Hostname: page-component-7479d7b7d-fwgfc Total loading time: 0 Render date: 2024-07-10T16:25:33.468Z Has data issue: false hasContentIssue false
  • English
  • Français

The relationship between the transfer systems of some bacterial plasmids

Published online by Cambridge University Press:  14 April 2009

Guillermo Alfaro  and
Neil Willetts
Guillermo Alfaro
Affiliation:
M.R.C. Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh, Scotland
Neil Willetts
Affiliation:
M.R.C. Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh, Scotland

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The relationship between the transfer systems of several plasmids was investigated, using as criteria complementation of a series of transfer-deficient Flac mutants, the efficiency of plating of F-specific phages, and identification of surface exclusion systems. The transfer systems of ColV2 and ColVBtrp were similar to that of F except for surface exclusion: ColVBtrp specified a surface exclusion system different from that of F, and ColV2 did not specify any detectable system. The transfer system of R1–19 was also similar to that of Flac, but the products of traA, traI and traJ were plasmid-specific. R1–19 determined the same surface exclusion system as ColVBtrp, and this was under the control of the transfer inhibitor. The transfer systems of ColIbdrd and Flac were unrelated. ColEl was transferred by Flac traI− mutants, but not by mutants in other cistrons.

Type
Research Article
Information
Genetics Research , Volume 20 , Issue 3 , December 1972 , pp. 279 - 289
Copyright
Copyright © Cambridge University Press 1972

References

REFERENCES

Achtman, M., Willetts, N. S. & Clark, A. J. (1971). Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. Journal of Bacteriology 106, 529–538.CrossRefGoogle Scholar
Achtman, M., Willetts, N. S. & Clark, A. J. (1972). Conjugational complementation analysis of transfer-deficient mutants of Flac in E. coli. Journal of Bacteriology 110, 831–842.CrossRefGoogle Scholar
Clowes, R. C. (1963). Colicin factors and episomes. Genetical Research 4, 162–165.CrossRefGoogle Scholar
Clowes, R. C. (1972). Molecular nature of bacterial plasmids. Bacterial Plasmids and Antibiotic Resistance (ed. by Krčméry, V., Rosival, L. and Watanabe, T.), pp. 283–296. Published by Avicenum (Czechoslovak Medical Press, Prague) and Springer-Verlag (Berlin).CrossRefGoogle Scholar
Egawa, R. & Hirota, Y. (1962). Inhibition of fertility by multiple drug resistance factor in E. coli K 12. Japanese Journal of Genetics 37, 66–69.Google Scholar
Finnegan, D. J. & Willetts, N. S. (1971). Two classes of F lac mutants insensitive to transfer inhibition by an F-like R factor. Molecular and General Genetics 111, 256–264.CrossRefGoogle Scholar
Fredericq, P. (1969). The recombination of colicinogenic factors with other episomes and plasmids. In Bacterial Episomes and Plasmids, CIBA Foundation Symposium (ed. Wolstenholme, G. and O'Connor, M.), pp. 163–174.Google Scholar
Goebel, W. (1972). Replication of the DNA of ColEl at the restrictive temperature in a DNA replication mutant thermosensitive for DNA polymerase III. Nature New Biology 237, 67–70.CrossRefGoogle Scholar
Gross, J. D. (1963). The effect of unbalanced growth on recombinant formation in Escherichia coli. Genetical Research 4, 457–462.CrossRefGoogle Scholar
Hashimoto, H. & Hirota, Y. (1966). Gene recombination and segregation of resistance factor R in E. coli. Journal of Bacteriology 91, 57–62.CrossRefGoogle Scholar
Helinski, D. R. & Clewell, D. B. (1971). Circular DNA. Annual Review of Biochemistry 25, 899–942.CrossRefGoogle Scholar
Kahn, P. & Helinski, D. R. (1964). Relationship between colicinogenic factors El, V, and an F factor in E. coli. Journal of Bacteriology 88, 1573–1579.CrossRefGoogle Scholar
Kingsbury, D. T. & Helinski, D. R. (1970). DNA polymerase as a requirement for the maintenance of the bacterial plasmid ColE1. Biochemical and Biophysical Research Communications 41, 1538–1544.CrossRefGoogle Scholar
Lawn, A. M. & Meynell, E. (1970). Serotypes of sex pili. Journal of Hygiene 68, 683–694.Google ScholarPubMed
Lawn, A. M., Meynell, E. & Cooke, M. (1971). Mixed infections with bacterial sex factors: sex pili of pure and mixed phenotype. Annals Institut Pasteur 120, 3–8.Google ScholarPubMed
Lederberg, J., Cavalli, L. L. & Lederberg, E. M. (1952). Sex compatibility in E. coli. Genetics 37, 720–730.CrossRefGoogle Scholar
Low, B. & Wood, T. H. (1965). A quick and efficient method for interruption of bacterial conjugation. Genetical Research 6, 300–303.CrossRefGoogle ScholarPubMed
MacFarren, A. C. & Clowes, R. C. (1967). A comparative study of two F-like colicin factors, ColV2 and ColV3, in E. coli K12. Journal of Bacteriology 94, 365–377.CrossRefGoogle Scholar
Meynell, E. & Datta, N. (1967). Mutant drug resistance factors of high transmissibility. Nature 214, 885–887.CrossRefGoogle ScholarPubMed
Meynell, E., Meynell, G. G. & Datta, N. (1968). Phylogenetic relationships of drug resistance factors and other transmissible bacterial plasmids. Bacteriological Reviews 32, 55–83.CrossRefGoogle ScholarPubMed
Monk, M. & Clowes, R. C. (1964). Transfer of the colicin I factor in E. coli K12 and its interaction with the F fertility factor. Journal of General Microbiology 36, 365–384.CrossRefGoogle Scholar
Nagel de Zwaig, R. & Puig, J. (1964). The genetic behaviour of colicinogenic factor E1. Journal of General Microbiology 36, 311–321.CrossRefGoogle Scholar
Negrotti, T. & Nagel De Zwaig, R. (1972). Interactions between colicinogenic factor B and F-type R factors: isolation of a mutant Flac factor insensitive to the effect of colicinogenic factor B. Genetics 70, 205–213.CrossRefGoogle Scholar
Nishimura, Y., Ishibashi, M., Meynell, E. & Hirota, Y. (1967). Specific piliation directed by a fertility factor and a resistance factor of E. coli. Journal of General Microbiology 49, 89–98.CrossRefGoogle Scholar
Taylor, A. L. (1970). Current linkage map of E. coli. Bacteriological Reviews 34, 155–175.CrossRefGoogle Scholar
Willetts, N. S. (1970). The interaction of an I-like R factor and transfer-deficient mutants of Flac in Escherichia coli K12. Molecular and General Genetics 108, 365–373.CrossRefGoogle Scholar
Willetts, N. S. (1971). Plasmid specificity of two proteins required for conjugation in Escherichia coli K12. Nature New Biology 230, 183–185.CrossRefGoogle Scholar
Willetts, N. S. & Achtman, M. (1972). A genetic analysis of transfer by the E. coli sex factor F, using PI transductional complementation. Journal of Bacteriology 110, 843–851.CrossRefGoogle Scholar
Willetts, N. S. & Finnegan, D. J. (1970). Characteristics of E. coli K12 strains carrying both an F prime and an R factor. Genetical Research 16, 113–122.CrossRefGoogle Scholar
Willetts, N. S. & Finnegan, D. J. (1972). A genetic analysis of conjugational transfer and its control. Bacterial Plasmids and Antibiotic Resistance (ed. by Krčméry, V., Rosival, L. and Watanabe, T.), pp. 173–177. Published by Avicenum (Czechoslovak Medical Press, Prague) and Springer-Verlag (Berlin).CrossRefGoogle Scholar