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Usefulness of Adenosinetriphosphate Bioluminescence Assay (ATPmetry) for Monitoring the Reprocessing of Endoscopes

Published online by Cambridge University Press:  20 October 2015

Pierre Batailler ,
Philippe Saviuc ,
Romain Picot-Gueraud ,
Jean-Luc Bosson  and
Marie-Reine Mallaret
Pierre Batailler*
Affiliation:
Grenoble University Hospital Center, Public Health Center, Hygiene Unit and Hospital Risk Management, Grenoble, France
Philippe Saviuc
Affiliation:
Grenoble University Hospital Center, Public Health Center, Hygiene Unit and Hospital Risk Management, Grenoble, France
Romain Picot-Gueraud
Affiliation:
Grenoble University Hospital Center, Public Health Center, Hygiene Unit and Hospital Risk Management, Grenoble, France
Jean-Luc Bosson
Affiliation:
French Institute of Health and Medical Research CIC, Grenoble, France Univ. Grenoble Alpes, Laboratory for Techniques for Biomedical Engineering and Complexity Management—Informatics, Mathematics, and Applications, Grenoble, France
Marie-Reine Mallaret
Affiliation:
Grenoble University Hospital Center, Public Health Center, Hygiene Unit and Hospital Risk Management, Grenoble, France Univ. Grenoble Alpes, Laboratory for Techniques for Biomedical Engineering and Complexity Management—Informatics, Mathematics, and Applications, Grenoble, France
*
Address correspondence to Pierre Batailler, MD, Unité d’Hygiène Hospitalière, CHU de Grenoble, CS 10217-38043 Grenoble CEDEX 9 (pbatailler@chu-grenoble.fr).
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Abstract

OBJECTIVE

To assess the diagnostic value of an adenosinetriphosphate bioluminescence assay (ATPmetry) to monitor the effectiveness of the reprocessing of endoscopes compared with microbiologic sampling.

DESIGN

Diagnostic study.

SETTING

A 2,200-bed teaching hospital performing 5,000 to 6,000 endoscopic procedures annually.

INCLUSION CRITERIA

All samples from bronchial or gastrointestinal endoscopes whatever the context.

METHODS

Samples for microbiologic analysis and ATPmetry measurements were taken when each endoscope was inspected following reprocessing. Sampling was performed by flushing each endoscope with 300 mL Neutralizing Pharmacopeia Diluent thiosulfate rinsing solution divided equally between the endoscope channels. For each endoscope a series of 3 ATPmetry measurements were made on a vial containing the first jet from each channel and a second series on the whole sample.

RESULTS

Of 165 samples from endoscopes, 11 exceeded the acceptability threshold of 25 colony-forming units/endoscope. In the first jet collected, the median (interquartile range) level of ATPmetry was 30.5 (15.3–37.7) relative light units (RLU) for samples with 25 or fewer colony-forming units compared with 37.0 (34.7–39.3) RLU for samples with more than 25 colony-forming units (P=.008). For the whole sample, the median (interquartile range) level of ATPmetry was 24.8 (14.3–36.3) RLU and 36.3 (36.0–38.3) RLU (P=.006), respectively. After adjusting on the batch of cleansing solution used, no difference in ATPmetry values was found between microbiologically acceptable and unacceptable samples.

CONCLUSION

ATPmetry cannot be used as an alternative or complementary approach to microbiologic tests for monitoring the reprocessing of endoscopes in France

Infect. Control Hosp. Epidemiol. 2015;36(12):1437–1443

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 36 , Issue 12 , December 2015 , pp. 1437 - 1443
Copyright
© 2015 by The Society for Healthcare Epidemiology of America. All rights reserved 

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