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RAPD analysis of isolates of Burkholderia pseudomallei from patients with recurrent melioidosis

Published online by Cambridge University Press:  15 May 2009

A. Haase ,
A. Melder ,
H. Smith-Vaughan ,
D. Kemp  and
B. Currie
A. Haase
Affiliation:
Menzies School of Health Research, P.O. Box 41096, Casuarina NT 0811, Australia
A. Melder
Affiliation:
Menzies School of Health Research, P.O. Box 41096, Casuarina NT 0811, Australia
H. Smith-Vaughan
Affiliation:
Menzies School of Health Research, P.O. Box 41096, Casuarina NT 0811, Australia
D. Kemp
Affiliation:
Menzies School of Health Research, P.O. Box 41096, Casuarina NT 0811, Australia
B. Currie
Affiliation:
Menzies School of Health Research, P.O. Box 41096, Casuarina NT 0811, Australia
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Summary

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Twenty-seven isolates of Burholderia pseudomallei (formerly Pseudomonas pseudomallei) from ten patients with recurrent melioidosis were analysed by RAPD. In two cases RAPD patterns in recurrent isolates differed from the original isolates; one was considered a likely reinfection while the other may represent relapse from one of two strains initially infecting the patient. In two cases where a change in antibiotic resistance had occurred between original and relapse isolates, slight changes in RAPD patterns were found with one of the four primers used. In the other six cases the relapse was clearly due to the original strain re-emerging unchanged, with identical RAPD patterns with all four primers.

Type
Research Article
Information
Epidemiology & Infection , Volume 115 , Issue 1 , August 1995 , pp. 115 - 121
Copyright
Copyright © Cambridge University Press 1995

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