Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-22T21:55:44.308Z Has data issue: false hasContentIssue false
  • English
  • Français

Lack of association of Escherichia coli exhibiting both mannose-resistant haemagglutination and diffuse adherence to Hep-2 cells with acute diarrhoea in children

Published online by Cambridge University Press:  15 May 2009

M. Čobeljić ,
I. Stojiljković ,
A. Budišin ,
Z. Lepšanović  and
N Lerš
M. Čobeljić
Affiliation:
Institute of Preventive Medicine, Military Medical Academy, Crnotravska 17, 11000 Belgrade, Yugoslavia
I. Stojiljković
Affiliation:
‘Rudjer Bošković’ Institute, 41000 Zagreb, Yugoslavia
A. Budišin
Affiliation:
Institute of Preventive Medicine, Military Medical Academy, Crnotravska 17, 11000 Belgrade, Yugoslavia
Z. Lepšanović
Affiliation:
Institute of Preventive Medicine, Military Medical Academy, Crnotravska 17, 11000 Belgrade, Yugoslavia
N Lerš
Affiliation:
‘Rudjer Bošković’ Institute, 41000 Zagreb, Yugoslavia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Stool specimens from 631 children with acute diarrhoea and from 277 healthy controls were tested for the presence of non-enteropathogenic, non-enterotoxigenic Escherichia coli strains which mediated mannose-resistant haemagglutination of human erythrocytes (MRHA + ). Fifty-nine (34·9%) of 169 isolated MRHA + strains but none of 210 MRHA - strains exhibited diffuse adherence (DA+) to HEp–2 cells. DA+ strains were found in 37 (5·9%) children with diarrhoea and in 22 (7·9%) controls. MRHA + /DA + strains in comparison to MRHA + /DA - strains significantly less frequently expressed P fimbriae (10·7 vs. 73·6%), haemolysin production (12·5 vs. 63·2%), and MRHA of other species erythrocytes (21·4 vs. 84%).

These data demonstrate that E. coli which exhibit the diffuse pattern of adherence to HEp–2 cells also cause MRHA of human erythrocytes. Since these strains were found with similar frequencies in children with and without diarrhoea it seems that DA is not a marker of enteropathogenicity of E. coli.

Type
Research Article
Information
Epidemiology & Infection , Volume 104 , Issue 1 , February 1990 , pp. 127 - 133
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

1.Archambaud, M, Courcoux, P, Ouin, V, Chabanon, G, Labigne-Roussel, A.Phenotypic and genotypic assays for the detection and identification of adhesins from pyelonephritic Escherichia coli. Ann Inst Pasteur/Microbiol 1988; 139: 557–73.CrossRefGoogle ScholarPubMed
2.Archambaud, M, Courcoux, P, Labigne–Roussel, A.Detection by molecular hybridization of pap, afa, and sfa adherence systems in Escherichia coli strains associated with urinary and enteral infections. Ann Inst Pasteur/Microbiol 1988; 139: 575–88.CrossRefGoogle ScholarPubMed
3.Evans, DJ, Evans, DG, DuPont, HL.Hemagglutination patterns of enterotoxigenic Escherichia coli determined with human, bovine, chicken, and guinea–pig erythrocytes in the presence and absence of mannose. Infect Immun 1979; 23: 336–46.CrossRefGoogle ScholarPubMed
4.Scaletsky, ICA, Silva, MLM, Toledo, MRF, Davis, BR, Blake, PA, Trabulsi, LR.Correlation between adherence to HeLa cells serogroups serotypes, bioserotypes of Escherichia coli. Infect Immun 1985; 49: 528–32.CrossRefGoogle ScholarPubMed
5.Nataro, JP, Kaper, JB, Robins-Browne, R, Prado, V, Vial, P.Levine, MM.Patterns of adherence of diarrheagenic Escherichia coli to HEp–2 cells. Pediatr Infect Dis J 1987; 6: 829–31.CrossRefGoogle ScholarPubMed
6.Mathewson, JJ, Johnson, PC, DuPont, HL, et al. A newly recognized cause of travellers diarrhea: enteroadherent Escherichia coli. J Infect Dis 1985; 151: 471–5CrossRefGoogle ScholarPubMed
7.Čobeljić, M, Mel, D, Arsić, B, et al. The association of enterotoxigenic and enteropathogenic Escherichia coli and other enteric pathogens with childhood diarrhoea in Yugoslavia. Epidemiol Infect 1989; 103: 53–62.CrossRefGoogle ScholarPubMed
8.Nataro, JP, Scaletsky, ICA, Kaper, JB, Levine, MM, Trabulsi, LR, Plasmid–mediated factors conferring diffuse localized adherence of enteropathogenic Escherichia coli. Infect Immun 1985; 48: 378–83.CrossRefGoogle ScholarPubMed
9.Lindahl, M, Paris, A, Wadstrom, T, Hjerten, S.A new test based on ‘salting out’ to measure relative surface hydrophobicity of bacterial cells. Biochim Biophys Acta 1981; 677: 471–6.CrossRefGoogle ScholarPubMed
10.Frederiq, P.Actions antibiotiques reciproques chez les Enterobacteriaceae. Rev Beige Pathol Med Exper 1948; 19: (suppl. IV), 1.Google Scholar
11.Payne, SM, Niesel, DW, Peixotipo, SS, Lawlor, KM.Expression of hydroxamate and phenolate siderophores by Shigella flexneri. J Bacteriol 1983; 155: 949–55.CrossRefGoogle ScholarPubMed
12.Väisänen, V, Elo, J, Tallgren, LG, et al. Mannose–resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis. Lancet 1981; ii: 1366–9.CrossRefGoogle Scholar
13.Leffler, H, Svanborg–Eden, C.Glycolipid receptors for uropathogenic Escherichia coli on human erythrocytes and uroepithelial cells. Infect Immun 1981; 34: 920–9.CrossRefGoogle ScholarPubMed
14.Nandadasa, HG, Sargent, GF, Brown, MGM, McNeish, AS, Williams, PH.The role of plasmids in adherence of invasive Escherichia coli to mammalian cells. J Infect Dis 1981; 143: 286–90.CrossRefGoogle ScholarPubMed
15.Williams, PH, Knutton, S, Brown, MGM, Candy, DCA, McNeish, AS.Characterization of nonfimbrial mannose–resistant protein hemagglutinins of two Escherichia coli strains isolated from infants with enteritis. Infect Immun 1984; 44: 592–8.CrossRefGoogle ScholarPubMed
16.Tavendale, A, Old, DC.Haemagglutinins and adhesion of Escherichia coli to HEp–2 epithelial cells. J Med Microbiol 1985; 158: 70–9.Google Scholar
17.Nicoletti, M, Superti, F, Conti, C, Calconi, A, Zagaglia, C.Virulence factors of lactose–negative Escherichia coli strains isolated from children with diarrhea in Somalia. J Clin Microbiol 1988; 26: 524–9.CrossRefGoogle ScholarPubMed
18.Sherman, P, Drumm, B, Karmali, M, Cutz, E.Adherence of bacteria to the intestine in sporadic cases of enteropathogenic Escherichia coli-associated diarrhea in infants and young children: a prospective study. Gastroenterology 1989; 96: 86–94.CrossRefGoogle Scholar
19.Benz, I, Schmidt, A.Cloning and expression of an adhesin (AIDA-I) involved in diffuse adherence of enteropathogenic Escherichia coli. Infect Immun 1989; 57: 1506–11.CrossRefGoogle ScholarPubMed
20.Vial, P, Robins-Browne, R, Lior, H, et al. Characterization of enteroadherent-aggregative Escherichia coli, a putative agent of diarrheal disease. J Infect Dis 1988; 158: 70–9.CrossRefGoogle ScholarPubMed
21.Johnson, JR, Moseley, SL, Roberts, PL, Stamm, WE.Aerobactin and other virulence factor gene among strains of Escherichia coli causing urosepsis: association with patient characteristics. Infect Immun 1988; 56: 405–12.CrossRefGoogle ScholarPubMed
22.O'Hanley, P, Low, D, Romero, I, et al. Gal–Gal binding and hemolysin phenotypes and genotypes associated with uropathogenic Escherichia coli. N Engl J Med 1985; 313: 414–20.CrossRefGoogle ScholarPubMed
23.Low, D, David, V, Lark, D, Schoolnik, G, Falkow, S.Gene clusters governing the production of hemolysin and mannose–resistant hemagglutination are closely linked in Escherichia coli serotype 0 4 and O 6 isolated from urinary tract infections. Infect Immun 1984; 43: 353–8.CrossRefGoogle Scholar
24.Jacobson, SH, Hammarlind, M, Lidelfeldt, KJ, Osterberg, E, Tullus, K, Bruner, A. Incidence of aerobactin-positive Escherichia coli strains in patients with symptomatic urinary tract infection. Eur J Clin Microbiol Infect Dis 1988; 7: 630–1.CrossRefGoogle Scholar
25.Bilge, S, Clausen, CR, Lau, W, Moseley, SL.Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp–2 cells. J Bacteriol 1989; 171: 4281–89.CrossRefGoogle ScholarPubMed
26.Mathewson, JJ, Oberhelman, RA, DuPont, HL, Cabada, FJ, Garibay, EV.Enteroadherent Escherichia coli as a cause of diarrhea among children in Mexico. J Clin Microbiol 1987; 25: 1917–19.CrossRefGoogle ScholarPubMed
27.Chatkaeomorakot, A, Echeverria, P, Taylor, DN, et al. HeLa cell–adherent Escherichia coli in children with diarrhea in Thailand. J Infect Dis 1987; 156: 669–72.CrossRefGoogle ScholarPubMed
28.Levine, MM, Prado, V, Robins–Browne, R, et al. , Use of DNA probes and HEp–2 cell adherence assay to detect diarrheagenic Escherichia coli. J Infect Dis 1988; 158: 70–9.CrossRefGoogle ScholarPubMed
29.Cravioto, A, Reyes, RE, Ortega, R, Fernandez, G, Hernandez, R, Lopez, D.Prospective study of diarrhoeal disease in a cohort of rural Mexican children: incidence and isolated pathogens during the first two years of life. Epidem Inf 1988; 101: 123–34.CrossRefGoogle Scholar
30.Gomes, TAT, Blake, PA, Trabulsi, LR.Prevalence of Escherichia coli strains with localized, diffuse, and aggregative adherence to HeLa cells in infants with diarrhea and matched controls. J Clin Microbiol 1989; 27: 266–9.CrossRefGoogle ScholarPubMed