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Activation of milk lipase by serum proteins: possible role in the occurrence of lipolysis in raw bovine milk

Published online by Cambridge University Press:  01 June 2009

Roger A. Clegg
Roger A. Clegg
Affiliation:
The Hannah Research Institute, Ayr, Scotland, K A6 5HL
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Summary

The increase in unesterified fatty acid content of unpasteurized bulk milk in storage at 4 °C in the presence of known effectors of bovine milk lipoprotein lipase originating from bovine serum was studied. Bovine serum and high density lipoprotein (HDL) caused an increase in developed unesterified fatty acid levels whilst lipoprotein-free serum, apo-HDL, all individual apo-HDL tested, and the unfractionated C-peptide fraction were without lipolytic effect. In the presence of HDL-lipids, 2 C-peptides stimulated considerable lipolysis, as did the combination of HDL-lipid with unfractionated C-peptides. These characteristics of unesterified fatty acid development could be duplicated in milk whose endogenous lipolytic activity had been destroyed by heat treatment (75 °C for 5 min) and then restored by addition of purified bovine milk lipoprotein lipase. Radioactively labelled glycerol trioleate in milk was not hydrolysed in the same way as milk fat on the addition of serum liproproteins.

Type
Original Articles
Information
Journal of Dairy Research , Volume 47 , Issue 1 , February 1980 , pp. 61 - 70
Copyright
Copyright © Proprietors of Journal of Dairy Research 1980

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