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Molecular Nosocomial Epidemiology: High Speed Typing of Microbial Pathogens by Arbitrary Primed Polymerase Chain Reaction Assays

Published online by Cambridge University Press:  02 January 2015

Alex van Belkum ,
Willem van Leeuwen ,
Jan Kluytmans  and
Henri Verbrugh
Alex van Belkum*
Affiliation:
Department of Bacteriology, University Hospital, Rotterdam, The Netherlands
Willem van Leeuwen
Affiliation:
Department of Bacteriology, University Hospital, Rotterdam, The Netherlands
Jan Kluytmans
Affiliation:
Department of Bacteriology, University Hospital, Rotterdam, The Netherlands
Henri Verbrugh
Affiliation:
Department of Bacteriology, University Hospital, Rotterdam, The Netherlands
*
Dr. Molewaterplein 40, Department of Bacteriology, University Hospital Rotterdam, 3015 GD Rotterdam, The Netherlands
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Abstract

Arbitrary primed polymerase chain reaction (AP PCR) assays are suited for the discrimination of isolates of all clinically relevant bacterial species. In a hospital setting, this type of DNA amplification test can be used for the timely detection of ongoing nosocomial outbreaks. For rapid screening of isolates of many medically important bacterial species, including Acinetobacter baumannii, Staphylococcus aureus, Serratia marcescens, Xanthomonas maltophilia and others, a single AP PCR assay can be used as a primary typing screen for genetic relatedness. In combination with epidemiological data, AP PCR testing is particularly useful for identifying true outbreaks caused by a single strain.

Type
Molecular Hospital Epidemiology
Information
Infection Control & Hospital Epidemiology , Volume 16 , Issue 11 , November 1995 , pp. 658 - 666
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1995

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