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Characterization of R-plasmids coding for ampicillin resistance from Salmonella species

Published online by Cambridge University Press:  15 May 2009

A. F. Jamieson  and
M. J. Green
A. F. Jamieson
Affiliation:
Departments of Pathology and Cell Biology, University of Auckland, Auckland, New Zealand
M. J. Green
Affiliation:
National Health Institute, Wellington, New Zealand
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A sudden increase in the incidence of ampicillin resistance was observed among Salmonella species isolated within New Zealand in 1973–4. This increase was due mainly to the appearance and proliferation of Salmonella newington and Salmonella anatum serotypes resistant to ampicillin. The plasmid complements of 14 ampicillin-resistant S. newington and S. anatum isolates obtained from widely separated geographical areas within New Zealand between 1973 and 1974 were characterized by agarose gel electrophoresis. Each contained one or more plasmids ranging in molecular weight from 1·4 to 42 Mdal. Eleven isolates contained a self-transmissible plasmid of 33 Mdal which encoded resistance to ampicillin. After transfer to Escherichia coli, the 33 Mdal R-plasmids from each of these isolates were shown to be identical by restriction endonuclease analysis. The remaining three strains contained ampicillin R-plasmids having molecular weights of 35, 37·5 and 42 Mdal. These plasmids were shown by restriction endonuclease analysis to be related to the 33 Mdal R-plasmid. We conclude that the 33 Mdal plasmid and its derivatives were responsible for the increase in the incidence of ampicillin-resistant S. newington and S. anatum serotypes among the total Salmonella population.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 2 , October 1979 , pp. 371 - 376
Copyright
Copyright © Cambridge University Press 1979

References

Achtman, M. & Helmuth, R. (1975). The F factor carries an operon of more than 15 x 106 daltons coding for deoxyribonucleic acid transfer and surface exclusion. In Microbiology – 1974 (ed. Schlessinger, D.), pp. 95–103. Washington, D.C.: American Society for Microbiology.Google Scholar
Aserkoff, B. & Bennett, J. V. (1969). Effect of antibiotic therapy in acute salmonellosis on the fecal excretion of salmonellae. New England Journal of Medicine 281, 636–40.CrossRefGoogle ScholarPubMed
Bremner, D. A. (1978). The transfer of antibiotic resistance by R-plasmids in Shigellae. New Zealand Medical Journal 88, 9–10.Google ScholarPubMed
Chandler, M., Silver, L., Frey, J. & Caro, L. (1977). Suppression of an Escherichia coli dnaA mutation by the integrated R factor R100–1: generation of small plasmids after integration. Journal of Bacteriology 130, 303–11.CrossRefGoogle ScholarPubMed
Cohen, S. N. (1976). Transposable genetic elements and plasmid evolution. Nature, London 263, 731–8.CrossRefGoogle ScholarPubMed
Ericsson, H. M. & Sherris, J. C. (1971). Antibiotic sensitivity testing. Report of an international collaborative study. From Acta pathologica et microbiologica scandinavica 79, Section B, Supplement Number 217.Google Scholar
Mcdonell, M. W., Simon, M. N. & Studier, F. W. (1977). Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. Journal of Molecular Biology 110, 119–46.CrossRefGoogle ScholarPubMed
Meyers, J. A., Sanchez, D., Elwell, L. P. & Falkow, S. (1976). Simple agarose gel electrophoretic method for the identification and characterisation of plasmid deoxyribonucleic acid. Journal of Bacteriology 127, 1529–37.CrossRefGoogle ScholarPubMed
Tanaka, T. & Weisblum, B. (1975). Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. Journal of Bacteriology 121, 354–62.CrossRefGoogle ScholarPubMed
Thompson, R., Hughes, S. G. & Broda, P. (1974). Plasmid identification using specific endonucleases. Molecular and General Genetics 133, 141–9.CrossRefGoogle ScholarPubMed
Zasloff, M., Ginder, G. D. & Felsenfeld, G. (1978). A new method for the purification and identification of covalently closed circular DNA molecules. Nucleic Acids Research 5, 1139–52.CrossRefGoogle Scholar