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Properties of a cell-wall-defective variant of Brucella abortus of bovine origin

Published online by Cambridge University Press:  15 May 2009

M. J. Corbel ,
A. C. Scott  and
H. M. Ross
M. J. Corbel
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, New Haw, Weybridge, Surrey
A. C. Scott
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, New Haw, Weybridge, Surrey
H. M. Ross
Affiliation:
The North of Scotland College of Agriculture, Veterinary Investigation Centre, Drummondhill, Stratherrick Road, Inverness, IV2 4JZ
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The properties of an atypical Brucella strain isolated from lymph node tissue of a cow slaughtered as a brucellosis reactor were examined. The organism was Gram negative and highly pleomorphic, existing as cocci, coccobacilli, rods, branched and irregular forms which stained with fluorescent antibody conjugates prepared against rough and smooth Brucella abortus strains. It produced lecithinase and required at least 15% v/v equine or bovine serum for growth. It did not need supplementary CO2 for growth, produced H2S and was inhibited by brucella dyes and partially by i-erythritol. Growth inhibition or lysis was produced by brucellaphages. The organism was not pathogenic for guinea-pigs or mice but evoked antibodies mainly to rough Brucella antigens.

Type
Research Article
Information
Journal of Hygiene , Volume 85 , Issue 1 , August 1980 , pp. 103 - 113
Copyright
Copyright © Cambridge University Press 1980

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