Hostname: page-component-848d4c4894-5nwft Total loading time: 0 Render date: 2024-06-09T16:47:23.456Z Has data issue: false hasContentIssue false
  • English
  • Français

Restriction fragment length polymorphism of the pMJ101-like plasmid and ribotyping in the fish pathogen Vibrio ordalii

Published online by Cambridge University Press:  15 May 2009

K. Pedersen ,
S. Koblavi ,
T. Tiainen  and
P. A. D. Grimont
K. Pedersen
Affiliation:
Royal Veterinary and Agricultural University, Department of Veterinary Microbiology, Laboratory of Fish Diseases, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark,
S. Koblavi
Affiliation:
Centre National de Typage Moléculaire Entérique, Unité des Entérobactéries, Unité INSERM 399, Institut Pasteur, 28 rue du Docteur Roux, F-75724 Paris Cedex 15, France
T. Tiainen
Affiliation:
Royal Veterinary and Agricultural University, Department of Veterinary Microbiology, Laboratory of Fish Diseases, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark,
P. A. D. Grimont
Affiliation:
Centre National de Typage Moléculaire Entérique, Unité des Entérobactéries, Unité INSERM 399, Institut Pasteur, 28 rue du Docteur Roux, F-75724 Paris Cedex 15, France
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A total of 32 Vibrio ordalii strains were studied for their plasmid content and shown to carry a plasmid of approximately 32 kb. This plasmid was subsequently subjected to restriction fragment length polymorphism (RFLP) studies. Using Hind III, three different restriction patterns were identified while BamHI cleaved the plasmid into a single linear fragment. The results suggest that the 32 kb plasmid is highly conserved but that some variation in restriction pattern occurs. The same set of strains was subjected to ribotyping. Using Mlu I, six different restriction patterns were demonstrated. Strains from the USA and Canada shared profiles with strains from Australia and Japan. Strains from Australia generated a single pattern whereas strains from North America were subdivided into three patterns, and the Japanese strains fell into five patterns. The results suggest that ribotyping in combination with RFLP studies of the pMJ101-like plasmid may be useful in epidemiological studies of V. ordalii.

Type
Research Article
Information
Epidemiology & Infection , Volume 117 , Issue 2 , October 1996 , pp. 385 - 391
Copyright
Copyright © Cambridge University Press 1996

References

Referances

1.Schiewe, MH, Crosa, JH. Molecular characterization of Vibrio anguillarum biotype 2. Can J Microbiol 1981; 27: 1011–8.CrossRefGoogle Scholar
2.Muroga, K, Jo, Y, Masumura, K. Vibrio ordalii isolated from diseased ayu (Plecoglossus altivelis) and rockfish (Sebastes schlegeli). Fish Pathol 1986; 21: 239–43.CrossRefGoogle Scholar
3.Wards, BJ, Patel, HH, Anderson, CD, de Lisle, GW. Characterization by restriction endonuclease analysis and plasmid profiling of Vibrio ordalii strains from salmon (Oncorhynchus tshawytscha and Oncorhynchus nerka) with vibriosis in New Zealand. New Zealand J Marine Freshwater Res 1991; 25: 345–50.CrossRefGoogle Scholar
4.Harrell, LW, Novotny, AJ, Schiewe, MH, Hodgins, HO. Isolation and description of two vibrios pathogenic to Pacific salmon in Puget Sound, Washington. Fisheries Bull 1976; 74: 447–9.Google Scholar
5.Schiewe, MH, Trust, TJ, Crosa, JH. Vibrio ordalii sp. nov.: a causative agent of vibriosis in fish. Curr Microbiol 1981: 6: 343–8.CrossRefGoogle Scholar
6.Schiewe, MH, Crosa, JH, Ordal, EJ. Deoxyribonucleic acid relationships among marine vibrios pathogenic to fish. Can J Microbiol 1977; 23: 954–8.CrossRefGoogle ScholarPubMed
7.Ransom, DP, Lannan, CN, Rohovec, JS, Fryer, JL. Comparison of histopathology caused by Vibrio anguillarum and Vibrio ordalii in three species of Pacific salmon. J Fish Dis 1984; 7: 107–15.CrossRefGoogle Scholar
8.Tiainen, T, Pedersen, K, Larsen, JL. Ribotyping and plasmid profiling of Vibrio anguillarum serovar O2 and Vibrio ordalii. J Appl Bacteriol 1995; 79: 384–92.CrossRefGoogle ScholarPubMed
9.Larsen, JL, Pedersen, K, Dalsgaard, I. Vibrio anguillarum serovars associated with vibriosis in fish. J Fish Dis 1994; 17: 259–67.CrossRefGoogle Scholar
10.Mutharia, LM, Amor, PA. Monoclonal antibodies against Vibrio anguillarum O2 and Vibrio ordalii identify antigenic differences in lipopolysaccharide O-antigens. FEMS Microbiol Lett 1994; 123: 289–98.CrossRefGoogle ScholarPubMed
11.Mutharia, LM, Raymond, BT, Dekievit, TR, Stevenson, RMW. Antibody specificities of polyclonal rabbit and rainbow trout antisera against Vibrio ordalii and serotype O:2 strains of Vibrio anguillarum. Can J Microbiol 1993; 39: 492–9.CrossRefGoogle Scholar
12.Bidinost, C, Crosa, JH, Actis, LA. Localization of the replication region of the pMJ101 plasmid from Vibrio ordalii. Plasmid 1994; 31: 242–50.CrossRefGoogle ScholarPubMed
13.Larsen, JL, Olsen, JE. Occurrence of plasmids in Danish isolates of Vibrio anguillarum serovars O1 and O2 and association with phenotypic characteristics. Appl Environ Microbiol 1991; 57: 2158–63.CrossRefGoogle ScholarPubMed
14.Olsen, JE, Larsen, JL. Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid. Appl Environ Microbiol 1990; 56: 3130–2.CrossRefGoogle ScholarPubMed
15.Grimont, F, Grimont, PAD. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur/Microbiol 1986; 137B: 165–75.CrossRefGoogle ScholarPubMed
16.Koblavi, S, Grimont, F, Grimont, PAD. Clinical diversity of Vibrio cholerae O1 evidenced by rRNA gene restriction patterns. Res Microbiol 1990; 141: 645–57.CrossRefGoogle Scholar
17.Aznar, R, Ludwig, W, Schleifer, KH. Ribotyping and randomly amplified polymorphic DNA analysis of Vibrio vulnificus biotypes. Syst Appl Microbiol 1993; 16: 303–9.CrossRefGoogle Scholar
18.Pedersen, K, Larsen, JL. rRNA gene restriction patterns of Vibrio anguillarum serogroup O1. Dis Aquat Org 1993; 16: 121–6.CrossRefGoogle Scholar
19.Sørensen, UBS, Larsen, JL. Serotyping of Vibrio anguillarum. Appl Environ Microbiol 1986; 51: 593–7.CrossRefGoogle ScholarPubMed
20.Kado, CI, Liu, ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145: 1365–73.CrossRefGoogle ScholarPubMed
21.Macrina, FL, Kopecko, DJ, Jones, KR, Ayers, DJ, McCowen, KR. A multiple plasmid-containing Escherichia coli strain: Convenient source of size reference plasmid molecules. Plasmid 1978; 1: 417–20.CrossRefGoogle ScholarPubMed
22.Threlfall, EJ, Rowe, B, Ferguson, JL, Ward, LR. Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204C isolated in Britain. J Hyg 1986; 97: 419–26.CrossRefGoogle ScholarPubMed
23.Olsen, JE. Improved method for rapid isolation of plasmid DNA from wild-type Gram-negative bacteria for plasmid restriction profile analysis. Lett Appl Microbiol 1990; 10: 209–12.CrossRefGoogle ScholarPubMed
24.Austin, B, Alsina, M, Austin, AD, et al. Identification and typing of Vibrio anguillarum: A comparison of different methods. Syst Appl Microbiol 1995; 18: 285–302.CrossRefGoogle Scholar
25.Grimont, F, Grimont, PAD. Determination of rRNA gene restriction patterns. In: Howard, J, Whitecombe, DM, eds. Methods in molecular biology, vol. 46: Diagnostic bacteriology protocols. Ottawa: Humana Press Inc., 1995: 181–200.CrossRefGoogle Scholar
26.Grimont, F, Chevrier, D, Grimont, PAD, Lefèvre, M, Guesdon, JL. Acethylaminofluorene – labelled ribosomal RNA for use in molecular epidemiology and taxonomy. Res Microbiol 1989; 140: 447–54.CrossRefGoogle Scholar
27.Priest, F, Austin, B. Modern bacterial taxonomy, 2nd ed.. London: Chapman & Hall, 1993.Google Scholar
28.Hunter, PR, Gaston, MA. Numerical index of discriminatory ability of typing system: an application of Simpson's index of diversity. J Clin Microbiol 1988; 26: 2465–6.CrossRefGoogle ScholarPubMed
29.Crosa, JH, Schiewe, MH, Falkow, S. Evidence for plasmid contribution to virulence of the fish pathogen Vibrio anguillarum. Infect Immun 1977; 18: 509–13.CrossRefGoogle ScholarPubMed
30.Crosa, JH. A plasmid associated with virulence in the marine fish pathogen Vibrio anguillarum specifies an iron-sequestering system. Nature 1980; 284: 566–8.CrossRefGoogle ScholarPubMed
31.Crosa, JH, Hodges, LL, Schiewe, MH. Curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen Vibrio anguillarum. Infect Immun 1980; 27: 897–902.CrossRefGoogle ScholarPubMed
32.Actis, LA, Potter, SA, Crosa, JH. Iron-regulated outer membrane protein OM2 of Vibrio anguillarum is encoded by virulence plasmid pJM1. J Bacteriol 1985; 161: 736–42.CrossRefGoogle ScholarPubMed
33.Pedersen, K, Larsen, JL. Evidence for the existence of distinct populations of Vibrio anguillarum serogroup O1 based on plasmid contents and ribotypes. Appl Environ Microbiol 1995; 61: 2292–6.CrossRefGoogle ScholarPubMed
34.Skov, MN, Pedersen, K, Larsen, JL. Comparison of pulsed-field gel electrophoresis, rib typing, and plasmid profiling for typing of V. anguillarum serovar O1. Appl Environ Microbiol 1995; 61: 1540–5.CrossRefGoogle Scholar