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Growth and enterotoxin production of Staphylococcus aureus in shrimp

Published online by Cambridge University Press:  19 October 2009

H. J. Beckers ,
F. M. van Leusden  and
P. D. Tips
H. J. Beckers
Affiliation:
Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Hygiëne, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands
F. M. van Leusden
Affiliation:
Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Hygiëne, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands
P. D. Tips
Affiliation:
Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Hygiëne, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands
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Strains of Staphylococcus aureus isolated from shrimp were examined for phage pattern and enterotoxin production; 63% of the strains isolated from North Sea shrimp were typable with the International and additional set of phages, as were 38% of the strains isolated from South-East Asian shrimp. Staphylococcal enterotoxin(s) (SE) were produced by 48% and 35% of strains isolated from North Sea and South-East Asian shrimp respectively. Growth and enterotoxin production by S. aureus in shrimp was examined in storage experiments at 22 °C. S. aureus increased by 1–2 log units in 24 h when the organism was only a minor part of the total microflora of shrimp. When S. aureus was an equivalent part of the total flora its numbers increased by 3–4 log units in 24 h. Enterotoxins A and B became detectable when the number of S. aureus exceeded 107 per g in aseptically peeled shrimp. Results indicate that S. aureus is able to produce enterotoxin in shrimp, but its production depends upon a number of factors, including the relationship between S. aureus and competitive micro-organisms. It is concluded that the presence of S. aureus on commercially produced shrimp represents a potential hazard to health.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 3 , December 1985 , pp. 685 - 693
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Anonymous (1978). International Organization for Standardization; Microbiology – General guidance for enumeration of micro-organism colony count technique at 30 °C. ISO·4833.Google Scholar
Anonymous (1979). International Organization for Standardization. Meat and meat products – Detection and enumeration of Enterobacteriaceae (Reference methods). ISO·5552.Google Scholar
Anonymous (1983). International Organization for Standardization; Microbiology – General guidance for the preparation of dilutions for microbiological examination. ISO·6887.Google Scholar
Beckers, H. J., van Leusden, F. M., Bindschedler, O. & Guerraz, D. (1984). Evaluation of a pour plate system with a rabbit plasma-bovine fibrinogen agar for the enumeration of Staphylococcus aureus in food. Canadian Journal of Microbiology 30, 470–474.CrossRefGoogle ScholarPubMed
Beckers, H. J., van Schothorst, M., van Spreekens, K. J. A. & Oosterhuis, J. J. (1981). Microbiological quality of frozen precooked and peeled shrimp from South East Asia and from the North Sea. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionkrankheiten und Hygiene (I. Abt. Orig. B) 172, 401–410.Google ScholarPubMed
Bergdoll, M. S., Crass, B. A., Reiser, R. P., Robbins, R. N. & Savis, J. P. (1981). A new staphylococcal enterotoxin, enterotoxin F, associated with toxic-shock-syndrome Staphylococcus aureus isolates. Lancet i, 1017–1021.CrossRefGoogle Scholar
Bühning-Pfaue, H., Timmermans, P. & Notermans, S. (1981). Einfache Methode für der Nachweis von Staphylokokken-Enterotoxin-B in Vanillepudding mittels ELISA-Test. Zeitschrift für Lebensmitteluntersuchung und Forschung 173, 351–355.CrossRefGoogle Scholar
De Nooy, M. P., van Leeuwen, W. J. & Notermans, S. (1982). Enterotoxin production by strains of Staphylococcus aureus isolated from clinical and non-clinical specimens with special reference to enterotoxin F and toxic shock syndrome. Journal of Hygiene 89, 499–505.CrossRefGoogle Scholar
Donnelly, C. B., Leslie, J. E., Black, L. A. & Lewis, K. H. (1967). Serological identification of enterotoxigenic staphylococci from cheese. Applied Microbiology 15, 1381–1387.CrossRefGoogle ScholarPubMed
Food and Agriculture Organization of the United Nations/World Health Organization (1977). Report of the Second joint FAO/WHO expert consultation on microbiological specifications for foods. EC/Microbiol./77/Report 2; Rome.Google Scholar
Gilbert, R. J. (1982). The microbiology of some foods imported into England through the Port of London and Heathrow (London) Airport. In Control of the Microbial Contamination of Foods and Feeds in International Trade: Microbial Standards and Specifications (ed. Kurata, H. and Hesseltine, C. W.), pp. 105–119. Tokyo: Saikon.Google Scholar
Gilbert, R. J. & Wieneke, A. A. (1973). Staphylococcal food poisoning with special reference to the detection of enterotoxin in food. In The Microbiological Safety of Food (ed. Hobbs, B. C. and Christian, J. H. B.), pp. 273–285. London: Academic Press.Google Scholar
Notermans, S., Boot, R., Tips, P. D. & de Nooy, M. P. (1983). Extraction of Staphylococcal enterotoxin (SE) from minced meat and subsequent detection of SE with enzyme linked immunosorbent assay (ELISA). Journal of Food Protection 46, 238–241.CrossRefGoogle ScholarPubMed
Olsvik, Ø., Berdal, B. P., Fossum, K & Omland, T. (1981). Enterotoxin production by Staphylococcus aureus related to the origin of the strains. Acta Pathologica et Microbiologica Scandinavica B 89, 423–426.Google Scholar
Petrás, P. & Maskova, L. (1982). Detection of staphylococcal enterotoxigenicity. III. Strains isolated in 1979–1980. Journal of Hygiene, Epidemiology, Microbiology and Immunology 26, 95–101.Google ScholarPubMed
Report Subcommittee Staphylococcal Phage-typing (1975). International Journal of Systematic Bacteriology 25, 241–242.CrossRefGoogle Scholar
Robbins, R., Gould, S. & Bergdoll, M. S. (1974). Detecting the enterotoxigenicity of Staphylococcus aureus strains. Applied Microbiology 28, 946–950.CrossRefGoogle ScholarPubMed
Todd, E., Park, C., Holmes, R., Chaundhry, S., Emson, H. & Link, H. (1973). Suspected staphylococcal food poisoning from ham and shrimp obtained from maltreated cans. Canadian Journal of Public Health 64, 360–363.Google ScholarPubMed
Turnbull, P. C. B. & Gilbert, R. J. (1982). Fish and shellfish poisoning in Britain. In Adverse Effects of Foods (ed. Jelliffe, E. F. P. and Jelliffe, D. B.), pp. 297–306. New York: Plenum Press.CrossRefGoogle Scholar
Van Leeuwen, W. J. & Rost, J. A. (1976). An additional set of phage for the typing of Staphylococcus aureus strains of human origin and non-typable with the international basic set of phages. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (I. Abt. Supplement Heft 5), 1013–1019.Google Scholar
Wieneke, A. A. (1974). Enterotoxin production by strains of Staphylococcus aureus isolated from foods and human beings. Journal of Hygiene 73, 255–261.CrossRefGoogle ScholarPubMed