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The assessment and application of a bacteriocin typing scheme for Clostridium perfringens

Published online by Cambridge University Press:  19 October 2009

G. N. Watson
G. N. Watson
Affiliation:
Food Hygiene Laboratory, Central Public Health Laboratory, London NW9 5HT
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Summary

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A collection of 50 bacteriocins was assembled and used to type 802 isolates of Clostridium perfringens from food poisoning outbreaks and a variety of other sources. It was found that strains of the same serotype within an outbreak showed similar patterns of susceptibility to bacteriocins, and the use of a ‘one difference’ rule is proposed for interpretation of the typing patterns of epidemiologically related strains. Isolates of different serotype or of the same serotype isolated from different sources produced many variations in bacteriocin susceptibility patterns.

Two computer programs were developed to assist in the interpretation of bacteriocin typing patterns. Their use showed that related and unrelated strains formed different clusters and enabled a range of the 20 most discriminatory bacteriocins to be selected.

Isolates of C. perfringens from a wide range of sources were screened for their ability to produce bacteriocins. A much greater proportion of the strains from food poisoning outbreaks was bacteriocinogenic than were isolates from human and animal infections, various foods and the environment. The relevance of these findings to the occurrence of C. perfringens food poisoning is discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 94 , Issue 1 , February 1985 , pp. 69 - 79
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Abbott, J. D. & Shannon, R. (1958). A method for typing Shigella sonnet using colicin production as a marker. Journal of Clinical Pathology 11, 71–77.CrossRefGoogle Scholar
Anderhub, B., Pitt, T. L., Erdman, Y. J. & Willcox, W. R. (1977). The comparison of typing methods for Serratia marcescens. Journal of Hygiene 79, 89–102.CrossRefGoogle ScholarPubMed
Antohi, Maria (1977). Lea welchicines. In Actual Data on the Biology and Pathology of Anaerobic Bacteria, pp. 229–233. Bucharest: Medical Publishing House.Google Scholar
Bartholomew, , Barbara, A. & Stringer, M. F. (1983). Application of an ELISA technique for the detection of Clostridium perfringens enterotoxin. Journal of Applied Bacteriology 55, ix.Google Scholar
Bittner, J., Antohi, Maria, Voinescu, Viorica, Nicolescu, Mariana & Badita, Gh. (1980). Clostridium perfringens food-borne disease and bacteriological analysis for strain identification. Archives Jioumaines de Paihologie Experimental et de Microbiologie 39, 95–103.Google ScholarPubMed
Edmondson, A. S. & Cooke, E. Mary (1979). The development and assessment of a bacteriocin typing method for Klebsiella. Journal of Hygiene 82, 207–223.CrossRefGoogle ScholarPubMed
Hall, F. A. (1971). Bacteriocin typing of Klebsiella species. Journal of Clinical Pathology 24, 712–716.CrossRefGoogle Scholar
Hardy, K. G. (1982). Bacteriocins. In Experimental Microbial Ecology (ed. by Burns, R. G. and Slater, J. H.), pp. 368–378. Oxford: Blackwell.Google Scholar
Mahony, D. E. (1974). Bacteriocin susceptibility of Clostridium perfringens: a provisional typing schema. Applied Microbiology 28, 172–176.CrossRefGoogle ScholarPubMed
Mahony, D. E. & Li, A. (1978). Comparative study of ten bacteriocins of Clostridium perfringens. Antimicrobial Agents and Chemotherapy 14, 886–892.CrossRefGoogle ScholarPubMed
Mahony, D. E. & Swantee, C. A. (1978). Bacteriocin typing of Clostridium perfringens in human feces. Journal of Clinical Microbiology 7, 307–309.CrossRefGoogle ScholarPubMed
Mayr-Harting, Anna, Hedges, A. J. & Berkeley, H. C. W. (1972). Methods for studying bncteriocins. In Methods in Microbiology, vol. 7 A (ed. Norris, J. R. and Ribbons, D. W.), pp. 315–422. London: Academic Press.Google Scholar
Moors, D. C., Haldane, E. V., Martin, R. S. & Sumarah, R. (1980). Two episodes of food poisoning due to Clostridium perfringens – Nova Scotia. Canadian Diseases Weekly Report 6, 230.Google Scholar
Riley, T. V. & Mee, B. J. (1981). Simple method for detecting liacteriodes spp. bacteriocin production. Journal of Clinical Microbiology 13, 594–595.CrossRefGoogle ScholarPubMed
Satija, K. C. & Narayan, K. G. (1980 a). Active perfringocin typing of food poisoning strains of Clostridium perfringens type A – a new tool forepidemiological investigations. International Journal of Zoonoses 7, 78–84.Google Scholar
Satija, K. C. & Narayan, K. G. (1980 b). Passive bacteriocin typing of strains of Clostridium perfringens type A causing food poisoning for epidemiologic studies. Journal of Infectious Diseases 142, 899–902.CrossRefGoogle ScholarPubMed
Scott, , Heather, G. & Mahony, D. E. (1982). Further development of a bacteriocin typing system for Clostridium perfringens. Journal of Applied Bacteriology 53, 363–369.Google Scholar
Slmoons-Smit, A. M., Vkrweij-Van Vuoht, A. M. J. J., Kanis, I. Y. R. & Maclaren, D. M. (1983). Comparison of different methods for bacteriocin typing of Klebsiella strains. Journal of Hygiene 90, 461–473.CrossRefGoogle Scholar
Smith, H. W. (1959). The bacteriophages of Clostridium perfringens. Journal of General Microbiology 21, 622–630.CrossRefGoogle ScholarPubMed
Sneath, P. H. A. & Sokal, R. R. (1962). Numerical taxonomy. Nature (London) 193, 855–860.Google Scholar
Sneath, P. H. A. & Sokal, R. R. (1973). Numerical Taxonomy. The Principles and Practice of Numerical Classification. San Francisco: Freeman.Google Scholar
Stringer, M. F., Turnbull, P. C. B. & Gilbert, R. J. (1980). Application of serological typing to the investigation of outbreaks of Clostridium perfringens food poisoning, 1970–1978. Journal of Hygiene 84, 443–456.Google Scholar
Stringer, M. F., Watson, G. N. & Gilbert, R. J. (1982). Clostridium perfringens type A: serological typing and methods for the detection of enterotoxin. In Methods for the Isolation and Identification of Food Poisoning Organisms. Society for Applied Bacteriology Technical Series No. 17 (ed. Corry, Janet E. L., Roberts, Diane and Skinner, F. A.), pp. 111–135. London: Academic Press.Google Scholar
Uchiyama, K. (1966). Studies on ‘Bacteriocin-like substance’ produced by Clostridium perfringens. (1) Activity spectrum of the inhibitory substance produced by Clostridium perfringens. Medical Journal of Kagoshima University 18, 131–144.Google Scholar
Watson, G. N. (1983). The development and application of a bacteriocin typing scheme for Closlridium perfringens. M.Phil, thesis, Council for National Academic Awards.Google Scholar
Watson, G. N., Stringer, M. F., Gilbert, R. J. & Mahony, D. E. (1982). The potential of bacteriocin typing in the study of Clostridium perfringens food poisoning. Journal of Clinical Pathology 35, 1361–1365.CrossRefGoogle Scholar