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Functional analysis of host-specificity mutants in Escherichia coli

Published online by Cambridge University Press:  14 April 2009

S. W. Glover
S. W. Glover
Affiliation:
M.R.C. Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR

Summary

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Evidence from a functional analysis of host-specificity mutants in merodiploids is presented which supports the suggestion that three genes, hss, hsr and hsm, are necessary for the expression of host-controlled restriction and modification. The host-specificity phenotype expressed by the merodiploids provides evidence that at least two genes, hss and hsr, are concerned in the expression of host-specific restriction of DNA and one of these genes, hss, is responsible for the strain specificity of the restriction enzyme. A class of modification-deficient mutants isolated from restriction-deficient, modification-proficient mutants, was also tested for complementation in merodiploids and the phenotype of these merodiploids provides evidence that at least two genes, hss and hsm, are concerned in the expression of host-specific modification of DNA and one of these genes, hss, is responsible for the strain specificity of the modification enzyme. How these three genes function at the molecular level is discussed in terms of models based on the interaction of subunits to form oligomeric enzymes.

Type
Research Article
Information
Genetics Research , Volume 15 , Issue 2 , April 1970 , pp. 237 - 250
Copyright
Copyright © Cambridge University Press 1970

References

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