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Developmental mutants in a homothallic strain of Physarum polycephalum

Published online by Cambridge University Press:  14 April 2009

A. E. Wheals
A. E. Wheals
Affiliation:
Department of Genetics, University of Leicester, Leicester LE1 7RH, England

Summary

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A derivative line of the homothallic Colonia strain of Physarum polycephalum has been isolated which produces plasmodia with high efficiency within clones of amoebae. Using the synergistic effect of ultraviolet light and caffeine, mutants of this line have been isolated which fail to undergo the developmental transition between haploid amoebae and diploid plasmodia (apt mutants). They are isolated by selecting for amoebae which fail to produce plasmodia within clones. Complementation tests of four mutants have shown that they are mutants of four different loci and they are recessive to wild-type. A further analysis of one mutant reveals that the apt-1 locus is unlinked to three other known markers. Crosses of this mutant with heterothallic strains yield progeny which are homothallic indicating that the lesion is not a revertant from a homothallic to a heterothallic mating-type. The use of this system in isolating developmental mutants is discussed.

Type
Research Article
Information
Genetics Research , Volume 21 , Issue 1 , February 1973 , pp. 79 - 86
Copyright
Copyright © Cambridge University Press 1973

References

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