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Survival of virulent Legionella pneumophila in aerosols

Published online by Cambridge University Press:  15 May 2009

P. Hambleton ,
M. G. Broster ,
P. J. Dennis ,
R. Henstridge ,
R. Fitzgeorge  and
J. W. Conlan
P. Hambleton
Affiliation:
Public Health Laboratory Service, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire
M. G. Broster
Affiliation:
Chemical Defence Establishment, Porton Down, Salisbury, Wiltshire
P. J. Dennis
Affiliation:
Public Health Laboratory Service, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire
R. Henstridge
Affiliation:
Chemical Defence Establishment, Porton Down, Salisbury, Wiltshire
R. Fitzgeorge
Affiliation:
Public Health Laboratory Service, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire
J. W. Conlan
Affiliation:
Public Health Laboratory Service, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire
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Summary

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Aqueous suspensions of virulent Legionellapneumophila grown on solid medium retained virulence and aerosol survival characteristics for several months. Significant numbers of viable organisms were recovered from aerosols held at various relative humidities (r.h.) for up to 2 h. The organisms survived best at 65% r.h. and were least stable at 55% r.h.

Exponential phase broth-grown organisms survived poorly in aerosols in comparison with stationary phase broth cultures or organisms grown on solid medium, suggesting that the metabolic status of Legionella pneumophila organisms may be an important factor affecting their ability to survive in aerosols and cause respiratory disease.

Type
Research Article
Information
Journal of Hygiene , Volume 90 , Issue 3 , June 1983 , pp. 451 - 460
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Anderson, J. D. (1966). Biochemical studies of lethal processes in aerosols of Escherichia coli. Journal of General Microbiology 45, 303–313.CrossRefGoogle ScholarPubMed
Anderson, J. D. & Cox, C. S. (1967). Microbial survival. Symposia of the Society for General Microbiology 17, 203–226.Google Scholar
Arnow, P. M., Chou, T., Weil, D., Shapiro, E. N. & Kretzschmar, C. (1982). Nosocomial legionnaires’ disease caused by aerosolized tap water from respiratory devices. Journal of Infectious Diseases 146, 460–467.CrossRefGoogle ScholarPubMed
Band, J. P., La Venture, M. & Davis, J. P. (1981). Epidemic legionnaires’ disease: airborne transmission down a chimney. Journal of the American Medical Association 245, 2404–2407.CrossRefGoogle Scholar
Baskerville, A., Broster, M., Fitzgeorge, R. B., Hambleton, P. & Dennis, P. J. (1981). Experimental transmission of legionnaires’ disease by exposure to aerosols of Legionella pneumophila. Lancet ii, 1389–1390.CrossRefGoogle Scholar
Baskerville, A., Dowsett, A. B., Fitzgeoroe, R. B., Hambleton, P. & Broster, M. (1982). Ultrastructural changes in the lungs in experimental legionnaires’ disease. Journal of Pathology (In the Press).Google Scholar
Bateman, J. B., McCaffrey, P. A., O'connor, R. J. & Monk, G. W. (1961). Relative humidity and the killing of bacteria. The survival of damp Serratia marcescens in air. Applied Microbiology 9, 576–581.CrossRefGoogle ScholarPubMed
Benbough, J. E. (1967). Death mechanisms in airborne Escherichia coli. Journal of General Microbiology 47, 325–333.CrossRefGoogle ScholarPubMed
Benbough, J. E. (1969). Factors affecting the toxicity of oxygen towards airborne coliform bacteria. Journal of General Microbiology 56, 241–250.CrossRefGoogle ScholarPubMed
Benbough, J. E., Hambleton, P., Martin, K. L. & Strange, R. E. (1972). Effect of aerosolization on the transport of α-methyl glucoside and galactosides into Escherichia coli. Journal of General Microbiology 72, 511–520.CrossRefGoogle Scholar
Berendt, R. F. (1980). Survival of Legionella pneumophila in aerosols: effect of relative humidity. Journal of Infectious Diseases 141, 689.CrossRefGoogle ScholarPubMed
Berendt, R. F. (1981). Influence of blue-green algae (cyanobacteria) on survival of Legionella pneumophila in aerosols. Infection and Immunity 32, 690–692.CrossRefGoogle ScholarPubMed
Berendt, R. F., Young, H. W., Allen, R. G. & Knutsen, G. L. (1980). Dose response of guinea pigs experimentally infected with aerosols of Legionella pneumophila. Journal of Infectious Diseases 141, 186–192.CrossRefGoogle ScholarPubMed
Broome, C. V., Goings, S. A. J., Thacker, S. B., Vogt, R. L., Beaty, H. N., Faser, D. W. & Field Investigation Team. (1979). The Vermont epidemic of legionnaires’ disease. Annals of Internal Medicine 90, 573–577.CrossRefGoogle ScholarPubMed
Cordes, L. G. & Fraser, D. W. (1980). Legionellosis. Legionnaires’ disease; Pontiac fever. Medical Clinics of North America 64, 395–416.CrossRefGoogle ScholarPubMed
Cordes, L. G., Fraser, D. W., Skaliy, P., Perlino, C. A., Elsea, W. R., Mallison, G. F. & Hayes, P. S. (1980). Legionnaires’ disease outbreak at an Atlanta country club. Evidence for spread from an evaporative condenser. American Journal of Epidemiology 11, 425–431.CrossRefGoogle Scholar
Cox, C. S. (1966). The survival of Escherichia coli atomized into air and into nitrogen from distilled water and from solutions of protecting agents as a function of relative humidity. Journal of General Microbiology 43, 383–399.CrossRefGoogle Scholar
Cox, C. S. (1969). The cause of loss of viability of airborne Escherichia coli K 12. Journal of General Microbiology 57, 77–80.CrossRefGoogle Scholar
Cox, C. S. (1971). Aerosol survival of Pasteurella tularensis disseminated from the wet and dry states. Applied Microbiology 21, 482–486.CrossRefGoogle ScholarPubMed
Dennis, P. J., Taylor, J. A. & Barrow, G. I. (1981). Phosphate buffered low sodium chloride blood agar medium for Legionella pneumophila. Lancet ii, 636.CrossRefGoogle Scholar
Dewald, R. R., Browall, K. W., Schaefer, L. D. & Messer, A.,(1967). Effect of water vapour on lyophilized Serratia marcescens and Escherichia coli. Applied Microbiology 15, 1299–1302.CrossRefGoogle Scholar
Dondero, T. J. Jr, Rendtorff, R. C., Mallison, G. F., Weeks, R. M., Levy, J., Wong, E. W. & Scjiaffner, W.,(1980). Outbreak of legionnaires’ disease associated with a contaminated air conditioning cooling tower. New England Journal of Medicine 302, 365–370.CrossRefGoogle ScholarPubMed
Druett, H. A. (1969). A mobile form of the Henderson apparatus. Journal of Hygiene 67, 437–448.CrossRefGoogle ScholarPubMed
Edelstein, P. H. (1981). Improved semi-selective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. Journal of Clinical Microbiology 14, 298–303.CrossRefGoogle Scholar
Evans, C. G. T. & Harris-Smith, R.,(1971). A novel selective method for the production of Bacillus subtilis var. niger spores. Journal of Applied Bacteriology 34, 215–219.CrossRefGoogle ScholarPubMed
Feeley, J. C., Gibson, R. J., Gorman, G. W., Langford, N. C., Rasheed, J. K., Mackel, D. C. & Baine, W. B. (1979). Charcoal yeast extract agar: primary isolation medium for Legionella pneumophila. Journal of Clinical Microbiology 10, 437–441.CrossRefGoogle ScholarPubMed
Fisher-Hoch, S. P., Bartlett, C. L. H., Tobin, J. O.'H., Gillett, M. B., Nelson, A. M., Pritchard, J. E., Smith, M. G., Swann, R. A., Talbot, J. M. & Thomas, J. A. (1981). Investigation and control of an outbreak df Legionnaires’ disease in a district general hospital. Lancet i, 932–936.CrossRefGoogle Scholar
Fliermans, C. B., Cherry, W. B., Orrison, L. H., Smith, S. J., Tison, D. L. & Pope, D. H. (1981). Ecological distribution of Legionella pneumophila. Applied and Environmental Microbiology 41, 9–16.CrossRefGoogle ScholarPubMed
Fraser, D. W. (1980). Legionellosis: Evidence of airborne transmission. Annals of the New York Acadamy of Sciences 353, 61–66.CrossRefGoogle ScholarPubMed
Glick, T. H., Gregg, M. B., Berman, B., Mallison, G. F., Rhodes, W. W. Jr. & Kassanoff, I.,(1978). Pontiac fever: an epidemic of unknown etiology in a health department. I. Clinical and epidemiologic aspects. American Journal of Epidemiology 107, 149–160.CrossRefGoogle Scholar
Goldberg, L. J., Watkins, H. M. S., Boerke, E. E. & Chatigny, M. A. (1958). The use of a rotating drum for the study of aerosols over extended periods of time. American Journal of Hygiene 68, 85–93.Google Scholar
Hambleton, P., Strange, R. E. & Benbough, J. B. (1972). The relationship between bacterial metabolic activity and survival in aerosols. Microbiological Research Establishment Report no. 62.Google Scholar
Harper, G. J., Hood, A. M. & Morton, J. D. (1958). Airborne microorganisms: A technique for studying their survival. Journal of Hygiene 56, 364–370.CrossRefGoogle ScholarPubMed
Hess, G. E. (1965). Effects of oxygen on aerosolized Serratia marcescens. Applied Microbiology 13, 781–787.CrossRefGoogle ScholarPubMed
Katz, S. M., Habib, W. A., Hammel, J. M. & Nash, P.,(1982). Lack of airborne spread of infection by Legionella pneumophila among guinea pigs. Infection and Immunity 38, 620–622.CrossRefGoogle Scholar
Mallison, G. F. (1980). Legionellosis: environmental aspects. Journal of the American Medical Associaiion 245, 67–70.Google Scholar
May, K. R. & Harper, G. J. (1957). The efficiency of various liquid impinger samplers in bacterial aerosols. British Journal of Industrial Medicine 14, 287–297.Google ScholarPubMed
McDade, J. E., Shepard, C. C., Fraser, D. W., Tsai, R. F., Redus, M. A. & Dowdle, W. R. & Laboratory Investigation Team. (1977). Legionnaires' Disease: Isolation of a bacterium and demonstration of its role in other respiratory disease. New England Journal of Medicine 297, 1197–1203.CrossRefGoogle ScholarPubMed
Morris, G. K., Patton, C. M., Feeley, J. C., Johnson, S. E., Gorman, G., Martin, W. T., Skaliy, P., Mallison, G. F., Politi, B. I. & Mackel, M. S. (1979). Isolation of the legionnaires' bacterium from environmental samples. Annals of Internal Medicine 90, 664–666.CrossRefGoogle ScholarPubMed
Page, F. C. (1967). Taxonomic criteria for limax amoebae with descriptions of three new species of Hartmanella and three of Vohlkampfia. Journal of Protozoology 14, 499–521.CrossRefGoogle Scholar
Politi, B. D., Fraser, D. W., Mallison, G. F., Mohatt, J. V., Morris, G. K., Patton, C. M., Feeley, J. C., Telle, R. D. & Bennett, J. V. (1979). A major focus of legionnaires' disease in Bloomington, Indiana. Annals of lnternal Medicine 90, 587–591.CrossRefGoogle Scholar
Ristroph, J. D., Hedlund, K. W. & Allen, R. G. (1980). Liquid medium for growth of Legionella pneumophila. Journal of Clinical Microbiology 11, 19–21.CrossRefGoogle ScholarPubMed
Strange, R. E. & Cox, C. S. (1976). Survival of dried and airborne bacteria. Symposia of the Society for General Microbiology 26, 111–154.Google Scholar
Thacker, S. B., Bennett, J. V., Tsai, T. F., Fraser, D. W., McDade, J. E., Shepard, C. C., Williams, K. H. Jr., Stuart, W. H., Dull, H. B. & Eickhoff, T. C. (1978). An outbreak in 1965 of severe respiratory illness caused by the legionnaires' disease bacterium. Journal of Infectious Diseases 138, 512–519.CrossRefGoogle ScholarPubMed
Tobin, J. O'H., Beare, J. & Dunhill, M. S. (1980). Legionnaires' disease in a transplant unit: isolation of causative agent from shower baths. Lancet ii, 118–121.CrossRefGoogle Scholar
Tobin, J. O'H., Swann, R. A. & Bartlett, C. J. R. (1981). Isolation of Legionella pneumophila from water systems: methods and preliminary results. British Medical Journal 282, 515–517.CrossRefGoogle ScholarPubMed
Webb, S. J. (1967). The influence of oxygen and inositol on the survival of semi-dried microorganisms. Canadian Journal of Microbiology 13, 733–742.CrossRefGoogle Scholar