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Chlorophyll fluorescence as a rapid test for reaction to urea herbicides in winter wheat

Published online by Cambridge University Press:  27 March 2009

Margaret Harris
Affiliation:
Department of Agricultural Botany, The Queen's University of Belfast, Northern Ireland
M. S. Camlin
Affiliation:
Department of Agricultural Botany, The Queen's University of Belfast, Northern Ireland

Summary

A simple and rapid test is described which can be used to determine tolerance to metoxuron and chlortoluron herbicides in winter wheat (Triticum aestivum L.) cultivars. Seedlings 5 days old were treated by root application of herbicide for 24 h and tolerant and susceptible cultivars were identified by comparing the chlorophyll fluorescence induction curves for both herbicide-treated and control plants after 7 days. The method was thus capable of detecting cultivar tolerance or susceptibility to the herbicides within 12 days of sowing the seed, providing a rapid and effective screening method. Possible uses for this new test are discussed.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1988

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References

Ahrens, W. H., Arntzen, C. J. & Stoller, E. W. (1981). Chlorophyll fluorescence assay for the determination of triazine resistance. Weed Science 29, 316322.Google Scholar
Cadahia, E., Ducruet, J.M. & Gaillardon, P. (1982). Whole leaf fluorescence as a quantitative probe of the herbicide chlortoluron in wheat. Chemosphere 11, 445450.Google Scholar
Ducruet, J.M. & Gasquez, J. (1978). Observation de la fluorescence sur feuille entière et mise en évidence de la résistance chloroplastique à l'atrazine chez Chenopodium album L. et Poa annua L. Chenwsphere 8, 691696.Google Scholar
Flint, C. (1986). Winter cereals – autumn herbicides. Farmers Weekly Supplement, 29 08.Google Scholar
Habash, D., Percival, M. P. & Baker, N. R. (1985). Rapid chlorophyll fluorescence technique for the study of penetration of photosynthetically active herbicides into leaf tissue. Weed Research 25, 389395.Google Scholar
Izawa, S. & Good, N. E. (1965). The number of sites sensitive to 3-(3,4-diehlorophenyl)-l, l-dimethyl urea, 3-(4-chlorophenyl)-l, 1-dimethyl urea and 2-chloro-4(2- propylamino)-6-ethylamino-5-triazine in isolated chloroplasts. Biochimica et Biophysica Ada 102, 2038.Google Scholar
Richard, E. P. Jun, Goss, J. R., Arntzen, C. J. L. & Slife, F. W. (1983). Determination of herbicide inhibition of photosynthetic electron transport by fluorescence. Weed Science 31, 361367.Google Scholar
Sandmann, G. & Boger, P. (1986). Sites of herbicide inhibition at the photosynthetic apparatus. In Photosynthesis III: Photosynthetic Membranes and Light Harvesting Systems (Ed. Staehelin, L. A. and Arntzen, C. J.), Encyclopaedia of Plant Physiology, New Series Volume 19, pp. 599602. Berlin, Heidelberg, New York, Tokyo: Springer-Verlag.Google Scholar
Shaw, D. R., Peeper, T. F. & Nofziger, D. L. (1985). Comparison of chlorophyll fluorescence and fresh weight as herbicide bioassay techniques. Weed Science 33, 2933.Google Scholar
Shaw, D. R., Peeper, T. F. & Nofziger, D. L. (1986). Evaluation of chlorophyll fluorescence parameters for an intact-plant herbicide bioassay. Crop Science 26, 756760.Google Scholar
Shimabukuro, R. H. & Swanson, H. R. (1969). Atrazine metabolism, selectivity, and mode of action. Journal of Agricultural and Food Chemistry 17, 199205.Google Scholar