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Effect of pre-treatment with 5-bromouracil in an ultraviolet sensitive strain of Neurospora crassa

Published online by Cambridge University Press:  14 April 2009

Arnold Nemerofsky
Arnold Nemerofsky
Affiliation:
Department of Biology, State University of New York, New Paltz, New York 12561
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5-Bromouracil was shown to enhance the mutation frequency in an ultraviolet sensitive strain of Neurospora crassa (uvs-2) that was previously irradiated with ultraviolet light. The design of the experiment eliminated light or dark repair as possible hypotheses to account for the observed enhancement. Since a similar effect was noted at the rib-1 locus in N. crassa, which is not a UV-sensitive strain, it appears that the error prone repair mechanism in N. crassa may be related to post-replication repair. This enhancement may be attributed to the interference of 5-bromouracil with post-replication repair. It is also postulated that the maximum increase in the mutation frequency occurs when the greatest number of unrepaired pre-mutational UV lesions are repaired in the presence of 5-bromouracil.

Type
Research Article
Information
Genetics Research , Volume 30 , Issue 3 , December 1977 , pp. 265 - 271
Copyright
Copyright © Cambridge University Press 1977

References

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