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Factors influencing the establishment and spread of R-plasmids in an experimental model of urinary tract infection

Published online by Cambridge University Press:  25 March 2010

Laureen Snowsill ,
K. J. Towner  and
M. J. Lewis
Laureen Snowsill
Affiliation:
Department of Microbiology and Public Health Laboratory, University Hospital, Nottingham NG7 2UH
K. J. Towner
Affiliation:
Department of Microbiology and Public Health Laboratory, University Hospital, Nottingham NG7 2UH
M. J. Lewis
Affiliation:
Department of Microbiology and Public Health Laboratory, University Hospital, Nottingham NG7 2UH
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An experimental model simulating infection of the urinary tract was used to investigate factors influencing the transfer of plasmid-mediated drug resistance in the human bladder in the absence of antibiotic therapy. When a small number of cells carrying plasmid-mediated drug resistance was added to an established population of sensitive cells, it was found that varying the physical conditions of the bladder model had no significant effect on the spread of the plasmid and, under all conditions used, exponential spread of the plasmid through the originally sensitive population occurred. When the initial donor cells were killed shortly after addition to the model, exponential transfer of resistance amongst the established sensitive population still occurred. Thus, even in the absence of antibiotic therapy, only a small number of initial transfer events was required for a fully resistant population to be developed. This was true of both actively multiplying and early stationary phase cultures. An initially greater proportion of resistant cells, or the use of an antimicrobial agent to which some of the cells are resistant, would ensure that this process occurred more rapidly.

Type
Research Article
Information
Journal of Hygiene , Volume 87 , Issue 1 , August 1981 , pp. 83 - 91
Copyright
Copyright © Cambridge University Press 1981

References

Anderson, J. D. (1974). The effect of R factor carriage on the survival of Escherichia coli in the human intestine. Journal of Medical Microbiology 7, 85.CrossRefGoogle ScholarPubMed
Broda, P., Cullum, J. & Collins, J. F. (1977). In Plasmids – Medical and Theoretical Aspects, (ed. Mitsuhashi, S., Rosival, L. and Kréméry, V.), pp. 469–72. Berlin: Springer Verlag.Google Scholar
Chabbert, Y. A., Baudens, J. G. & Bouanchaud, D. M. (1969). In Bacterial Episomes and Plasmids, (ed. Wolstenholme, G. E. W. and O'Connor, M.), pp. 227–43. London: Churchill.Google Scholar
Coetzee, J. N., Datta, N. & Hedges, R. W. (1972). R factors from Proteus rettgeri. Journal of General Microbiology 72, 543.CrossRefGoogle ScholarPubMed
Cullum, J., Collins, J. F. & Broda, P. (1978 a). Factors affecting the kinetics of progeny formation with F'lac in Escherichia coli K12. Plasmid 1, 536.CrossRefGoogle ScholarPubMed
Cullum, J., Collins, J. F. & Broda, P. (1978 b). The spread of plasmids in model populations of Escherichia coli K12. Plasmid 1, 545.CrossRefGoogle ScholarPubMed
Dale, J. W. & Smith, J. T. (1979). The effect of a plasmid on the growth and survival of E. coli. Antonie van Leeuwenhoek Journal of Microbiology and Serology 45, 103.CrossRefGoogle ScholarPubMed
Datta, N. & Hedges, R. W. (1972). Trimethoprim resistance conferred by W plasmids in Enterobacteriaceae. Journal of General Microbiology 72, 349.CrossRefGoogle Scholar
Dennison, S. (1972). Naturally-occurring R-factor, de-repressed for pilus synthesis, belonging to the same compatibility group as the sex factor F of Escherichia coli K12. Journal of Bacteriology 109, 416.CrossRefGoogle Scholar
Falkow, S. (1975). Infectious Multiple Drug Resistance, pp. 40–47. London: Pion Ltd.Google Scholar
Gillespie, W. A. (1976). In Scientific Foundations of Urology, Vol. 1, (ed. Williams, D. I. and Chisholm, G. D.), pp. 197–200. London: Heinemann.Google Scholar
Greenwood, D. & O'Grady, F. (1978). An in vitro model of the urinary bladder. Journal of Antimicrobial Chemotherapy 4, 113.CrossRefGoogle Scholar
Lacey, R. W. (1975). Antibiotic resistance plasmids of Staphylococcus aureus and their clinical importance. Bacteriological Reviews 39, 1.CrossRefGoogle ScholarPubMed
Melling, J., Ellwood, D. C. & Robinson, A. (1977). Survival of R-factor-carrying Escherichia coli in mixed cultures in the chemostat. FEMS Microbiology Letters 2, 87.CrossRefGoogle Scholar
Miller, J. H. (1972). Experiments in Molecular Genetics, pp. 13–23. New York: Cold Spring Harbor Laboratory.Google Scholar
Shand, D. G., Mackenzie, J. C., Cattell, W. R. & Cato, J. (1968). Estimation of residual urine volume with 131I-hippuran. British Journal of Urology 40, 196.CrossRefGoogle Scholar
Shand, D. G., Nimmon, C. C., O'Grady, F. & Cattell, W. R. (1970). Relation between residual urine volume and response to treatment of urinary infection. Lancet i, 1305.CrossRefGoogle Scholar
Smith, H. W. (1969). Transfer of antibiotic resistance from animal and human strains of Escherichia coli to resident E. coli in the alimentary tract of man. Lancet i, 1174.CrossRefGoogle Scholar
Stocker, B. A. D., Smith, S. M. & Ozeki, H. (1963). High infectivity of Salmonella typhimurium newly infected by the Col I factor. Journal of General Microbiology 30, 201.CrossRefGoogle Scholar
Willetts, N. S. (1974). The kinetics of inhibition of F'lac transfer by R100 in Escherichia coli. Molecular and General Genetics 129, 123.CrossRefGoogle Scholar
Wouters, J. T. M., Rops, C. & Van Andel, J. G. (1978). R-plasmid persistence in Escherichia coli under various environmental conditions. Proceedings of the Society for General Microbiology 5, 61.Google Scholar