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Clonal diffusion of EPEC-like Escherichia coli from rabbits as detected by ribotyping and random amplified polymorphic DNA assays

Published online by Cambridge University Press:  15 May 2009

S. Leroy-Setrin ,
M. C. Lesage ,
E. Chaslus-Dancla  and
J. P. Lafont
S. Leroy-Setrin
Affiliation:
Station de Pathologie Aviaire et de Parasitologie, Institut National de la Recherche Aqronomique, Centre de Tours, 37380 Nouzilly, France
M. C. Lesage
Affiliation:
Station de Pathologie Aviaire et de Parasitologie, Institut National de la Recherche Aqronomique, Centre de Tours, 37380 Nouzilly, France
E. Chaslus-Dancla*
Affiliation:
Station de Pathologie Aviaire et de Parasitologie, Institut National de la Recherche Aqronomique, Centre de Tours, 37380 Nouzilly, France
J. P. Lafont
Affiliation:
Station de Pathologie Aviaire et de Parasitologie, Institut National de la Recherche Aqronomique, Centre de Tours, 37380 Nouzilly, France
*
* Author for correspondence.
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Summary

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The genetic diversity and clonal relationships among 77 Escherichia coli strains isolated in France from diarrhoeic rabbits and that belonged to seven O serogroups including the predominant O103 serogroup, were estimated by ribotyping and random amplified polymorphic DNA (RAPD) assays. Fifteen ribotypes were defined. Most of the highly pathogenic O103 strains could be assigned to two major groups. Non-pathogenic strains were clearly distinguished. RAPD assays generally matched ribotyping, or gave more precision for subdividing strains from the two main O103 groups. The results on strains isolated from different areas and over a 9-year period showed the relevance of the association of these two methods for the survey of the spread of strains in breeding flocks and illustrated clonal diffusion in rabbit production structures.

Type
Special Article
Information
Epidemiology & Infection , Volume 114 , Issue 1 , February 1995 , pp. 113 - 121
Copyright
Copyright © Cambridge University Press 1995

References

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