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The effect of unbalanced growth on recombinant formation in E. coli

Published online by Cambridge University Press:  14 April 2009

J. D. Gross
J. D. Gross
Affiliation:
Medical Research Council Microbial Genetics Research Unit, Hammersmith Hospital, London, W. 12
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When zygotes formed by conjugation between Hfr and F- cells of E. coli are transferred from an amino-acid rich medium to one lacking amino-acids the probability of genetic recombination is reduced. This result is interpreted as indicating that recombination can only occur during a limited period after zygote formation and that the metabolic imbalance caused by the change of medium leads to temporary inhibition of recombination. By an hour after zygote formation the yield of recombinants is no longer affected by transfer to amino-acid free medium.

Type
Research Article
Information
Genetics Research , Volume 4 , Issue 3 , November 1963 , pp. 457 - 462
Copyright
Copyright © Cambridge University Press 1963

References

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