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Cognitive Impairment and the correlation with genetic Expression of GAD67, Gad65 and GABA beta2 Using Human Induced Pluripotent Stem Cells

Published online by Cambridge University Press:  01 September 2022

D. Khalifa ,
H. Gabr ,
H. Fathy ,
H. Abdou  and
M. Batrawy
D. Khalifa*
Affiliation:
Kasr Al Ainy hospitals, Cairo University, Psychiatry, Cairo, Egypt
H. Gabr
Affiliation:
Kasr Al Ainy hospitals, Cairo University, Clinical Pathology, Cairo, Egypt
H. Fathy
Affiliation:
Kasr Al Ainy hospitals, Cairo University, Psychiatry, Cairo, Egypt
H. Abdou
Affiliation:
Kasr Al Ainy hospitals, Cairo University, Psychiatry, Cairo, Egypt
M. Batrawy
Affiliation:
Kasr Al Ainy hospitals, Cairo University, Psychiatry, Cairo, Egypt
*
*Corresponding author.

Abstract

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Introduction

Alteration of GABergic neurotransmission is accused to be sharing in the cognitive impairment in schizophrenia. Exploring the relation between the neuronal expression of GABergic genes and cognitive impairment in living patients through modeling of schizophrenia is an important step to know more about the core of the pathophysiology of this disorder

Objectives

Altered genetic expression of GAD 67 may have an important role in the pathophysiology of cognitive impairment in schizophrenia

Methods

. Reprogramming of human fibroblasts into human induced pluripotent stem cells (hIPSc) then neuronal differentiation was performed in 20 patients presenting with schizophrenia and 20 matched controls. Real time Polymerase chain reaction was done for measurement of genetic expression of GAD 65, GAD 67 and GABA beta 2. The Digit Symbol task, block design, block design task and similarities tasks from the Wechsler Adult Intelligence Scale., Trail A and Trail B making tests in addition to Rey-Osterrieth Complex Figure Test (ROCF) were applied to measure cognitive functions .

Results

There were lower means of GAD65, GAD67 and GABA beta2genetic expression in the patients group with significant statistical difference between the 2 groups. The down regulation of GAD 67 in patients presenting with schizophrenia is positively correlated with impairment in executive functions.

Conclusions

GAD 67 gene expression had the most significant correlations with the cognitive assessment in both patients and controls. The presence of those statistically significant correlations in both groups points to the possible role of GAD 67 gene functioning in the pathophysiology of cognitive impairment in schizophrenia

Disclosure

No significant relationships.

Keywords

GAD67Genetic expressionschizophrénia
Type
Abstract
Information
European Psychiatry , Volume 65 , Special Issue S1: Abstracts of the 30th European Congress of Psychiatry , June 2022 , pp. S314
Creative Commons
Creative Common License - CCCreative Common License - BY
This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright
© The Author(s), 2022. Published by Cambridge University Press on behalf of the European Psychiatric Association
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