Ecoregional research has the potential to help address some of the huge challenges facing agriculture in developing countries by developing technologies that work under different agro-ecological conditions, and the processes by which these technologies can be adapted to work in other areas with similar conditions. The CGIAR system has been developing ecoregional research as a new paradigm for over a decade. In this paper we evaluate one of the most ambitious of these initiatives called the Benchmark Area Approach (BAA) pioneered by the International Institute of Tropical Agriculture. We evaluate the BAA against nine good practice criteria for ecoregional research and finding that the approach is delivering, or has the potential to deliver, on all nine. Many of the lessons learnt from this evaluation will be relevant to current and future attempts to undertake co-ordinated multi-locational research for development.

The West and Central Africa Collaborative Maize Research Network (WECAMAN) was established in 1987 to strengthen the capacity and capability of national programmes of West and Central Africa (WCA) to tackle regional constraints to maize production. The Network created several mechanisms for implementing, monitoring and evaluating maize research and development activities, including research project development and implementation, attendance and quality of paper presentation at technical conferences organized by the Network, scientific monitoring tours, consultation visits, mid-term reviews, and end-of-project reviews and impact assessment. WECAMAN's approach to the system of allocating research responsibilities and competitive grants resulted in increased research efficiency and the generation of sustainable technologies that have catalysed increased maize production in the region.

Long-term indicators of soil fertility were assessed by measuring grain yield, soil organic carbon (SOC) and soil Olsen phosphorous for a P-deficient soil. In one set of treatments, goat manure was applied annually for 13 years at 0, 5 and 10 t ha−1, and intercrops of sorghum/cowpea, millet/green gram and maize/pigeonpea were grown. Yield depended on rainfall and trends with time were not identifiable. Manure caused an upward trend in SOC, but 10 t ha−1 manure did not give significantly more SOC than 5 t ha−1. Only 10 t ha−1 manure increased Olsen P. Measurements of both SOC and Olsen P are recommended. In another set of treatments, manure was applied for four years; the residual effect lasted another seven to eight years when assessed by yield, SOC and Olsen P. Treatment with mineral fertilizers provided the same rates of N and P as 5 t ha−1 manure and yields from manure and fertilizer were similar. Fertilizer increased Olsen P but not SOC. Management systems with occasional manure application and intermediate fertilizer applications should be assessed. Inputs and offtakes of C, N and P were measured for three years. Approximately 16, 25 and 11% of C, N and P respectively were stabilized into soil organic matter from 5 t ha−1 a−1 manure. The majority of organic P was fixed as soil inorganic P.

Judicious management of our natural resources is a major pillar of sustainable agriculture, broadly defined. It is, therefore, unfortunate that, until now, research in natural resources management (NRM) has not received sufficient support, or the recognition that other agricultural research topics have had. The Consultative Group on International Agricultural Research (CGIAR) recognized, early on, the complementarities and synergies between NRM research and other themes, notably genetic improvement, and its research Centres have pursued some vigorous programmes for many years. The Technical Advisory Committee (TAC) to the CGIAR was supportive of such programmes from the start and provided advice to the CGIAR System on NRM research strategies. Highlights of such advice over the last 20 years are discussed, including the ecoregional approach, the 1996 study of Priorities and Strategies for Soil and Water Research, and the CGIAR Vision and Strategy of 2000. Recent developments at the Centres leading to the promotion of integrated approaches to NRM (INRM) are described, and a set of priority areas in INRM research for future CGIAR activities is presented.

In this paper we attempt to remove the dichotomy created by distinguishing between participatory and non-participatory breeding programmes by using the degree of client orientation as the basis for an analysis. Although all breeding programmes are implicitly client-oriented, we examine how participatory approaches explicitly increase the extent of client orientation. We briefly review the history of participatory plant breeding (PPB) and analyse the participatory techniques used at different stages of the breeding programme. In common with several other authors, we find that farmer involvement in selecting in the segregating generations may not be an essential component of PPB. However, in some circumstances such collaboration is required and is the subject of a second paper in this series. The purpose of all the techniques used in PPB programmes is to better meet the needs of clients. Thus, breeding programmes can be differentiated by their extent of client-orientation removing the dichotomy involved with the term participatory. We discuss four techniques in the suite of techniques that have been employed by PPB: identifying the target market or clients; using germplasm that can best meet the needs of target clients; matching the environments of the target clients; and product testing in the target market with target clients. Most attention is paid to the last of these four that is often referred to as participatory varietal selection (PVS) and how it is done varies with circumstances. Rice varieties from a client-oriented breeding programme in Nepal were tested in mother and baby trials in Bangladesh. The rapid acceptance of these varieties by farmers illustrates the power of the participatory trials system and the success of a highly client-oriented breeding approach.

Shortened fallow periods lead to a decline in crop yields in traditional mixed-food crop fields in southern Cameroon. Farmers use no inputs such as fertilizers for crop production in these field types. Planted fallows with adapted tree species might sustain or increase crop production under those conditions, as found in other parts of Africa. Two-year-old calliandra tree fallows, with trees planted in alleys, clusters, equidistantly or around plot borders, were compared with two- and four to five-year-old natural fallows for their effect on groundnut, maize and cassava yields on eighteen farmer fields in southern Cameroon. Trial fields covered a wide range of soil and environmental conditions. The tree fallows had no significant effects on the yields of maize and cassava with the exception of the border planting, in which trees were not rigorously pruned back prior to cropping. Here, cassava tuber yields were reduced. Cassava tuber yields declined generally with decreasing planting distance to trees. The yield of groundnut, the most important crop in this field type, was adversely affected. However, maize grain yields were positively related to biomass produced by calliandra trees, notably on soils with pH over about 5.3. The data indicated that yields of all crops could be increased with higher plant densities, irrespective of fallow type. The tree fallows, with the exception of border planting, showed less adaptability than four to five-year-old natural fallows to sites with low crop yields, whereas no difference compared with two-year-old natural fallows was found.

Assimilate storage in vegetative organs is an essential buffer for the source–sink imbalances that inevitably occur in perennial plants. In contrast to temperate trees, little information is available on such storage in tropical perennials, and almost none for Cocos nucifera. This paper describes the chemical nature, quantity and distribution of carbohydrate reserves in coconut plants grown in an environment favourable to production. The study was carried out on the island of Santo (Republic of Vanuatu, Southern Pacific) on twelve 17-year-old adult plants, representative of a large population, which were felled and characterized for root, trunk and crown dry matter, and contents of soluble sugars and starch. Roots were divided into three diameter classes and distal/proximal portions, the trunk into three axial and three or four radial zones, and the crown into petiole, rachis and leaflets for various leaf ages. The aggregate reserve pool size was compared with estimates of incremental demand for assimilates for growth and fruit production. Plants contained little starch but large quantities of sucrose were found, mainly located in the trunk. Less sucrose was present in roots and little in leaf blades. Large glucose and fructose pools were found in leaves, near the apex of the trunk and in the terminal portions of large roots. Aggregate soluble and non-soluble sugar pools were about equivalent to six months of copra production or 51 days of crop growth. More studies are needed on the dynamics of these sugars to evaluate their physiological role, particularly with regards to stress periods and fluctuating demand for fruit filling.

A maize-breeding programme was targeted at resource-poor farmers of eastern India using a client-oriented approach (often called participatory plant breeding). Farmers tested a range of varieties in a participatory varietal selection programme but none proved to be very popular. Information from these trials was used in a breeding programme to develop a broadly-based population from three yellow- and three white-grained maize varieties that were either identified in the trials or had traits liked by farmers. The population was subjected to several cycles of random mating. In the C3, C4 and C6 cycles, subpopulations were extracted and subjected to mass selection over several generations in environments that represented well the target population of environments of resource-poor farmers' fields in eastern India for traits identified by farmers. To identify better the required traits, in some generations the selection was carried out by farmers on a research farm under fertility levels that approximated farmers' practice. The improvement of the subpopulations resulted in several varieties that performed well in research station and on-farm trials. One of them, BVM 2, was released in Jharkhand state, India. In multilocational research station trials, it yielded more than the control variety BM 1 but silked earlier. In the less favourable environments of on-farm trials, its yield superiority, in percentage terms, was higher. Farmers perceived BVM 2 to have better grain quality and stover yield than the local varieties. BVM 2 was specifically bred to meet the needs of the clients (resource-poor farmers with no access to irrigation) and has earlier maturity combined with higher grain yield. The returns were higher from this highly client-oriented approach, than by classical breeding, mainly because uptake was faster as a result of research and extension being done in tandem.

Tubers of white yam (Dioscorea cayenensis-rotundata) are often cut into segments for planting, so-called setts, whose weight and quality strongly influence yield. The effect of a post-harvest gibberellic acid treatment or manual desprouting of seed tubers on the quality of setts was investigated in a combined storage and field experiment over two years in central Ivory Coast. Although post-harvest losses were slightly reduced after four months of storage by both treatments, this had no effect on tuber yield. However, emergence and yield were significantly influenced by both the sprouting state at planting and the origin of a sett with respect to its position on the mother tuber. Limited resources should therefore be directed to apical setts and sprouted setts which have the highest yield potential. A GA3-derived distortion of the spatial distribution of sprouting loci leads to a more homogenous yield for setts of different origins.

The translocation of C and N in a maize-Striga hermonthica association was investigated at three rates of nitrogen application in a glasshouse experiment. The objectives were to measure the transfer of C and N from maize to S. hermonthica and to determine whether the amount of N in the growing medium affected the proportions of C and N transferred. Young plants of maize were labelled in a 13CO2 atmosphere and leaf tips were immersed in (15NH4)2SO4 solution. The Striga×N interaction was not significant for any of the responses measured. Total dry matter for infected maize was significantly smaller than for uninfected maize from 43 to 99 days after planting, but N application increased total dry matter at all sampling times. Infected maize plants partitioned 39–45% of their total dry matter to the roots compared with 28–31% for uninfected maize. Dry matter of S. hermonthica was not affected by the rate of N applied. S. hermonthica derived 100% of its carbon from maize before emergence, decreasing to 22–59% thereafter; the corresponding values for nitrogen were up to 59% pre-emergence and up to 100% after emergence. The relative proportions of nitrogen depleted from the host (up to 10%) were greater than those of carbon (maximum 1.2%) at all times of sampling after emergence of the parasite. The results show that the parasite was more dependent on the host for nitrogen than for carbon.

Soil deficiencies of zinc (Zn) and boron (B) limit crop production in Nepal. Improving the micronutrient status of plants would increase yield and increase micronutrient content of the seeds, leading to better nutrition of the progeny crop and to improved human micronutrient nutrition. The primary micronutrient problem in grain legumes is B deficiency, while in rice (Oryza sativa), Zn deficiency is more important, and wheat (Triticum aestivum) suffers from both deficiencies. A series of field experiments was carried out over two seasons to compare soil fertilization and micronutrient seed priming as methods of improving Zn and B nutrition of each crop. Micronutrient treatments were evaluated for their effect on grain yield and grain micronutrient content. Soil B fertilization increased B content of the grain of lentil (Lens culinaris), chickpea (Cicer arietinum), and wheat by a factor of two to five, while increasing the yield of chickpea only. Soil fertilization with Zn had no effect on yield of any crop, but resulted in a small increase in Zn in wheat grain. Sowing micronutrient-primed seeds had no effect on yield or micronutrient content of the progeny seeds in most cases. During the first season, the primed chickpea seeds failed to emerge at either site, causing complete yield loss, but this negative effect was not observed in the second season with similar priming treatments at nearby sites, and no effect of priming on yield was observed with any other crop in either season.

To understand the impact of residue and tillage management on crop productivity of rice and barley (including grain yield) and soil fertility in a tropical dryland agro-ecosystem, the following six treatments were established: (a) conventional tillage and residue removed (CT−R); (b) conventional tillage and residue retained (CT+R); (c) minimum tillage and residue removed (MT−R); (d) minimum tillage and residue retained (MT+R); (e) zero tillage and residue removed (ZT−R); and (f) zero tillage and residue retained (ZT+R). Minimum total net productivity (TNP) in both barley (the winter season crop) and rice (the rainy season crop) was recorded in the ZT−R treatment and the maximum in the MT+R treatment. In these crops, 83–88% of TNP was represented by above-ground net productivity (ANP). A reduction in tillage, from conventional to zero, especially when combined with residue retention, reduced the crop TNP/weed TNP ratio, reflecting the abundance of weeds with zero tillage. In both crops, minimum tillage increased grain yields, compared with conventional, but zero tillage resulted in reduced yields. Soil microbial biomass carbon (MBC) and nitrogen (MBN) and N-mineralization rates were higher in rice than in barley, and were maximum in the MT+R treatment. Crop productivity and grain yield in different treatments were positively correlated with MBC, MBN, N-mineralization rate and available-N. Strong negative correlations between crop productivity and grain yield with weed TNP and N-uptake were recorded. This study suggests that retention of a small fraction (one-third) of above-ground biomass from the previous crop and its incorporation in the soil through minimum tillage enhances the crop productivity and grain yield of succeeding crops and promotes soil fertility in a cereal based tropical dryland agro-ecosystem.

Organic coffee is one of several types of speciality coffees selling at a premium over mainstream coffees because of distinct origin and flavour, environment-friendly production or socio-economic concerns for the smallholder coffee growers. The demand for organic coffee in Western Europe, North America and Japan exceeds the present supply, which is still small (<1% of annual world production). More than 85% of organic coffees come from Latin America and practically all is (washed) arabica coffee. The production of certified organic coffee follows the principles of organic farming developed in Europe and the United States out of concern for the perceived negative effects of conventional high-input agriculture on health and environment. It claims superior ecological sustainability in combination with sound economic viability. A rather complex and expensive system of certification has to be passed before such coffees can be sold as truly organic. Growers adhering to the strict rules of organic coffee production may to some extent share the concern of the health- and environment-conscious consumers, but they are motivated primarily by the economic benefits from the premium received for certified organic coffee. Nevertheless, there appears to be considerable injustice between the extreme preconditions demanded for ‘organics’ by the largely urban consumer of the industrialized world and the modest rewards received by the organic coffee growers for their strenuous efforts. From an agronomic point of view, there is also considerable ground for criticism on the principles of organic farming when applied to coffee. For instance, to sustain economically viable yield levels (1 t green coffee ha−1 year−1) large additional amounts of composted organic matter will have to come from external sources to meet nutrient requirements (especially N and K). Most smallholders will be unable to acquire such quantities and have to face declining yields. Organic farming does not necessarily reduce incidence of diseases and pests below economically harmful thresholds, while the humid conditions of heavily shaded coffee may actually stimulate the outbreak of others. These and other aspects peculiar to the preconditions of organic coffee production are addressed in this review. It is concluded that the concept of organic farming in its strict sense, when applied to coffee, is not sustainable and also not serving the interests of the producer and consumer as much as the proponents would like us to believe. On the other hand, agronomically and economically sustainable coffee production is feasible by applying best practices of crop production and post-harvest processing.

Pearl millet topcross hybrids (inbred male-sterile seed parent × open-pollinated variety restorer) based on locally adapted varieties and publicly available seed parents provide an ideal entry point into the commercial hybrid seed business, which can stimulate commercial investment by prospective seed producers. To demonstrate this potential, fifteen topcross hybrids made with the widely adapted variety ICMV 221 were evaluated in Eritrea, Sudan and Kenya for overall field performance, and in India for mechanisms of expression of heterosis and for terminal drought tolerance. Across all evaluation environments, the mean yield heterosis was 8%, with a range of −1% to +19%; six hybrids had a statistically significant, positive across-environment yield heterosis (ranging from +11% to +19%). Significant grain yield heterosis in rainy season environments was a consequence of heterosis in both biomass and harvest index, but not necessarily in any specific yield component. Positive grain yield heterosis under terminal drought stress, a common occurrence in millet-growing environments, was related to positive heterosis for grain size. These results are discussed in terms of their support for topcross hybrids as an entry point for prospective millet seed producers, and a scheme presented for the rapid creation, evaluation and marketing of locally adapted topcross hybrids.

Soil phosphorus fractions, maize and cassava P uptakes in natural regrowth fallow and Pueraria phaseoloides fallow systems, as affected by the duration (1–3 years) of fallow, were assessed in 1998 and 1999 in a long-term fallow management trial, established in 1989 at the International Institute of Tropical Agriculture, Ibadan, Nigeria. After one and three years fallow respectively, the mean P accumulation in fallow vegetation was 4.2 and 9.4 kg ha−1 in 1998, and 8.9 and 12.1 kg ha−1 in 1999 for natural fallow. For Pueraria, the corresponding values were 6.3 and 7.4 kg ha−1 in 1998 and 6.1 and 13.5 kg ha−1 in 1999. The positive effects of fallow lengths on dry matter and P accumulation were not as pronounced under Pueraria. Continuous cropping reduced soil pH and depleted Olsen P more than crop-fallow rotations under both fallow systems. An increase in fallow duration enhanced the availability of resin P, NaHCO3-, and NaOH- extractable P fractions, especially under natural fallow. Continuous cropping in a natural fallow system resulted in lower resin inorganic P (Pi) and NaHCO3-extractable inorganic and organic P (Po) fractions, and higher refractory P fractions (concentrated HCl-extractable Pi and Po and residual P) as compared to crop-fallow rotations. Although continuous cropping in the Pueraria system resulted in higher refractory P fractions than the crop-fallow rotations, the effects of fallow length on P fractions were not consistent or pronounced. Maize and cassava P uptakes increased with fallow duration. Phosphorous in maize grain was positively correlated with fallow vegetation P accumulation. Total P uptake by maize was positively but weakly correlated with NaHCO3-Po, Olsen P and fallow vegetation P accumulation.

Crop evapotranspiration (ETc) was measured as evaporative heat flux from a drip-irrigated coffee (Coffea arabica) plantation with 5-year-old trees using the Bowen ratio-energy balance technique. Crop transpiration (T) was determined with the stem heat balance method. Irrigation requirements were determined by comparing the ETc and T with reference evapotranspiration (ETo) derived from the Penman-Monteith equation and expressed as the ETc/ETo (Kc) and T/ETo (Kcb) ratios. Also, relationships were established between ETc and T and class A pan evaporation (ECA). The influence of inter-row vegetation on ETc was analysed, since the measurements were taken in a period of transition between dry-wet seasons. The average Kc value obtained was 1.00. The strong coupling of coffee plants to atmospheric conditions and high sensitivity of coffee plants to large vapour pressure deficits and air/leaf temperatures caused variations in Kcb in relation to ETo. Kcb ranged from 0.67, when ETo exceeded 4 mm d−1, to 1.27 when ETo was less than 2 mm d−1. When vegetation did not occupy the inter-row ground spaces, T represented about 0.87ETc, but 0.68ETc when ground vegetation filled the inter-row spaces.

The Guinea savannas of Nigeria have a high potential for the production of maize because of favourable environmental conditions. Despite this high potential, the yields obtained in farmers' fields are still very low. This is due to soil degradation and nutrient depletion arising from intensification of land use. Nitrogen (N) is the major nutrient limiting maize production in the Guinea savannas where the use of inorganic fertilizers is low. One strategy for improving the productivity of maize is to select varieties that perform well under suboptimal soil N conditions. This study assessed the performance of diverse maize germplasm under a range of N levels. Growth and grain yields differed significantly between the genotypes at all N levels. These variations were more pronounced at zero and 30 kg N ha−1 than at 90 kg N ha−1. Grain yield under N-deficient conditions was correlated with an increased number of ears per plant, stay-green rating, leaf chlorophyll concentration, leaf area index, reduced anthesis–silking interval and reduced days to silking suggesting that these traits are linked to tolerance to N deficiency. One hybrid (Oba Super 2), the drought-tolerant genotypes and four maize genotypes previously selected for tolerance to N-deficient conditions performed better than the widely grown adapted controls under zero and 30 kg N ha−1. At zero N, the grain yield of maize decreased with advances in cycles of selection for tolerance to suboptimal N conditions suggesting that N deficiency in the selection environment was not sufficient to discriminate between genotypes. The good performance of drought-tolerant varieties under suboptimal N conditions suggests that selection for drought tolerance may confer tolerance to N-deficient conditions.

This paper addresses the preservation and use of minor root crop genetic resources, mostly aroids and yams. Conservation is fraught with difficulty: ex situ collections are expensive to maintain and methods for on-farm conservation have not been studied. Conventional breeding strategies present serious limitations when applied to these species. Furthermore, the evaluation and distribution of improved material are as problematical as its conservation. The similarities shared by these species regarding their domestication, breeding constraints and improvement strategies as well as farmers' needs, are briefly reviewed. Based on these biological constraints, we propose a practical alternative to current conservation and breeding strategies. This approach focuses on the geographical distribution of allelic diversity rather than localized ex situ and/or in situ preservation of genotypes. The practical steps are described and discussed. First, a core sample representing the useful diversity of the species is assembled from accessions selected for their diverse and distant geographic origins, wide genetic distances, quality, agronomic performances and functional sexuality. Second, the geographical distribution of this core sample, in vitro via a transit centre, allows the direct use of selected genotypes by farmers or for breeding purposes. Third, the distribution of genes is realized in the form of clones resulting from segregating progenies and, fourth, farmers select clones with local adaptation.

In eastern Zambia, farmers prefer direct seeded improved fallow species like Tephrosia vogelii to other species because of the reduced labour requirement for establishment. Since relying on two locally available provenances of T. vogelii could become ecologically unsustainable, due to pest and disease outbreaks, a wider range of usable direct seeded Tephrosia provenance material is desirable. A farmer participatory evaluation of Tephrosia candida provenances for their effectiveness as improved fallows and provision of construction materials and fuel wood was conducted. Using an indigenous board game called bao, 68 farmers rated the four provenances and five control species/provenances for these benefits. The T. candida provenances were rated high for soil fertility improvement. First year T. candida post-fallow performance showed that maize yields could be obtained that were comparable with yields following Tephrosia vogelii provenances. According to farmers' ratings, T. candida provenances could provide better fuel wood and light construction materials than T. vogelii, although they were not considered to be as good as Sesbania sesban and Senna siamea. Tephrosia candida provenances have the potential to be tested by many farmers on-farm. Farmers' methods of screening trees can complement scientific predictors, and their early integration into the selection and design of agro-forestry systems can improve relevance and adoption.

Reports of cassava root rot disease from different African countries have increased in recent times. Field studies were conducted from July 1998 to October 1999 to determine a reproducible disease assessment method that would allow the comparison of results from different locations and an evaluation of the relationship between disease severity and root yield. Single point disease assessments at 6, 9, 12 and 15 months after planting (MAP) were compared to multiple points assessment based on the area under a disease progress curve (AUDPC). Single point assessments at 12 and 15 MAP, and the AUDPC identified continuous variation (p≤0.01) among the genotypes. However, a consistent result across trials was obtained only with the assessment based on AUDPC. Root dry yield (DYLD) at 15 MAP showed a strong negative correlation with AUDPC (r=−0.74). Regression analysis also confirmed the negative relationship between yield and root rot severity. The five genotypes compared were separated into resistant (91/02324, 30572 and 92/0427) and susceptible (92/0057 and TME-1) groups. It was concluded that root rot disease may cause significant yield loss; however, the magnitude of the yield loss will depend on the susceptibility of the cassava genotype.