Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-22T23:05:37.920Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic marker studies of poliovirus: I. Natural variation

Published online by Cambridge University Press:  15 May 2009

Yvonne E. Cossart
Yvonne E. Cossart
Affiliation:
Virus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London, N. W. 9
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The properties of naturally occurring poliovirus strains of each serotype have been studied using three marker tests: the R.C.T.40 test, intratypic serodifferentiation and, for type 1 strains only, inhibition by dextran sulphate.

The results show that while most strains resemble the prototype strain of the same serotype, considerable natural variation exists, especially in the ability to grow at temperatures between 39° and 40° C.

No correlation has been found between the results of the tests on individual strains and it is concluded that no single test is sufficient to characterize a strain.

Type
Research Article
Information
Journal of Hygiene , Volume 65 , Issue 1 , March 1967 , pp. 67 - 76
Copyright
Copyright © Cambridge University Press 1967

References

Bengtssen, S., Philipson, L., Persson, H. & Laurent, J. (1964). The basis for the interaction between attenuated poliovirus and polyanions. Virology 24, 617.CrossRefGoogle Scholar
Benyish-Melnick, M. & Melnick, J. L. (1959). The use of in vitro markers and monkey neurovirulence test to follow genetic changes in attenuated viruses multiplying in the human alimentary tract. IInd Int. Conf. live Poliovirus Vaccines, Washington, 1959. (Sci. Publ. panam. Hlth Org. no. 44, p. 179.)Google Scholar
Cossart, Y. E. (1966). Marker studies of poliovirus. Nature, Lond. 211, 1432.CrossRefGoogle ScholarPubMed
Dubes, G. R. & Chapin, M. (1956). Gold adapted genetic variants of poliovirus. Science, N.Y. 124, 386.CrossRefGoogle Scholar
Dubes, G. R. & Chapin, M. (1958). Poliovirus mutants with altered response to cystine. J. gen. Microbiol. 18, 320.CrossRefGoogle ScholarPubMed
Goffe, A. P. (1955). Studies on the distribution of poliomyelitis viruses in England and Wales. Proc. R. Soc. Med. 48, 937.Google Scholar
Kanda, I. & Melnick, J. L. (1959). In vitro differentiation of virulent and attenuated polioviruses by their growth character on M.S. cells. J. exp. Med. 109, 9.CrossRefGoogle Scholar
Karzon, M. E., Pollock, B. F. & Barron, A. L. (1959). Phase variation in Echovirus type 6. Virology 9, 564.CrossRefGoogle Scholar
Kelly, S. (1962). A new tissue culture marker for poliovirus. Rep. N.Y. St. Dep. Hlth, 1962, p. 56.Google Scholar
Koprowski, H., Carp, R., Morton, J. W., Cohen, B. & Plotkin, S. (1960). The application of genetic markers to the development and control of live poliovirus vaccine. Int. Conf. Live Poliovirus Vaccines, Washington, 1960. (Sci. Publ. panam. Hlth Org. no. 50, p. 53.)Google Scholar
Lwoff, A. (1962). The thermosensitive critical event in the viral cycle. Cold Spring Harb. Symp. quant. Biol. 27, 159.CrossRefGoogle ScholarPubMed
McBride, W. D. (1959). Antigenic analysis of polioviruses by kinetic studies of serum neutralization. Virology 7, 45.CrossRefGoogle ScholarPubMed
Magrath, D. I., Boulger, L. R. & Hartley, F. G. (1964). Strains of poliovirus from individuals fed Sabin vaccine studied by in vitro markers. Proc. Xth Symposium Europ. Ass. Poliomyelitis and Allied Diseases, Warsaw. Unpublished.Google Scholar
Nakano, J. H. & Gelfand, H. M. (1962). The use of a modified Wecker technique for serodifferentiation of type 1 polioviruses related and unrelated to Sabin's vaccine strains. Am. J. Hyg. 75, 363.Google Scholar
Ramos-Alvabez, M. & Sabin, A. B. (1954). Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proc. Soc. exp. Biol. Med. 87, 655.CrossRefGoogle Scholar
Sabin, A. B. (1961). Reproductive capacity of polioviruses of diverse origins at various temperatures. Gustav Stern Symposium on Perspectives in Virology, vol. II, 90. Minneapolis: Burgess, 1961.Google Scholar
Sabin, A. B., Hennessen, W. A. & Winsser, J. (1954). Studies on variants of poliomyelitis virus. I. Experimental segregation and properties of avirulent variants of three immunological types. J. exp. Med. 99, 551.CrossRefGoogle Scholar
Sabin, A. B. & Lwoff, A. (1959). The relation between the reproductive capacity of polioviruses at different temperatures in tissue culture and monkey neurovirulence. Science N. Y. 129, 1287.Google Scholar
Stanley, N. F., Dorman, D. C., Ponsford, J. & Larkin, M. (1956). Variants of poliovirus hominis. V. Isolation of heat resistant mutants. Aust. J. exp. Biol. med. Sci. 34, 411.CrossRefGoogle Scholar
Takemoto, K. K. & Kirschstein, R. L. (1964). Dextran sulfate plaque variants of attenuated type 1 poliovirus: relationship to in vivo and in vivo markers. J. Immun. 92, 329.CrossRefGoogle ScholarPubMed
Wallis, C., Melnick, J. L., Terry, G. D. & Wimberley, J. L. (1962). An aluminium marker for differentiation and separation of virulent and attenuated polioviruses. J. exp. Med. 115, 763.CrossRefGoogle ScholarPubMed
Wecker, E. (1960). A simple test for serodifferentiation of poliovirus strains within the same type. Virology 10, 376.CrossRefGoogle ScholarPubMed
Wenner, H. A., Archetti, J. & Dubes, G. (1959). Antigenic variations among type 1 polioviruses. A study of six wild type strains and five variants. Am. J. Hyg. 70, 66.Google Scholar