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The use of sorbitol-MacConkey agar in conjunction with a specific antiserum for the detection of Vero cytotoxin-producing strains of Escherichia coli O 157

Published online by Cambridge University Press:  15 May 2009

H. Kleanthous ,
N. K. Fry ,
H. R. Smith ,
R. J. Gross  and
B. Rowe
H. Kleanthous
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
N. K. Fry
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
H. R. Smith
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
R. J. Gross
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
B. Rowe
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
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Using DNA probes specific for the genes encoding Vero cytotoxins 1 and 2 in hybridization experiments on faecal samples. Vero cytotoxin-producing Escherichia coli (VTEC) of serogroup 0 157 were detected in 21 of 63 cases of haemorrhagic colitis, 9 of 31 cases of non-bloody diarrhoea and 14 of 68 cases of haemolytic uraemic syndrome. Compared with these results sorbitol-MacConkey agar in conjunction with a specific 0 157 antiserum gave a sensitivity of 62% in haemorrhagic colitis, 56% in non-bloody diarrhoea and 57% in haemolytic uraemic syndrome.The specificity of this method was 100% in all three groups. This demonstrates that sorbitol-MacConkey agar is a useful screening method for the detection of VTEC of serogroup O 157 when used in conjunction with a specific homologous antiserum. However, this method does not detect VTEC belonging to other serogroups and such strains were found, particularly in cases of haemolytic uraemic syndrome.

Type
Research Article
Information
Epidemiology & Infection , Volume 101 , Issue 2 , October 1988 , pp. 327 - 335
Copyright
Copyright © Cambridge University Press 1988

References

Borczyk, A. A., Karmali, M. A., Lior, H. & Duncan, L. M. C. (1987). Bovine reservoir for Verotoxin–producing Escherichia coli 0 157:H7. Lancet i, 98.CrossRefGoogle Scholar
Bopp, C. A., Greene, K. D., Downes, E. P., Sowers, E. G., Wells, J. G. & Wachsmuth, I. K. (1987). Unusual Verotoxin–producing Escherichia coli associated with haemorrhagic colitis. Journal of Clinical Microbiology 25, 1486–1489.CrossRefGoogle Scholar
Cowan, S. T. (1974). Cowan and Steel's Manual for the identification of Medical Bacteria. 2nd ed.Cambridge: Cambridge University Press.Google Scholar
Farmer, J. J. & Davis, B. R. (1985). H7 antiserum-sorbitol fermentation medium for detecting Escherichia coli O 157: H7 associated with haemorrhagic colitis. Journal of Clinical Microbiology. 22, 620–625.Google Scholar
Fletcher, R. H., Fletcher, S. W. & Wagner, E. H. (1982). Clinical Epidemiology- The Essentials, p. 46. Baltimore: The Williams and Wilkins. Co.Google Scholar
Gransden, W. R., Damm, M. A. S., Anderson, J.D., Carter, J. E. & Lior, H. (1986). Further evidence associating haemolytic uraemic syndrome with infection by Verotoxin–producing Escherichia coli O 157: H7 Journal of Infectious Diseases 154, 522–524.Google Scholar
Gross, R. J. & Rowe, B. (1985). Serotyping of Escherichia coli. In The Virulence of Escherichia coli. Reviews and Methods (ed. Sussman, M.), p. 345–363. London: Academic Press.Google Scholar
Haldane, D. J. M., Damm, M. A. S. & Anderson, J. D. (1986). Improved biochemical screening procedure for small clinical laboratories for Vero (Shiga–like) – toxin–producing strains of Escherichia coli O 157. H7. Journal of Clinical Microbiology 24, 652–653.CrossRefGoogle Scholar
Harris, A. A., Kaplan, R. L. & Goodman, L. J. (1985). Results of a screening method in a 12-month stool survey for Escherichia coli O 157. H7. Journal of Infectious Diseases 152, 775–778.Google Scholar
Karmali, M. A., Steel, B. T., Petric, M. & Lim, C.(1983). Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin–producing Escherichia coli in stools. Lancet i, 619–620.CrossRefGoogle Scholar
Karmali, M. A., Petric, M., Lim, C., Fleming, P. C., Arbus, G. S. & Lior, H. (1985). The association between idiopathic hemolytic uremic syndrome and infection by Verotoxin–producing Escherichia coli. Journal of Infectious Diseases 151, 775–782.Google Scholar
Karmali, M. A., Petric, M., Louie, S. & Cheung, R. (1986). Antigenic heterogeneity of Escherichia coli Verotoxins. Lancet i, 164–165.CrossRefGoogle Scholar
Konowalchuk, J., Speirs, J. I. & Stavric, S. (1977). Vero response to a cytotoxin of Escherichia coli. Infection and Immunity 18, 775–779.Google Scholar
Lior, H. & Borczyk, A. A. (1987). False positive identifications of Escherichia coli O 157. Lancet i, 333.Google Scholar
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.Google Scholar
March, S. B. & Ratnam, S. (1986). Sorbitol–MacConkey medium for detection of Escherichia coli associated with hemorrhagic colitis. Journal of Clinical Microbiology 23, 869–972.CrossRefGoogle ScholarPubMed
Morgan, G. M., Newman, C., Palmer, S. R., Allen, J. B., Shepherd, W., Rampling, A. M., Warren, R. E., Gross, R. J., Scotland, S. M. & Smith, H. R. (1988). A community outbreak of haemorrhagic colitis due to Verotoxin producing Escherichia coli O 157: H7. Epidemiology and Infection. In Press.Google Scholar
Newland, J. W., Strockbine, N. A., Miller, S. F., O'Brien, A. D. & Holmes, R. K. (1985). Cloning of Shiga–like structural genes from a toxin converting phage of Escherichia coli. Science 230, 179–181.CrossRefGoogle ScholarPubMed
Notenboom, R. H., Borczyk, A., Karmali, M. A. & Duncan, L. M. C. (1987). Clinical relevance of a serological cross–reaction between Escherichia coli O 157 and Brucella abortus. Lancet i, 745.CrossRefGoogle Scholar
O'brien, A. D. & Holmes, R. (1987). Shiga and Shiga–like toxins. Microbiological Reviews. pp. 206–220.Google Scholar
Riley, L. W., Remis, R. S., Helgerson, S. D., Mcgee, H. B., Wells, J. G., Davis, B. R., Hebert, R. J., Olcott, E. S., Johnson, L. M., Hargrett, N. T., Blake, P. A. & Cohen, M. L. (1983). Hemorrhagic colitis associated with a rare Escherichia coli serotype. New England Journal of Medicine 308, 681–685.Google Scholar
Scotland, S. M., Day, N. P. & Rowe, B. (1980). Production of a cytotoxin affecting Vero cells by strains of Escherichia coli belonging to traditional enteropathogenic serogroups. FEMS Microbiology Letters. 7, 15–17.Google Scholar
Scotland, S. M., Smith, H. R., Willshaw, G. A. & Rowe, B. (1983). Vero cytotoxin production in strains of Escherichia coli is determined by genes carried on bacteriophage. Lancet ii, 216.CrossRefGoogle Scholar
Scotland, S. M., Smith, H. R. & Rowe, B. (1985). Two distinct toxins active on Vero cells from Escherichia coli O 157. Lancet ii, 885–886.Google Scholar
Scotland, S. M., Rowe, B., Smith, H. R., Willshaw, G. A. & Growss, R. J. (1988). Vero cytotoxin–producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA Probes. Journal of Medical Microbiology 25. 237–243.CrossRefGoogle ScholarPubMed
Smith., H. R., Rowe, B., Gross, R. J., Fry, N. K. & Scotland, S. M. (1987). Haemorrhagic colitis and Vero cytotoxin–producing Escherickia coli in England and Wales. Lancet i, 1062–1065.Google Scholar
Smith, H.Williams, Green P. & Parsell, Z. (1983). Vero cell toxins in Escherichia coli and related bacteria; transfer by phage and conjugation and toxic action in laboratory animals, chickens and pigs. Journal of General Microbiology 129, 3121–3137.Google Scholar
Taylor, C. M., White, R. H. R., Winterborn, M. H. & Rowe, B. (1986). Haemolytic–uraemic syndrome: clinical experience of an outbreak in the West Midlands. British Medical Journal 292, 1513–1516.CrossRefGoogle ScholarPubMed
Von Gassek, C., Gautier, E., Steck, A., Siebenmann, R. E. & Oechslin, R. (1955). Hämolytisch–urämische syndrome: bilaterale nierenrindennekrusen bein akuten erworbenen hämolytischen anämien. Schweizerische Medizinische Wochenschrift. 85, 905–909.Google Scholar
Walker, C. W., Upson, R. & Warren, R. E. (1988). Haemorrhagic colitis: detection of Verotoxin producing Escherichia coli O 157 in a clinical microbiology laboratory. Journal of Clinical Pathology 41, 80–84.CrossRefGoogle Scholar
Wells, J. G., Davis, B. R., Wachsmuth, I. K., Riley, L. W., Remis, R. S., Sokolow, R. & Morris, G. K. (1983). Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. Journal of Clinical Microbiology 18, 512–520.CrossRefGoogle ScholarPubMed
Willshaw, G. A., Smith, H. R., Scotland, S. M. & Rowe, B. (1985). Cloning of genes determining the production of Vero cytotoxin by Escherichia coli. Journal of General Microbiology 131, 3047–3053.Google ScholarPubMed
Willshaw, G. A., Smith, H. R., Scotland, S. M., Field, A. M. & Rowe, B. (1987). Heterogeneity of Escherichia coli phages encoding Vero cytotoxins: comparison of cloned sequences determining VT1 and VT2 and development of specific gene probes. Journal of General Microbiology 133, 1309–1317.Google Scholar