Hostname: page-component-848d4c4894-wg55d Total loading time: 0 Render date: 2024-06-08T15:19:44.500Z Has data issue: false hasContentIssue false

The titration of vaccinial neutralizing antibody on chorio-allantoic membranes

Published online by Cambridge University Press:  15 May 2009

E. A. Boulter
Affiliation:
Microbiological Research Establishment, Porton, near Salisbury, Wiltshire
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A study has been made of various factors involved in performing neutralization tests with vaccinia virus on chorio-allantoic membranes.

The common practice of using a single serum concentration has been shown to result in a test which is qualitative only. The linear relationship between the logarithm of the pock count of the surviving virus and the logarithm of the serum dilution can be used to determine the dilution of serum which will neutralize 50% of a virus suspension. By this method it is possible to distinguish between sera whose potencies vary by about fourfold.

I gratefully acknowledge the advice and criticism given by Dr J. C. N. Westwood and Dr I. A. Macpherson, as well as the help given by Mr S. Peto with the statistical treatment of results. My thanks are also due to Mr H. B. Maber and Corporal M. Fitzpatrick, R.A.M.C., for their technical assistance.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1957

References

REFERENCES

Blattner, R. J., Heys, F. M. & Gollub, S. W. (1943). Antibody-response to cutaneous inoculation with vaccinial virus in human subjects, utilising the egg-protection technic. I. Serum-virus neutralisation. J. Immunol. 46, 207.CrossRefGoogle Scholar
Briody, B. A., Ledinko, N. & Stannard, C. (1951). Studies on vaccinia virus. II. Neutralisation of vaccinia virus by normal guinea pig serum. J. Immunol. 67, 413.CrossRefGoogle ScholarPubMed
Burnet, F. M. & Lush, D. (1939). Herpes simplex. Studies on the antibody content of human sera. Lancet, 1, 629.CrossRefGoogle Scholar
Burnet, F. M., Keogh, E. V. & Lush, D. (1937). The immunological reactions of the filterable viruses. Aust. J. exp. Biol. med. Sci. 15, 231.CrossRefGoogle Scholar
Downie, A. W. & McCarthy, K. (1950). The viruses of variola, vaccinia, cowpox, and ectromelia. Neutralisation tests on the chorio-allantois with unabsorbed and absorbed sera. Brit. J. exp. Path. 31, 789.Google Scholar
Dulbecco, R., Vogt, M. & Strickland, A. G. R. (1956). A study of the basic aspects of neutralisation of two animal viruses, western equine encephalitis and poliomyelitis. Virology, 2, 162.CrossRefGoogle ScholarPubMed
Horsfall, F. L. Jr (1939). Neutralization of epidemic influenza virus. J. exp. Med. 70, 209.CrossRefGoogle ScholarPubMed
Jawetz, E. & Coleman, V. R. (1952). Studies on herpes simplex virus. III. The neutralisation of egg-adapted herpes virus by human sera in ovo. J. Immunol. 68, 645.CrossRefGoogle ScholarPubMed
Keogh, E. V. (1936). Titration of vaccinia virus on the chorio-allantoic membrane of the chick embryo and its application to immunological studies of neuro-vaccinia. J. Path. Bact. 43, 441.CrossRefGoogle Scholar
Morgan, I. M. (1945). Quantitative study of the neutralisation of western equine encephalomyelitis virus by its antiserum and the effect of complement. J. Immunol. 50, 359.CrossRefGoogle Scholar
Parker, R. F. (1939). The neutralisation of vaccinia virus by serum of vaccinia-immune animals. J. Immunol. 36, 147.CrossRefGoogle Scholar
Rose, H. M. & Molloy, E. (1947). Cutaneous reactions with the virus of herpes simplex. J. Immunol. 56, 287.CrossRefGoogle ScholarPubMed
Sternberg, G. M. (1892). Practical results of bacteriological researches. Trans. Ass. Amer. Phycns, 7, 68.Google Scholar
Westwood, J. C. N., Phipps, P. H. & Boulter, E. A. (1957). The titration of vaccinia virus on the chorio-allantoic membrane of the developing chick embryo. J. Hyg., Camb., 55, 123.CrossRefGoogle Scholar