Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-12T20:42:04.732Z Has data issue: false hasContentIssue false
  • English
  • Français

A comparison of confirmatory media for coliform organisms and Escherichia coli in water

Published online by Cambridge University Press:  25 March 2010

Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Gas production by coliform organisms and Escherichia coli from lauryl tryptose lactose broth (LTLB) was compared with that from brilliant green (lactose) bile broth (BGB). These media were compared with lauryl tryptose mannitol broth (LTMB) with and without added tryptophan for both gas and indole production. At 37 °C, LTLB and BGB were both satisfactory for gas production, but at 44 °C, LTLB gave fewer false-negative results and was thus significantly less inhibitory than BGB. However when LTLB and LTMB were compared as single-tube confirmatory media, LTLB give a high proportion of false-negative reactions in the indole test at 44 °C. The substitution of mannitol for lactose and the addition of tryptophan yielded a satisfactory medium for both confirmation of gas production and the demonstration of indole at 44 °C.

Type
Research Article
Information
Journal of Hygiene , Volume 87 , Issue 3 , December 1981 , pp. 369 - 375
Copyright
Copyright © Cambridge University Press 1981

References

Public Health Laboratory Service Standing Committee on the Bacteriological Examination of Water Supplies (1968). Confirmatory tests for coliform organisms. Journal of Hygiene 66, 641.Google Scholar
Public Health Laboratory Service and Standing Committee of Analysts (1980 a). A comparison between minerals-modified glutamate medium and lauryl tryptose broth for the enumeration of Escherichia coli and coliform organisms in water by the multiple tube method. Journal of Hygiene 85, 35.CrossRefGoogle Scholar
Public Health Laboratory Service and Standing Committee of Analysts (1980 b). Single tube confirmatory tests for Escheriehia coli. Journal of Hygiene 85, 51.CrossRefGoogle Scholar
Public Health Laboratory Service and Standing Committee of Analysts (1980 c). Membrane filtration media for the enumeration of coliform organisms and Eacherichia coli in water: a comparison of Tergitol 7 and lauryl sulphate with Teepol 610. Journal of Hygiene 85, 181.Google Scholar
Report (1969). The Bacteriological Examination of Water Supplies. Report No. 71 on Public Health and Medical Subjects. H.M.S.O., London.Google Scholar