Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-23T04:53:51.721Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of pre-treatment with 5-bromouracil in an ultraviolet sensitive strain of Neurospora crassa

Published online by Cambridge University Press:  14 April 2009

Arnold Nemerofsky
Arnold Nemerofsky
Affiliation:
Department of Biology, State University of New York, New Paltz, New York 12561
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

5-Bromouracil was shown to enhance the mutation frequency in an ultraviolet sensitive strain of Neurospora crassa (uvs-2) that was previously irradiated with ultraviolet light. The design of the experiment eliminated light or dark repair as possible hypotheses to account for the observed enhancement. Since a similar effect was noted at the rib-1 locus in N. crassa, which is not a UV-sensitive strain, it appears that the error prone repair mechanism in N. crassa may be related to post-replication repair. This enhancement may be attributed to the interference of 5-bromouracil with post-replication repair. It is also postulated that the maximum increase in the mutation frequency occurs when the greatest number of unrepaired pre-mutational UV lesions are repaired in the presence of 5-bromouracil.

Type
Research Article
Information
Genetics Research , Volume 30 , Issue 3 , December 1977 , pp. 265 - 271
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Aoki, S., Boyce, R. P. & Howard-Flanders, P. (1966). Sensitization of E. coli, to radiation by bromouracil. Nature 209, 686–688.CrossRefGoogle ScholarPubMed
Boyce, R. P. & Howard-Flanders, P. (1964). Release of UV light induced thymine dimers in E. coli. Proceedings of the National Academy of Sciences, U.S.A. 51, 293–300.Google Scholar
Brockman, H. E. & de Serres, F. J. (1963). ‘Sorbose Toxicity’ in Neurospora. American Journal of Botany 50, 709–714.CrossRefGoogle Scholar
Catcheside, D. G., (1974). Fungal genetics. Annual Review of Genetics 8, 279–300.CrossRefGoogle ScholarPubMed
de Serres, F. J. (1971). Mutability of UV-sensitive strains of Neurospora crassa. Genetics 68, s14–s15.Google Scholar
Howard-Flanders, P. & Boyce, R. P. (1966). DNA repair and genetic recombination: studies on mutants of E. coli defective in these processes. Radiation Research (supplement 6), 156–184.Google ScholarPubMed
Nemerofsky, A. (1975). The interactive effect of UV irradiation and 5-BU at the rib-1 locus in N. crassa. Canadian Journal of Genetics and Cytology 17, 275–278.CrossRefGoogle Scholar
Schroeder, A. L. (1974). Properties of a UV-sensitive Neurospora strain defective in pyrimidine dimer excision. Mutation Research 24, 9–16.CrossRefGoogle ScholarPubMed
Setlow, R. & Carrier, W. (1964). The disappearance of thymine dimers from DNA, an error correcting mechanism. Proceedings of the National Academy of Sciences, U.S.A. 51, 226–231.CrossRefGoogle ScholarPubMed
Stadler, D. & Smith, D. D. (1968). A new mutation in Neurospora for sensitivity to U.V. light. Canadian Journal of Genetics and Cytology 10, 916–919.CrossRefGoogle Scholar
Tatum, E. L., Barratt, R. W., Fries, N. & Bonner, D. (1950). Biochemical strains of Neurospora produced by physical and chemical treatment. American Journal of Botany 37, 38–46.Google Scholar
Westergaard, M. & Mitchell, H. K. (1947). Neurospora. V. A synthetic medium favouring sexual reproduction. American Journal of Botany 34, 573–577.CrossRefGoogle Scholar
Witkin, E. M. (1966). Radiation induced mutation and their repair. Science 154, 1345–1347.CrossRefGoogle Scholar
Witkin, E. M. (1969 a). Ultraviolet induced mutation and DNA repair. Annual Review of Genetics 3, 525–552.CrossRefGoogle Scholar
Witkin, E. M. (1969 b). The role of DNA repair in recombination and mutagenesis. Proceeding X11th International Congress of Genetics 3, 225–245.Google Scholar
Worthy, T. E. & Epler, J. L. (1972). Repair of ultraviolet light-induced damage to the DNA of N. crassa. Journal of Bacteriology 110, 1010–1016.CrossRefGoogle Scholar
Worthy, T. E. & Epler, J. L. (1973 a). Characterization of excision repair in N. crassa. Journal of Bacteriology 115, 498–505.CrossRefGoogle Scholar
Worthy, T. E. & Epler, J. L. (1973 b). Biochemical basis of radiation-sensitivity in mutants of Neurospora crassa. Mutation Research 19, 167–173.CrossRefGoogle ScholarPubMed