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Chitooligosaccharides enhance cold tolerance by repairing photodamaged PS II in rice

Published online by Cambridge University Press:  26 October 2018

Jiachun Zhou ,
Qiao Chen ,
Yang Zhang ,
Liqiang Fan Open the ORCID record for Liqiang Fan [Opens in a new window] ,
Zhen Qin ,
Qiming Chen ,
Yongjun Qiu ,
Lihua Jiang  and
Liming Zhao
Jiachun Zhou
Affiliation:
State Key Laboratory of Bioreactor Engineering, RandD Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai 200237, China
Qiao Chen
Affiliation:
State Key Laboratory of Bioreactor Engineering, RandD Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai 200237, China Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCIBT), Shanghai 200237, China
Yang Zhang
Affiliation:
State Key Laboratory of Bioreactor Engineering, RandD Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai 200237, China Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCIBT), Shanghai 200237, China
Liqiang Fan*
Affiliation:
State Key Laboratory of Bioreactor Engineering, RandD Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai 200237, China Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCIBT), Shanghai 200237, China
Zhen Qin
Affiliation:
State Key Laboratory of Bioreactor Engineering, RandD Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai 200237, China
Qiming Chen
Affiliation:
State Key Laboratory of Bioreactor Engineering, RandD Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai 200237, China
Yongjun Qiu
Affiliation:
State Key Laboratory of Bioreactor Engineering, RandD Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai 200237, China Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCIBT), Shanghai 200237, China
Lihua Jiang
Affiliation:
State Key Laboratory of Bioreactor Engineering, RandD Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai 200237, China
Liming Zhao*
Affiliation:
State Key Laboratory of Bioreactor Engineering, RandD Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai 200237, China Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCIBT), Shanghai 200237, China
*
Author for correspondence: Liming Zhao and Liqiang Fan, E-mail: zhaoliming@ecust.edu.cn, fanglq@ecust.edu.cn
Author for correspondence: Liming Zhao and Liqiang Fan, E-mail: zhaoliming@ecust.edu.cn, fanglq@ecust.edu.cn
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Abstract

Chitooligosaccharides (COS) are multi-functional foods and nutrients and environmentally friendly biological abiotic-resistance inducing agents for plants. In the current study, the effects and possible mechanisms of COS on improving the cold resistance of rice (II YOU 1259) seedlings were investigated. Compared with the control, a COS pre-soaking treatment enhanced photosynthesis, reduced oxidation damage and led to accumulation of more osmotic regulation substances under chilling treatment. In addition, a novel Deg/HtrA family serine endopeptidase (DegQ) gene, related to COS enhanced rice cold resistance, was identified. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed that transcription of DegQ and psbA (D1 protein encoding gene) were up-regulated in a time-dependent manner by COS treatment under cold stress. With increasing expression of the D1 protein, chlorophyll b content was enhanced correspondingly. The current results suggest that COS could enhance cold stress tolerance of rice by repairing the photodamaged photosystem II, altering osmotic regulation and reducing oxidation damage.

Keywords

Chitooligosaccharidescold tolerancePS II repairingricestress-responsive gene
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 156 , Issue 7 , September 2018 , pp. 888 - 899
Copyright
Copyright © Cambridge University Press 2018 

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