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Construction of isomitochondrial and isonuclear strains for recombinational analysis of mitochondrial loci in S. cerevisiae

Published online by Cambridge University Press:  14 April 2009

A. Kruszewska
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 36 Rakowiecka Street, 02-532 Warsaw, Poland
B. Szcześniak
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 36 Rakowiecka Street, 02-532 Warsaw, Poland

Summary

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In the mitochondrial and nuclear genetic background of the 777-3A strain (op1, ade1, α) a set of strains with different nutritional requirements and mating types, carrying the op1 mutation versus its wild-type OP1 allele was constructed. This makes possible the use of strains with the same genetic background in recombinational analysis of mitochondrial loci.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1980

References

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