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Enzyme immunoassay of the antibody response to Brucella and Yersinia enterocolitica 09 infections in humans

Published online by Cambridge University Press:  25 March 2010

A. A. Lindberg ,
S. Hæggman ,
K. Karlson ,
H. E. Carlsson  and
N. S. Mair
A. A. Lindberg
Affiliation:
Department of Bacteriology, National Bacteriological Laboratory, S–105 21 Stockholm, Sweden
S. Hæggman
Affiliation:
Department of Bacteriology, National Bacteriological Laboratory, S–105 21 Stockholm, Sweden
K. Karlson
Affiliation:
Department of Bacteriology, National Bacteriological Laboratory, S–105 21 Stockholm, Sweden
H. E. Carlsson
Affiliation:
Department of Bacteriology, National Bacteriological Laboratory, S–105 21 Stockholm, Sweden
N. S. Mair
Affiliation:
Department of Microbiology, University of Leicester, Leicester, LE1 7RH
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Summary

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An enzyme immunoassay, with phenol–water-extracted lipopolysaccharide (LPS) from Brucella abortus as antigen, was used to detect the class-specific antibody response in sera from 173 patients with B. abortus, B. melitensis or B. suis infection. Sera from 30 patients with salmonellosis, yersiniosis or tularaemia and from 25 healthy individuals served as controls. The B. abortus LPS antigen permitted a safe diagnosis of acute and chronic brucellosis with high IgM and rising IgG titres in sera collected in the acute stage of the disease, and with elevated IgG titres only in the chronic stage. The B. abortus LPS antigen also permitted a specific diagnosis with the exception of the high titres estimated in sera from patients with Yersinia enterocolitica 09 infection. The problem with that well-known reciprocal cross-reactivity was overcome by using two additional antigens: Y. enterocolitica 09 native and periodate oxidized and borohydride reduced LPS preparations. In sera from patients with brucellosis high titres were estimated against all three antigens, whereas in sera from patients with yersiniosis caused by serotype 09 high titres were measurable only with the B. abortus and the Y. enterocolitica native LPS antigens. These data suggest that the B. abortus and Y. enterocolitica 09 LPS share one antigenic determinant resistant to periodate oxidation and borohydride reduction, and that in addition the Y. enterocolitica 09 LPS has a determinant which is sensitive to periodate oxidation and borohydride reduction.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 2 , April 1982 , pp. 295 - 307
Copyright
Copyright © Cambridge University Press 1982

References

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