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An Improved Method for the Concentration of Antitoxic Sera

Published online by Cambridge University Press:  15 May 2009

Annie Homer
Annie Homer
Affiliation:
(From The Lister Institute, Elstree.)
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The method for the concentration of antitoxic sera suggested in this communication and now being used in this laboratory, presents a further step towards the desired goal, viz. the preparation, on a commercial scale for general therapeutic use, of antitoxic sera with a minimal amount of attendant protein.

In the concentration of sera on a large scale by the routine methods hitherto published the final products have shown a protein content of about 18 to 20 % with a potency per c.c. four to five times that of the original serum. The above described method yields, as a matter of routine, a final product with a protein content of about 17 to 19% and with a potency per c.c. as much as nine times that of the original serum. With the use of better processes the potency might be higher still.

It is worth while to concentrate by this method plasma or sera in which the unitage is so low that hitherto they would have been discarded as having too low a potency for use even after concentration.

The patient will receive a relatively higher percentage of antitoxic units per gram of protein than if the sera had been concentrated by other methods, for by this method the removal of 70% of the total proteins is ensured.

The heat denaturation of the proteins during the prolonged heating of the serum and consequent diminution of protein relatively to antitoxin may serve to minimise troubles with serum sickness.

Type
Research Article
Information
Journal of Hygiene , Volume 15 , Issue 3 , September 1916 , pp. 388 - 400
Copyright
Copyright © Cambridge University Press 1916

References

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