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Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis

Published online by Cambridge University Press:  15 May 2009

R. J. Chappel ,
P. Williamson ,
D. J. McNaught ,
M. J. Dalling  and
G. S. Allan
R. J. Chappel
Affiliation:
‘Attwood’ Veterinary Research Laboratory, Victorian Department of Agriculture, TVestmeadows, Victoria, 3047, Australia
P. Williamson
Affiliation:
‘Attwood’ Veterinary Research Laboratory, Victorian Department of Agriculture, TVestmeadows, Victoria, 3047, Australia
D. J. McNaught
Affiliation:
‘Attwood’ Veterinary Research Laboratory, Victorian Department of Agriculture, TVestmeadows, Victoria, 3047, Australia
M. J. Dalling
Affiliation:
‘Attwood’ Veterinary Research Laboratory, Victorian Department of Agriculture, TVestmeadows, Victoria, 3047, Australia
G. S. Allan
Affiliation:
‘Attwood’ Veterinary Research Laboratory, Victorian Department of Agriculture, TVestmeadows, Victoria, 3047, Australia
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Summary

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A radioimmunoassay (RIA) has been developed to measure antibodies against Brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. The RIA measures the amount of specific antibody of the IgG1 and IgG2 subclasses but is insensitive to 1gM, a characteristic which may make it more suitable than the complement fixation test (OFT) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with Br. abortus strain 19. The RIA is not subject to prozoning or ambiguous reactions, both of which interfere with the interpretation of the CFT.

Type
Research Article
Information
Journal of Hygiene , Volume 77 , Issue 3 , December 1976 , pp. 369 - 376
Copyright
Copyright © Cambridge University Press 1976

References

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