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Antibiotic survey of Lactococcus lactis strains to six antibiotics by Etest, and establishment of new susceptibility-resistance cut-off values

Published online by Cambridge University Press:  30 April 2007

Ana Belén Flórez ,
Morten Danielsen ,
Jenni Korhonen ,
Joanna Zycka ,
Atte von Wright ,
Jacek Bardowski  and
Baltasar Mayo
Ana Belén Flórez
Affiliation:
Instituto de Productos Lácteos de Asturias (CSIC), Carretera de Infiesto s/n, 33300-Villaviciosa, Asturias, Spain
Morten Danielsen
Affiliation:
Chr. Hansen A/S, Bøge Allé 10-12, DK-2970 Hørsholm, Denmark
Jenni Korhonen
Affiliation:
Institute of Applied Biotechnology, University of Kuopio, Bioteknia 2, PO Box 1627, Kuopio, Finland
Joanna Zycka
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland
Atte von Wright
Affiliation:
Institute of Applied Biotechnology, University of Kuopio, Bioteknia 2, PO Box 1627, Kuopio, Finland
Jacek Bardowski
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland
Baltasar Mayo*
Affiliation:
Instituto de Productos Lácteos de Asturias (CSIC), Carretera de Infiesto s/n, 33300-Villaviciosa, Asturias, Spain
*
*For correspondence; e-mail: baltasar.mayo@ipla.csic.es
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Abstract

In order to establish cut-off values for Lactococcus lactis to six antibiotics to distinguish susceptible and intrinsically resistant strains from those having acquired resistances, the minimum inhibitory concentration (MIC) of tetracycline, erythromycin, clindamycin, streptomycin, chloramphenicol and vancomycin was determined in 93 different Lc. lactis strains using the Etest. These bacterial strains were originally isolated from dairy and animal sources in widely separated geographical locations. Cut-offs were defined on the basis of the distribution of the MICs frequency of the studied antibiotics, which in the absence of acquired determinants should approach to a normal statistical distribution. In general, the new cut-off values proposed in this study are higher than previously defined (European Commission, 2005. The EFSA Journal 223, 1–12). Based on these new values, all the strains tested were susceptible to erythromycin, chloramphenicol and vancomycin, and 79 susceptible to all six antibiotics. However, 11 strains (around 12%) were considered resistant to tetracycline (six of which had been identified after screening of a large collection of lactococci strains for tetracycline resistance) and five (5·4%) resistant to streptomycin. Of these, two fish isolates proved to be resistance to both tetracycline and streptomycin. From the tetracycline resistant strains, tet(M) and mosaic tet(L/S) genes were amplified by PCR, demonstrating they harboured acquired antibiotic resistance determinants.

Keywords

Lactococcus lactissusceptibility testingminimum inhibitory concentrationEtestantimicrobial resistancetetracycline resistance
Type
Research Article
Information
Journal of Dairy Research , Volume 74 , Issue 3 , August 2007 , pp. 262 - 268
Copyright
Copyright © Proprietors of Journal of Dairy Research 2007

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