Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-19T16:32:16.664Z Has data issue: false hasContentIssue false
  • English
  • Français

The relative effectiveness of commonly used disinfectants in inactivation of coxsackievirus B5

Published online by Cambridge University Press:  15 May 2009

Murray Drulak ,
Alfred M. Wallbank ,
Ingrid Lebtag ,
Larry Werboski  and
Linda Poffenroth
Murray Drulak
Affiliation:
The Department of Medical Microbiology and Infectious Diseases, University of Manitoba Medical College, Winnipeg, Manitoba, CanadaR3E 0W3
Alfred M. Wallbank
Affiliation:
The Department of Medical Microbiology and Infectious Diseases, University of Manitoba Medical College, Winnipeg, Manitoba, CanadaR3E 0W3
Ingrid Lebtag
Affiliation:
The Department of Medical Microbiology and Infectious Diseases, University of Manitoba Medical College, Winnipeg, Manitoba, CanadaR3E 0W3
Larry Werboski
Affiliation:
The Department of Medical Microbiology and Infectious Diseases, University of Manitoba Medical College, Winnipeg, Manitoba, CanadaR3E 0W3
Linda Poffenroth
Affiliation:
The Department of Medical Microbiology and Infectious Diseases, University of Manitoba Medical College, Winnipeg, Manitoba, CanadaR3E 0W3
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Coxsackievirus B5 in the presence of fetal calf serum was exposed to six commonly used disinfectants for times of 10, 20 and 30 s. At the end of exposure times skim milk neutralized the disinfectant activity, with residual virus assayed by the plaque technique. The six disinfectants considered were Javex, sodium hydroxide, ethanol, Wescodyne, One Stroke Ves-Phene and Sonacide. Although 95% (v/v) ethanol was significantly more virucidal than dilutions of the other five disinfectants tested causing a 106 reduction in 20 s, it may not be practical to use in many instances. Next to 95% (v/v) ethanol, 1/75 (800 parts/b6) Javex, 0·25% (w7/v) sodium hydroxide and 1/200 Wescodyne were the most effective virucides. These disinfectants were equal in effectiveness causing a 105 reduction of coxsackievirus B5 in 30 s. Of these three disinfectants Javex is the most practical to use since sodium hydroxide is caustic and Wescodyne is selective in its virucidal action. Undiluted Sonacide was a less effective virucide causing a less than 10-fold reduction of coxsackievirus B5 in 30 s. A 1/50 dilution of One Stroke Ves-Phene was the least effective virucide tested since it did not significantly inactivate coxsackievirus B5 in 30 s.

Type
Research Article
Information
Journal of Hygiene , Volume 81 , Issue 3 , December 1978 , pp. 389 - 397
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Drulak, M., Wallbank, A. M. & Lebtag, I. (1978). The relative effectiveness of commonly used disinfectants in inactivation of echovirus 11. Journal of Hygiene 81, 77–87.CrossRefGoogle ScholarPubMed
Klein, M. (1956). The evaluation of the virucidal activity of germicides. Proceedings of the Chemical Specialities Manufacturers Association, May, pp. 98–100.Google Scholar
Klein, M. & Deforest, A. (1963). Antiviral action of germicides. Soap and Chemical Specialities 39, 70–2.Google Scholar
Noll, H. & Youngner, J. S. (1959). Virus–lipid interactions. II. The mechanism of absorption of lipophilic viruses to water insoluble polar lipids. Virology 8, 319–43.Google Scholar
Saitanu, K. & Lund, E. (1975). Inactivation of enterovirus by glutaraldehyde. Applied Microbiology 29, 571–4.CrossRefGoogle ScholarPubMed
Wallis, C., Behbelami, A. M., Lee, L. H. & Bianchi, M. (1963). The ineffectiveness of organic iodine (Wescodyne) as a viral disinfectant. American Journal of Hygiene 78, 325–9.Google ScholarPubMed
West Chemical Products Inc. Wescodyne. Detergent-Germicide for hospital use containing ‘Tamed Iodine’, West Chemical Products Inc. 42–16 West Street, Long Island City, N.Y. 11101.Google Scholar