suzanne I. Warwick
Affiliation:
Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, K. W. Neatby Bldg., Ottawa, Ontario, K1A 0C6, Canada
Bin Zhu
Affiliation:
Environment Canada, National Water Research Institute, 11 Innovation Blvd., Saskatoon, Saskatchewan, S7N 3H5, Canada
C. Neal Stewart Jr.
Affiliation:
Dept. of Plant Sciences and Landscape Systems, 2431 Centre Drive, Ellington Plant Sciences, University of Tennessee, Knoxville, TN 37996-4561, USA

Abstract

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Release of transgenic insect-resistant crops creates the potential not only for the insect pest to evolve resistance but for the escape of transgenes that may confer novel or enhanced fitness-related traits through hybridization with their wild relatives. The differential response of diamondback moth (Plutella xylostella) populations in eastern and western Canada to Bt-producing (GT) Brassica napus and the potential for enhanced fitness of GT B. napus and weedy GT Brassica rapa × B. napus hybrid populations (F1, BC1, BC2) were studied. Comparative bioassays using neonates and 4th instars showed that GT B. napus and GT B. rapa × B. napus hybrids are lethal to larvae from both populations. No measurable plant fitness advantage (reproductive dry weight) was observed for GT B. napus (crop) and GT B. rapa × B. napus hybrid populations at low insect pressure (1 larva per leaf). At high insect densities (>10 larvae per leaf), vegetative plant weight was not significantly different for GT B. napusand non-GT B. napus, whereas reproductive plant weight and proportion of reproductive material were significantly higher in GT B. napus. Establishment of the Bt trait in wild B. rapa populations may also increase its competitive advantage under high insect pressure.

Keywords

transgenic cropsBrassica napusoilseed rapecanolaBrassica rapaenvironmental effectsPlutella xylostelladiamondback mothBtBacillus thuringiensis
Type
Research Article
Information
Environmental Biosafety Research , Volume 2 , Issue 4 , October 2003 , pp. 263 - 276
Copyright
© ISBR, EDP Sciences, 2003

References

Asano, S, Maruyama, T, Iwasa, T, Seki, A, Takahashi, M, Soares, GG (1993) Evaluation of biological activity of Bacillus thuringiensis test samples using a diet incorporation method with diamondback moth, Plutella xylostella (Linnaeus) (Lepidoptera: Yponomeutidae). Appl. Entomol. Zool. 28: 513–524
Bing DJ, Downey RK, Rakow GFW (1996) Hybridizations among Brassica napus, B. rapa and B. juncea and their two weedy relatives B. nigra and Sinapis arvensis under open pollination conditions in the field. Plant Breed. 115: 470–473
Butts RA (1979) Some aspects of the biology and control of Plutella xylostella (L.) (Lepidoptera: Plutellidae) in southern Ontario. M.Sc. Thesis, University of Guelph, Guelph, Ontario
Canadian Corn Pest Coalition (2001) Canadian Corn Producers Unite to Protect BT Technology. http://www.pestlaw.com/x/press/2001/CCPC-20011204A.html accessed 30 April 2003
Canadian Food Inspection Agency (2002) http://www.inspection.gc.ca/english/ppc/biotech/gen/statuse.shtml accessed 3 Dec. 2002
Cao, J, Tang, JD, Strizhov, N, Shelton, AM, Earle, ED (1999) Transgenic broccoli with high levels of Bacillus thuringiensis Cry 1C protein control diamondback moth larvae resistant to Cry1A or Cry1C. Mol. Breed. 5: 131–141 CrossRef
Cho, HS, Cao, J, Ren, JP, Earle, ED (2001) Control of Lepidopteran insect pests in transgenic Chinese cabbage (Brassica rapa ssp. pekinensis) transformed with a synthetic Bacillus thuringiensis cry1C gene. Plant Cell Rep. 20: 1–7 CrossRef
Dosdall LM, Mason PG, Olfert O, Kaminski L, Keddie BA (2003) The origins of infestations of diamondback moth, Plutella xylostella (L.), in canola in western Canada. Proceedings of the Fourth International Workshop on the Management of Diamondback Moth and Other Crucifer Pests, Melbourne, Australia. In Press
Ferré J, van Rie J (2002) Biochemistry and genetics of insect resistance to Bacillus thuringiensis. Ann. Rev. Entomol. 47: 501–533
González-Cabrera, J, Herrero, S, Ferré, J (2001) High genetic variability for resistance to Bacillus thuringiensis toxins in a single population of diamondback moth. Appl. Environ. Micro. 67: 5043–5048 CrossRef
Halfhill MD, Richards HA, Mabon SA, Stewart CN Jr. (2001) Expression of GFP and Bt transgenes in Brassica napus and hybridization and introgression with Brassica rapa. Theor. Appl. Genet. 103: 659–667
Halfhill MD, Millwood RJ, Raymer PL, Stewart CN Jr. (2002) Bt-transgenic oilseed rape hybridization with its weedy relative, Brassica rapa. Environ. Biosafety Res. 1: 19–28
Hansen, LB, Siegismund, HR, Jorgenson, RB (2001) Introgression between oilseed rape (Brassica napus L.) and its weedy relative B. rapa L. in a natural population. Genet. Res. Crop Evol. 48: 621–627 CrossRef
Harcourt, DG (1960) Biology of the diamondback moth, Plutella maculipennis (Curt.) (Lepidoptera: Plutellidae), in eastern Ontario III. Natural enemies. Can. Entomol. 92: 419–428 CrossRef
Hauser, TP, Shaw, RG, Ostergård, H (1998a) Fitness of F1 hybrids between weedy Brassica rapa and oilseed rape (B. napus). Heredity 81: 429–435 CrossRef
Hauser, TP, Jorgenson, RB, Ostergård, H (1998b) Fitness of backcross and F2 hybrids between weedy Brassica rapa and oilseed rape (B. napus). Heredity 81: 436–443 CrossRef
Howard RJ, Garland JA, Seaman WL (1994) Diseases and Pests of Vegetable Crops in Canada. Entomological Society of Canada and The Canadian Phytopathological Society, Ottawa, Ontario
James C (2001) Global Review of Commercialized Transgenic Crops: 2001. ISAAA Briefs No. 24–2001 ISAAA (International Service for the Acquisition of Agri-Biotech Applications), Ithaca, NY
Jin, RG, Liu, YB, Tabashnik, BE, Borthakur, D (2000) Development of transgenic cabbage (Brassica oleracea var. capitata) for insect resistance by Agrobacterium tumefaciens-mediated transformations. In Vitro Cell. Devel. Biol. Plant 36: 231–237 CrossRef
Jorgensen, RB, Andersen, B (1994) Spontaneous hybridization between oilseed rape (Brassica napus) and weedy B. campestris (Brassicaceae): a risk of growing genetically modified oilseed rape. Amer. J. Bot. 81: 1620–1626 CrossRef
Kareiva, P, Parker, IM, Pascual, M (1996) Can we use experiments and models in predicting the invasiveness of genetically engineered organisms? Ecology 77: 1670–1675 CrossRef
Landbo L, Andersen B, Jorgensen RB (1996) Natural hybridisation between oilseed rape and a wild relative: hybrids among seeds from weedy B. campestris. Hereditas 125: 89–91
Liu, YB, Tabashnik, BE, Johnson, MW (1995) Larval age affects resistance to Bacillus thuringiensis in diamondback moth (Lepidoptera: Plutellidae). J. Econ. Entomol. 88: 788–792 CrossRef
Liu, YB, Tabashnik, BE, Meyer, SK, Crickmore, N (2001) Cross-resistance and stability of resistance to Bacillus thuringiensis toxin Cry1C in diamondback moth. Appl. Environ. Micro. 67: 3216–3219 CrossRef
McGaughey, WH, Gould, F, Gelernter, W (1998) Bt resistance management. Nat. Biotechnol. 16: 144–146 CrossRef
Metz, TD, Roush, RT, Tang, JD, Shelton, AM, Earle, ED (1995) Transgenic broccoli expressing a Bacillus thuringiensis insecticidal crystal protein: implications for pest resistance management strategies. Mol. Breed. 1: 309–317 CrossRef
Perez, CP, Shelton, AM (1997) Resistance of Plutella xylostella to Bacillus thuringiensis Berliner in central America. J. Econ. Entomol. 90: 87–93 CrossRef
Philip H, Mengersen E (1989) Insect Pests of the Prairies. University of Alberta Press, Edmonton, Alberta
Putnam LG, Burgess L (1977) Insect Pests and Diseases of Rape and Mustard. Publication No. 48, Rapeseed Association of Canada
Ramachandran S, Buntin GD, All JN, Raymer PL, Stewart CN Jr. (1998a) Greenhouse and field evaluations of transgenic canola against diamondback moth, Plutella xylostella, and corn earworm, Helicoverpa zea. Entomol. Exper. Appl. 88: 17–24
Ramachandran S, Buntin GD, All JN, Raymer PL, Stewart CN Jr. (1998b) Movement and survival of diamondback moth (Lepidoptera: Plutellidae) larvae in mixtures of nontransgenic and transgenic canola containing a cryIA (c) gene of Bacillus thuringiensis. Environ. Entomol. 27: 649–656
Ramachandran, S, Buntin, GD, All, JN, Tabashnik, BE, Raymer, PL, Adang, MJ, Pulliam, DA, Stewart, CN Jr (1998c) Survival, development, and oviposition of resistant diamondback moth (Lepidoptera: Plutellidae) on transgenic canola producing a Bacillus thuringiensis toxin. J. Econ. Entomol. 91: 1239–1244 CrossRef
Roush, RT (1994) Managing pests and their resistance to Bacillus thuringiensis: can transgenic crops be better than sprays? Biocontrol. Sci. Technol. 4: 501–516 CrossRef
SAS Institute Inc. (1989) SAS/STAT $^{\circledR}$ users guide, version 6, 4th edition, volume 2. Cary, North Carolina: SAS Institute Inc.
Sayyed AH, Haward R, Herrero S, Ferré J, Wright DJ (2000) Genetic and biochemical approach for characteriza- tion of resistance to Bacillus thuringiensis toxin Cry1Ac in a field population of the diamondback moth, Plutella xylostella. Appl. Environ. Micro. 66: 1509–1516
Shelton, AM, Cooley, RJ, Kroening, MK, Wilsey, WT, Eigenbrode, SD (1991) Comparative analysis of two rearing procedures for diamondback moth. J. Entomol. Sci. 26: 17–26
Shelton, AM, Robertson, JL, Tang, JD, Perez, C, Eigenbrode, SD, Preisler, HK, Wilsey, WT, Cooley, RJ (1993) Resistance of diamondback moth (Lepidoptera: Plutellidae) to Bacillus thuringiensis subspecies in the field. J. Econ. Entomol. 86: 697–705 CrossRef
Snow, AA (2002) Transgenic crops – why gene flow matters. Nat. Biotechnol. 20: 542 CrossRef
Snow, AA, Pilson, D. Rieseberg, LH, Paulsen, MJ, Pleskac, N, Reagon, MR, Wolf, DE, Selbo, SM (2003) A Bt transgene reduces herbivory and enhances fecundity in wild sunflowers. Ecol. Applications 13: 279–286 CrossRef
Stewart, CN Jr., Adang, MJ, All, JN, Raymer, PL, Ramachandran, S, Parrott, WA (1996) Insect control and dosage effects in transgenic canola containing a synthetic Bacillus thuringiensis cryIAc gene. Plant Physiol. 112: 115–120 CrossRef
Stewart, CN Jr., All, JN, Raymer, PL, Ramachandran, S (1997) Increased fitness of transgenic insecticidal rapeseed under insect selection pressure. Mol. Ecol. 6: 773–779 CrossRef
Tabashnik BE (1994) Evolution of resistance to Bacillus thuringiensis. Ann. Rev. Entomol. 39: 47–79
Tabashnik, BE, Liu, YB, Finson, N, Masson, L, Heckel, DG (1997a) One gene in diamondback moth confers resistance to four Bacillus thuringiensis toxins. Proc. Nat. Acad. Sci. USA 94: 12780–12785 CrossRef
Tabashnik BE, Liu YB, Malvar T, Heckel DG, Masson L, Ballester V, Granero F, Ménsau JL, Ferré J (1997b) Global variation in the genetic and biochemical basis of diamondback moth resistance to Bacillus thuringiensis. Proc. Nat. Acad. Sci. USA 94: 12780–12785
van Raamsdonk, LWD, Schouten, HJ (1997) Gene flow and establishment of transgenes in natural plant populations. Acta Bot. Neerlandica 46: 69–84 CrossRef
Warwick, SI, Beckie, H, Small, E (1999) Transgenic crops: new weed problems for Canada. Phytoprotection 80: 71–84 CrossRef
Warwick, SI, Beckie, HJ, Thomas, G, McDonald, T (2000) The biology of Canadian weeds. 8. Sinapis arvensis L. (updated). Can. J. Plant Sci. 80: 939–961 CrossRef
Warwick, SI, Francis, A, Susko, DJ (2002) The biology of Canadian weeds. 9. Thlaspi arvense L. (updated). Can. J. Plant Sci. 82: 803–823 CrossRef
Warwick, SI, Simard, M-J, Légère, A, Beckie, HJ, Braun, L, Mason, P, Zhu, B, Séguin-Swartz, G, Stewart, CN Jr (2003) Hybridization between transgenic Brassica napus L. and its wild relatives: B. rapa L., Raphanus raphanistrum L., Sinapis arvensis L., and Erucastrum gallicum (Willd.) O. E. Schulz. Theor. Appl. Genet. 107: 528–539 CrossRef
Western Committee on Crop Pests (1995) Minutes of the 34th Annual Meeting, October 19–21, 1995. Victoria, British Columbia
Western Committee on Crop Pests (2001) Minutes of the 41st Annual Meeting, October 15–16, Banff, Alberta
Xiang, Y, Wong, WKR, Ma, MC, Wong, RSC (1999) Agrobacterium-mediated transformation of Brassica campestris ssp. parachinensis with synthetic Bacillus thuringiensis cry1Ab and cry1Ac genes. Plant Cell Rep. 19: 251–256 CrossRef