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Red-cell IgM-antibody capture assay for the detection of Mycoplasma pneumoniae-specific IgM

Published online by Cambridge University Press:  19 October 2009

R. R. A. Coombs ,
Gwen Easter ,
P. Matejtschuk  and
T. G. Wreghitt
R. R. A. Coombs
Affiliation:
Division of Immunology, Laboratories Block, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ
Gwen Easter
Affiliation:
Division of Immunology, Laboratories Block, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ
P. Matejtschuk
Affiliation:
Division of Immunology, Laboratories Block, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ
T. G. Wreghitt
Affiliation:
Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ
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Summary

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A red-cell IgM-antibody capture assay has been developed for detecting Mycoplasma pneumoniae-specific IgM, which is based on the adsorption or ‘capture’ of IgM from patients' sera onto so-called ‘inagglutinable’ bovine red cells, chemically linked with anti-human μ When M. pneumoniae antigen is added to the system, the red cells agglutinate in the presence of M. pneumoniae-specific IgM.

The test was compared with the μ-capture ELISA described by Wreghitt & Sillis (1985), and was found to give comparable results. The two tests had similar sensitivity and specificity and could detect M. pneumoniae-spcific IgM for a similar time (up to 6 months) after proven M. pneumoniae infection.

However, the red-cell antibody capture assay is a much more simple and rapid test, taking only 1 h to perform (compared to 24 h for μ-capture ELISA). The redcell IgM-antibody capture assay is therefore amenable to rapid diagnosis of M. pneumoniae infection and the institution of early appropriate antibiotic therapy.

Type
Research Article
Information
Epidemiology & Infection , Volume 100 , Issue 1 , February 1988 , pp. 101 - 109
Copyright
Copyright © Cambridge University Press 1988

References

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