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Antioxidant and cholesterol assimilation activities of selected lactobacilli and lactococci cultures

Published online by Cambridge University Press:  29 July 2009

Shalini Jain ,
Hariom Yadav  and
Pushpalata Ravindra Sinha
Shalini Jain
Affiliation:
Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, India
Hariom Yadav*
Affiliation:
Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, India
Pushpalata Ravindra Sinha
Affiliation:
Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, India
*
*For correspondence; e-mail: yadavhariom@gmail.com
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Abstract

In present study, three strains of lactic acid bacteria (LAB) viz. Lactobacillus casei, Lactobacillus acidophilus and Lactococcus lactis and milk fermented with these strains have been studied for antioxidant and cholesterol assimilation activities in-vitro and in-vivo, in addition to the effect on total lactobacilli, lactococci and coliform counts into the gut of mice fed with diets supplemented by fermented milk. All three selected strains exhibited potent 2,2-diphenyl-1-picrylhydrazyl, malonaldialdehyde and hydrogen peroxide radical scavenging abilities as well as inhibition of linoleic acid peroxidation activity. These activities were highest in Lb. casei as followed by Lb. acidophilus and Lc. lactis. In addition, these bacterial cultures also exhibited good in-vitro cholesterol assimilation potential. Oral administration to mice of milk fermented with selected LAB strains, slightly decreased blood cholesterol, increased colonization of total lactobacilli and lactococci, and decreased coliforms in the intestinal tissues as well as faecal samples. These results indicate that, selected LAB strains have good antioxidant, hypocholesterolemic and coliform removal activities. It may suggest that, a novel functional food can be obtained by supplementation of selected LAB in milk, which may have various health beneficial properties such as antioxidant and hypocholesterolemic activities.

Keywords

Lactic acid bacteriaLactobacillusAntioxidant activityCholesterol assimilation
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 4 , November 2009 , pp. 385 - 391
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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