Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-08T01:22:24.544Z Has data issue: false hasContentIssue false
  • English
  • Français

Production of auxotrophic mutants in ferns

Published online by Cambridge University Press:  14 April 2009

Peter S. Carlson
Peter S. Carlson
Affiliation:
Department of Biology, Yale University, New Haven, Connecticut 06520
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A method has been devised for the selection of auxotrophic mutants in higher plants. The method depends upon the incorporation of BUdR into the DNA of non-auxotrophic cells and upon its lack of incorporation into the DNA of auxotrophic cells. A wide range of auxotrophic types were recovered.

Type
Short Papers
Information
Genetics Research , Volume 14 , Issue 3 , December 1969 , pp. 337 - 339
Copyright
Copyright © Cambridge University Press 1969

References

REFERENCES

Guha, S. & Maheshwari, S. C. (1966). Cell division and differentiation of embryos in the pollen grains of Datura, in vitro. Nature, Lond. 212, 97–98.Google Scholar
Holliday, R. (1956). A new method for the identification of biochemical mutants of microorganisms. Nature, Lond. 178, 987.CrossRefGoogle Scholar
Nakata, K. & Tanaka, M. (1968). Differentiation of embryoids from developing germ cells in anther culture of tobacco. Jap. J. Genet. 43, 65–71.Google Scholar
Nitsch, J. P. & Nitsch, C. (1969). Haploid plants from pollen grains. Science, N. Y. 163, 85–87.Google Scholar
Puck, T. T. & Kao, F. T. (1967). Genetics of somatic mammalian cells. V. Treatment with 5-bromodeoxyuridine and visible light for isolation of nutritionally deficient mutants. Proc. natn. Acad. Sci. U.S.A. 58, 1227–1234.Google Scholar
Stahl, F. W., Crosemann, J. M., Okun, L., Fox, E. & Laird, C. (1961). Radiation-sensitivity of bacteriophage containing 5-bromodeoxyuridine. Virology 13, 98–104.Google Scholar
Vasil, V. & Hildebrandt, A. C. (1965). Differentiation of tobacco plants from single, isolated cells in microculture. Science, N. Y. 150, 889–892.Google Scholar