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Detection of bacterial antigens by means of sensitized guinea-pig ileum

Published online by Cambridge University Press:  15 May 2009

F. E. Buckland
F. E. Buckland
Affiliation:
Microbiological Research Establishment, Porton, Salisbury, Wilts
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Using a Dale organ bath and the ileum of passively sensitized guinea-pigs a specific sensitive reaction with as little as 1 μg./ml. of disintegrated whole cultures of various bacteria and 0·01 μg./ml. of purified Sh. dysenteriae and S. typhimurium polysaccharides was obtained.

More detailed study showed that 1 μg./ml. of Sh. dysenteriae degraded polysaccharide derived from the O antigen could be detected with consistency. Lower concentrations gave irregular results.

My thanks are due to the David Bruce Laboratory for supplying the antisera and the bacterial suspensions, to Prof. W. T. J. Morgan of the Lister Institute of Preventive Medicine for the antibody nitrogen determinations, to Dr D. A. L. Davies of the Microbiological Research Establishment, Porton, for the antigen preparations and to Corporals G. Willis and P. Brown, R.A.F., for much careful work in day to day experiments.

Type
Research Article
Information
Journal of Hygiene , Volume 58 , Issue 3 , September 1960 , pp. 291 - 295
Copyright
Copyright © Cambridge University Press 1960

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