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Haploid induction: androgenesis in Musa balbisiana

Published online by Cambridge University Press:  24 January 2008

Frédéric Bakry ,
Akym Assani  and
Florence Kerbellec
Frédéric Bakry
Affiliation:
CIRAD, UPR Multiplication végétative, avenue Agropolis, TA A-75 / 02, 34398 Montpellier Cedex 5, France
Akym Assani
Affiliation:
University of Guelph, Department of Plant Agriculture, Bovey Building, N1G 2W1, Guelph, Ontario, Canada aassani@uoguelph.ca
Florence Kerbellec
Affiliation:
INRA, URPV, Domaine de Duch, 97170 Petit-Bourg, France florence.harbellec@lentilles.inra.fr
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Abstract

Introduction. This protocol describes the production of haploid and dihaploid plants from in vitro anther cultures of M. balbisiana. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. This part describes the required laboratory materials, medium preparation, anther isolation and culture. Results. White, creamy calli emerge from anthers about (6 to 15) weeks after the beginning of the culture.

Keywords

France (Guadeloupe)Musa balbisianamethodsplant biotechnologyhaploidyFrance (Guadeloupe)Musa balbisianaméthodebiotechnologie végétalehaploïdie
Type
Research Article
Information
Fruits , Volume 63 , Issue 1 , January 2008 , pp. 45 - 49
Copyright
© CIRAD, EDP Sciences, 2008

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References

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