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Survey of Vibrio cholerae O1 and its survival over the winter in marine water of Port of Osaka

Published online by Cambridge University Press:  23 September 2003

K. MIYAGI ,
T. NAKANO ,
T. YAGI ,
M. HANAFUSA ,
S. IMURA ,
T. HONDA ,
Y. NAKANO  and
K. SANO
K. MIYAGI
Affiliation:
Department of Microbiology, Osaka Medical College, Takatsuki-shi, Osaka 569-8686, Japan Osaka Quarantine Station, Osaka-shi, Osaka 552-0021, Japan Present address: Kobe Quarantine Station, Kobe, Japan.
T. NAKANO
Affiliation:
Department of Microbiology, Osaka Medical College, Takatsuki-shi, Osaka 569-8686, Japan
T. YAGI
Affiliation:
Osaka Quarantine Station, Osaka-shi, Osaka 552-0021, Japan
M. HANAFUSA
Affiliation:
Osaka Quarantine Station, Osaka-shi, Osaka 552-0021, Japan
S. IMURA
Affiliation:
Kobe Quarantine Station, Kobe-shi, Hyogo 652-0866, Japan
T. HONDA
Affiliation:
Research Institute for Microbial Diseases, Osaka University, Suita-shi, Osaka 565-0871, Japan
Y. NAKANO
Affiliation:
Osaka Quarantine Station, Osaka-shi, Osaka 552-0021, Japan
K. SANO
Affiliation:
Department of Microbiology, Osaka Medical College, Takatsuki-shi, Osaka 569-8686, Japan
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Abstract

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The survey of Vibrio cholerae O1 in marine area was carried out in the Port of Osaka, Japan in 1987–2001, and 51 V. cholerae O1 strains were isolated. All strains were identified to be of El Tor biotype, Ogawa serotype and classic Ubon Kappa-phage type, and were cholera toxin (CT)-negative and CT gene-negative. In order to clarify certain ecological aspects of V. cholerae O1 in the marine environment of the temperate zone, we performed molecular analysis of the isolated strains using pulsed-field gel electrophoresis (PFGE) with NotI and SfiI restriction enzymes. We found the indistinguishable strains by DNA analysis using PFGE with strains passed for 1 year, and also found the closely related strains with that passed for 3 and 12 years. Those results indicated that V. cholerae O1 can survive over one winter at least, and that it survives in marine water for a long time by undergoing continuous mutation.

Type
Research Article
Information
Epidemiology & Infection , Volume 131 , Issue 1 , August 2003 , pp. 613 - 619
Copyright
© 2003 Cambridge University Press